ABSTRACT
Moringa oleifera Lam, commonly known as moringa, is a plant widely used both as a human food and for medicinal purposes around the world. This research aimed to evaluate the efficacy of the aqueous extract of Moringa oleifera leaves (MoAE) and benzyl isothiocyanate (BIT) in rats with induced breast cancer. Cancer was induced with 7,12-dimethylbenz[a]anthracene (DMBA) at a dose of 60 mg/kg by orogastric gavage once only. Forty-eight rats were randomly assigned to eight groups, each consisting of six individuals. The control group (healthy) was called Group I. Group II received DMBA plus saline. In addition to DMBA, Groups III, IV, and V received MoAE at 100, 250, and 500 mg/kg/day, respectively, while Groups VI, VII, and VIII received BIT at 5, 10, and 20 mg/kg/day, respectively. Treatment was carried out for 13 weeks. Secondary metabolite analysis results identified predominantly quercetin, caffeoylquinic acid, neochlorogenic acid, vitexin, and kaempferol, as well as tropone, betaine, loliolide, and vitexin. The administration of MoAE at a dose of 500 mg/kg and BIT at 20 mg/kg exhibited a notable decrease in both the total tumor count and the cumulative tumor weight, along with a delay in their onset. Furthermore, they improved the histological grade. A significant decrease in serum levels of VEGF and IL-1ß levels was observed (p < 0.001) with a better effect demonstrated with MoAE at 500 mg/kg and BIT at 20 mg/kg. In conclusion, this study suggests that both the aqueous extract of Moringa oleifera leaves and the benzyl isothiocyanate possess antitumor properties against mammary carcinogenesis, and this effect could be due, at least in part, to the flavonoids and isothiocyanates present in the extract.
Subject(s)
Moringa oleifera , Mice , Rats , Humans , Animals , Moringa oleifera/chemistry , Plant Extracts/chemistry , Chromatography, High Pressure Liquid , Isothiocyanates/chemistry , Phytochemicals/pharmacology , Phytochemicals/analysis , Carcinogenesis , Plant Leaves/chemistryABSTRACT
La investigación tuvo como objetivo evaluar la eficacia y seguridad de supositorios de aceite de Copaifera officinalis L. "copaiba" en pacientes con diágnóstico definitivo de crisis hemorroidal aguda. El ensayo fue clínico prospectivo, controlado, comparativo, aleatorio y se realizó en el Instituto de Investigaciones Clínicas de la Facultad de Medicina de UNMSM. y Servicio de Gastroenterología del Hospital Nacional Dos de Mayo. Los pacientes evaluados presentaban diagnóstico definitivo de crisis hemorroidal aguda. El diagnóstico se realizó mediante estudio clínico y proctoscópico antes y después del tratamiento, además de exámenes auxiliares serológicos; 60 pacientes fueron enrolados voluntariamente al protocolo cumpliendo con los criterios éticos de investigación: los cuales fueron distribuidos en 3 grupos de 20 casos cada uno, administrándoseles supositorios de copaiba 120 mg bid, copaiba 120 mg/lidocaína 30 mg bid, y Prednisolona caproato 1,3 mg/Cincocaína clorhídrato 1 mg respectivamente durante 7 días. Los datos obtenidos como el alivio de la sintomatología, disminución del tamaño de las hemorroides y presencia de eventos adversos fueron evaluados mediante técnicas multivariadas (p<0,05). Se logró mejoría clínica y desaparición de síntomas de la enfermedad, sin efectos adversos significativos; dos presentaron dolor y ardor anal de manera transitoria, y no requirió suspensión de la medicación. Se concluyó que los supositorios de aceita de Copaifera officinalis es una alternativa eficaz y segura en el tratamiento de la crisis hemorroidal aguda.
The present study aimed to evaluate the efficacy and safety of oil suppositories Copaifera officinalis L. "copaiba" in patients with a final diagnosis of acute hemorrhoidal crisis. The trial was prospective, controlled, comparative, randomized and performed in the Clinical Research Institute of the Faculty of Medicine UNMSM Gastroenterology and National Hospital Dos de Mayo. Patients evaluated had definite diagnosis of acute hemorrhoidal crisis. The diagnosis was made by clinical and proctoscópico before and after treatment, plus auxiliary serologic tests, 60 patients were enrolled voluntarily complying with the protocol ethical research, which were divided into 3 groups of 20 cases each, was administered suppositories 120 mg bid copaiba 120 mg/lidocaine 30mg bid, and prednisole caproate 13 mg/Cinchocaine hydrochloride 1 mg respectively for 7 days. Data obtained as relief of symptoms, decrease the size of hemorrhoids and presence of adverse events were assessed by multivariate techniques (p<0.05). was achieved clinical improvement and disappearance of symptoms of the symptoms of the disease, without significant adverse effects, two had anal burning pain temporaly, and did not require drug discontinuation. It was concluded that oil suppositories Copaifera officinalis is an effective and safe alternative in the treatment of acute hemorrhoidal crisis.
Subject(s)
Fabaceae , Hemorrhoids , Suppositories , Plant OilsABSTRACT
Objetivo: Evaluar el efecto tóxico del extracto acuoso de la raíz del Lonchocarpus nicou (Aubl) D.C. "Barbasco" sometido a 100 grados C de temperatura durante 5, 10, 30 y 60 minutos de ebullición, al ser administrado oralmente durante 30 días a ratas normales. Materiales y Métodos: Estudio experimental, con grupos equivalentes randomizados, ciego para quien administró la solución, el analista de laboratorio, el anatomopatólogo y el estadístico. Se sometieron a 100 grados C de temperatura trozos pequeños de raíz desecados con agua destilada, obteniéndose extractos a 5, 10, 30 y 60 minutos de ebullición para efectuar ensayos fitoquímicos comparativos en cromatografía de capa fina, y evaluar del efecto tóxico en cuarenta ratas Holtzmann. El peso de las ratas fue (190±10 gr), se las dividió aleatoriamente en cinco grupos: al primero se le administró suero fisiológico 2 mL/kg, y a los otros grupos 10 mg/kg de extracto respectivamente durante 30 días. A continuación se extrajo muestra de sangre para observación hematológica, bioquímica, y luego se sacrificó los animales, retirándose cerebro, hígado, y riñón, los que se conservaron en formol al 10 por ciento para estudio histopatológico. Los datos obtenidos se analizaron mediante pruebas descriptivas y analíticas, considerando significativos una p<0,05. Resultados: Los metabolitos secundarios disminuyeron con la ebullición, los alcaloides del barbasco desaparecieron y los flavonoides tipo isoflavonas disminuyeron notoriamente con la calificación cualitativa. A mayor tiempo de exposición a la temperatura, se presentó menor daño en el perfil hepático. Conclusiones: En condiciones experimentales se ha observado en ratas normales disminución del efecto tóxico inducido por el extracto acuoso de barbasco sometido a altas temperatura, acción dependiente del tiempo de ebullición.
Objective: Assessing the toxic effect of aqueous extract of Lonchocarpus nicou roots (Aubl) S.D. "Barbasco" after being exposed at 100 grades C temperature during: 5, 10, 30, 60 minutes of boiling condition and then being administered during 30 days by oral intake in normal rats. Material and methods: The present research is experimental and prospective, with similar groups distributed randomly. It is blind not only for the ones who administered the concentration, made the lab analysis and the anatomopathologic examination but also for the ones who made the data processing. The extracts was exposed at 100 grades C of temperature then it was gotten extracts with 5, 10, 30 and 60 minutes of boiling exposition; it was done the comparative phytochemical examination in fine layer Chromatography in order to appreciate the main secondary metabolites that are present in each extract. To determine the toxic effects it was selected 40 Holtzmann rats with 190 +/- 10 gr. of body weight and about 2 months of age that didn't have any previous experimental treatments and belonging to the National Health Institute Lima-Peru; they were with appropriate feeding and water supply, living under environmental temperature. They were divided into 4 groups of examination to whom it was administered during 30 days by oral intake the aqueous extract of Lonchocarpus nicou roots (Barbasco) and a control group in which physiological serum was administered at dosage of 2mllkg, then it was extracted blood samples by cardiac puncture for Biochemical and Hematological exams; after that animals were killed to extract internal organs (Liver, Brain and Kidney) which were preserved in Formalin 10 per cent for Histopathological examination. It was done a descriptive and statistical analysis by means of the ANOVA of one way Test (or Kruskal-Wallis) to assess possible variations due to the effect of the extracts. The values were significant to a p<0.05. Results: It was gotten reduction of the toxic...
