Neurons in the third abdominal ganglion of the early postnatal crayfish: a quantitative and ultrastructural study.

Ultrastructural data on the third abdominal ganglion of the crayfish was heretofore only available for adult individuals. The fine structure of neurons in the adult that are involved in the escape response has been described in detail, but no similar data existed for the postnatal individual. An increase in the number of neurons in the third abdominal ganglion during postnatal stages had been reported, which suggested that several changes in the features of neurons may occur. Here we describe the general anatomy and ultrastructure of the early postnatal third abdominal ganglion, with emphasis on neurons, and we compare their characteristics to those of the adult. Abdominal ganglia of 56 crayfish of 0, 8, 10, 18, 25, 50, 110, and 150 postnatal days were processed under cacodylate buffered aldehyde fixatives, osmicated, embedded in plastic, sectioned, and examined by light and electron microscopy. The anatomy of postnatal ganglia is homologous to the anatomy of the adult ganglia except that the perineurium is not developed in postnatals. The area of neurons within the postnatal ganglion shows no stratification, but neurons are grouped in nuclei according to their size. Neurons constitute a homogeneous population in different stages of maturity, as revealed particularly by the ultrastructure of the nucleolus. Postnatal development is evident in the perineurium, which may provide structural support to the ganglion.

Glial cells of the crayfish and their relationships with neurons. An ultrastructural study.

1. Glial cells of the crayfish abdominal ganglia have been studied by transmission electron microscopy. Special attention is paid to the interrelationships between neurons and glial cells. Covers and hemocyte-related elements have also been considered. 2. Glial cells are identified by a common ultrastructure and close relationships with neurons. Four glial classes are considered, depending on their morphology, the compartment of neurons they ensheathe and neuron-glia interface. 3. Four ultrastructural classes of neurons are proposed. They differ in geometry and ultrastructure, as well as in glial covers (complexity and evaginations into the neuron somata). The morphology and organization of glial covers is specific for the neuron type they ensheathe. Specific glial covers do not differ in glia-glia communicatory structures. 4. The morphological and metabolical compartments of neurons are separated from the extracellular matrix or blood by specific glial systems. A system of two cells is interposed between neuron somata and hemolymph or the extracellular matrix. 5. Glial processes are crossed by membraneous tubular systems, at neuron perikarya and axons. Frequent gap junctions of varying area, density and number of IMP are found in the covers of neuron somata. 6. Neuron-glia interface bears numerous communicatory structures for both ionic and macromolecular exchange. They include junctions and transient modifications of membranes. Some of them suggest active transport mechanisms. 7. Modified endocytotic mechanisms seem to be responsible for the glia-to-neuron transfer of macromolecules as well as for the neuron-to-glia transfer of lamellar bodies. 8. The neuropil is divided into glomeruli (electrical or chemical) by glial processes and the trabeculae of the extracellular dense matrix. Neuron-glia membrane appositions have been found in electrical glomeruli. In chemical glomeruli, dense cored vesicles can release their content at neuron-neuron or neuron-glia intercellular cleft, at non-synaptic loci. 9. Neurons of type II contain peripheral complex Golgi systems, associated to subsurface cisternae and neuron-glia gap junctions, suggesting a cooperation of glial cells in specific macromolecular synthesis.

Morphological aspects of the ectopic granule-like cellular populations in the albino rat hippocampal formation: a Golgi study.

Rat hippocampal formation was examined by the Golgi impregnation method. Three different ectopic granule-like populations of cells were differentiated: (1) Ectopic granule-like cells of the regio inferior, located in the stratum radiatum; one or two dendrites arose from the cell body and ran towards either the molecular layer of the fascia dentata or the stratum lacunosum-moleculare of the hippocampus, where they branched into secondary and tertiary dendrites. (2) Ectopic granule-like cells of the hilar region; this cell population showed bipolar and monopolar types of dendritic tree. Unipolar cells had dendrites oriented towards the granular layer where they branched profusely. Dendritic arborisation of bipolar cells was confined to the hilus. (3) Ectopic granule-like cells of the molecular layer; they showed several structural appearances depending on their location within the layer. Axonal tracts of ectopic granule-like cells gave rise to numerous collaterals; the main branch ran to the CA4 and CA3 hippocampal subregions. Several 'en passant' and mossy-like boutons were shown along this path.