Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Arch Med Res ; 47(2): 142-5, 2016 02.
Article in English | MEDLINE | ID: mdl-27133711

ABSTRACT

Glutathione S-transferases (GSTs) are a group of phase II detoxification enzymes, which catalyze the conjugation of glutathione (GSH) with carcinogens, among other xenobiotics. The GSTM3 gene is part of the GSTs gene family, and its polymorphism A/B has been associated with risk and protective effects of several cancers. This genetic variant is a deletion of 3 bp (AGG) in intron 6. Previous association studies have performed genotyping using techniques such as polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). In this study, we took advantage of the TaqMan(®) probes features and developed a reliable, faster, more simple and economic method to identify the 3-bp deletion. Our allelic discrimination method was able to distinguish between homozygous A/A, heterozygous A/B and homozygous B/B samples, as shown by TaqMan(®) based real-time PCR. Results were validated by Sanger Sequencing. In conclusion, we developed a specific and rapid method to detect the 3-bp deletion from the GSTM3 A/B polymorphism.


Subject(s)
Glutathione Transferase/genetics , Alleles , Amplified Fragment Length Polymorphism Analysis , DNA Probes , Genotype , Heterozygote , Homozygote , Humans , Introns , Isoenzymes/genetics , Polymorphism, Genetic , Real-Time Polymerase Chain Reaction , Sequence Deletion
SELECTION OF CITATIONS
SEARCH DETAIL
...