ABSTRACT
OBJECTIVES: To analyse the relationships between the frequency of ectopic localizations of Schistosoma haematobium and S. mansoni eggs. METHODS: Studies were conducted in 11 villages in north Cameroon, around Bessoum, a village where an epidemic of bloody diarrhoea caused by S. mansoni occurred in 1997. RESULTS: The results revealed infection prevalence rates of 70.5% for S. haematobium and 30.8% for S. mansoni. Interestingly, S. mansoni eggs were found in 14.5% of the urine samples and S. haematobium eggs in 3% of the stool samples. These ectopic eliminations of schistosome eggs resulted from sexual interactions between the two species of schistosomes, and from a spill-over of high infection loads. The clinical study showed that the morbidity was lower in individuals with mixed infections and high loads of S. haematobium than in those with S. mansoni infections only, suggesting a possible lowering effect of S. haematobium infection on S. mansoni morbidity. DISCUSSION: The results obtained in human populations are discussed in relation to the known schistosome interspecific interactions in animal models.
Subject(s)
Schistosomiasis haematobia/complications , Schistosomiasis mansoni/complications , Adolescent , Animals , Cameroon/epidemiology , Child , Child, Preschool , Feces/parasitology , Female , Health Surveys , Humans , Male , Parasite Egg Count , Prevalence , Schistosoma haematobium/isolation & purification , Schistosoma mansoni/isolation & purification , Schistosomiasis haematobia/epidemiology , Schistosomiasis haematobia/parasitology , Schistosomiasis mansoni/epidemiology , Schistosomiasis mansoni/parasitology , Snails/parasitology , Urine/parasitologyABSTRACT
Several reports have described Listeria monocytogenes strains which were nonpathogenic or weakly pathogenic, but little is known about these low-virulence strains. We found that 9 field L. monocytogenes strains were hypovirulent and 17 were avirulent, based on the number of mice contaminated and the colonization of their spleens after subcutaneous inoculation. All these strains possessed the known virulence genes. We have now assessed the low virulence of these strains in other assays before determining how they differ from virulent strains. We have shown that the low-virulence strains exhibited a phenotypic stability and were not a mixture of virulent and avirulent bacteria. They did not recover virulence after many passages in mice and colonized the spleens of mice more poorly than virulent strains after i.v. inoculation. Their lethal capacities, determined by 50% lethal dose (LD(50)), were lower than those of virulent strains. Like Listeria innocua, 14 of 17 avirulent strains had no LD(50) and were eliminated by the lymph nodes after subcutaneous inoculation. The virulent, hypovirulent, and avirulent strains were always significantly different, whatever the tests of virulence used, confirming the importance of these low-virulence field strains in identifying the proteins involved in virulence.
