The immune response in canine and human leishmaniasis and how this influences the diagnosis- a review and assessment of recent research.

Leishmaniasis is a widespread but still underdiagnosed parasitic disease that affects both humans and animals. There are at least 20 pathogenic species of Leishmania, most of them being zoonotic. The diagnosis of leishmaniasis remains a major challenge, with an important role being played by the species of parasites involved, the genetic background, the immunocompetence of the host. This paper brings to the fore the sensitivity of the balance in canine and human leishmaniasis and addresses the importance of the host's immune response in establishing a correct diagnosis, especially in certain cases of asymptomatic leishmaniasis, or in the situation the host is immunosuppressed or acquired leishmaniasis through vertical transmission. The methods considered as a reference in the diagnosis of leishmaniasis no longer present certainty, the diagnosis being influenced mostly by the immune response of the host, which differs according to the presence of other associated diseases or even according to the breed in dogs. Consequently, the diagnosis and surveillance of leishmaniasis cases remains an open topic, requiring new diagnostic methods adapted to the immunological state of the host.

Septicemic Outbreak in A Rainbow Trout Intensive Aquaculture System: Clinical Finds, Etiological Agents, and Predisposing Factors.

On the 23rd of September 2022, a small intensive aquaculture unit populated with rainbow trout (Oncorhynchus mykiss) reported increased mortality in adults and juvenile fish. The unit comprised 12 enclosed concrete basins with a capacity of ten cubic meters of water, populated with 150 kg of fish each. Fish were subjected to a clinical examination on the site, after which whole fish were harvested for a bacteriological and histopathological examination. Water quality parameters were examined using classic biochemical methods and Fourier Transform Infrared Spectroscopy in order to find out whether the environment in which the fish live is also a predisposing factor that could facilitate different pathogens and induce a state of disease in the fish. Real-time PCR was performed on strains of Aeromonas spp. sampled from the fish to accurately identify the pathogen species. The goal was to accurately identify the problems and predisposing factors that lead to disease outbreaks.

The Risk of Emerging of Dengue Fever in Romania, in the Context of Global Warming.

(1) Background: Few studies to date have assessed the influences induced by climate change on the spatial distribution and population abundance of Aedes albopictus using the latest climate scenarios. In this study, we updated the current distribution of Ae. albopictus mosquitoes and evaluated the changes in their distribution under future climate conditions, as well as the risk of dengue virus emergence in Romania. (2) Methods: Under the two scenarios: High scenario (HS) when no drastic measures to reduce the effects of global warming will be taken, or they are not effective and low scenario (LS) when very stringent greenhouse control measures will be implemented. (3) Results: The results estimate an increase in temperatures in Romania of up to 2.6 °C in HS and up to 0.4 °C in LS, with an increase in the period of virus replication within the vector from June to October in HS and from May to September in LS. Moreover, in 2022, Ae. albopictus was reported in a new county, where it was not identified at the last monitoring in 2020. (4) Conclusions: The rapid spread of this invasive species and the need to implement monitoring and control programs for the Aedes population in Romania are emphasized.

Strategies for the Diagnosis of Granulocytic Anaplasmosis in Two Naturally Infected Dogs.

This study describes granulocytic anaplasmosis in two dogs naturally infected with Anaplasma phagocytophilum. The 3-year-old dogs (male and female) came from the same household and were referred to the Faculty of Veterinary Medicine in Iasi for blood donation. They were subject to standard routine tests: haematology blood test, blood smear, and serological tests (VETSCAN® FLEX4 and IDEXX SNAP 4Dx Plus). The female dog had no medical problems, while the male dog experienced joint pain. The blood smear was negative for tick-borne pathogens, and the haematology findings indicated thrombocytopenia in both dogs, with the male dog also displaying eosinophilia. The two dogs were mildly positive in the ELISA tests for the detection of Anaplasma spp. antibodies; therefore, the blood samples were tested using the qRT-PCR method for Anaplasma platys and Anaplasma phagocytophilum. The qRT-PCR result was negative for A. platys, but it was positive for A. phagocytophilum. The treatment consisted of the administration of doxycycline for 28 days. In conclusion, the high number of cases with non-specific clinical signs, the different sensitivity and specificity of the immunochromatographic serological tests, as well as the possibility of confusing the morula during the cytological examination, make the molecular test mandatory for precise diagnosis.

Occurrence of Babesia Species and Co-Infection with Hepatozoon canis in Symptomatic Dogs and in Their Ticks in Eastern Romania.

Although the distribution of Babesia spp. and Hepatozoon canis is well known in Romania, there is still a marked lack of information in many places of the country. This study aimed to investigate the occurrence of these haemoparasites in symptomatic dogs and in their ticks in Iasi, eastern Romania. Ninety owned dogs were subjected to clinical examination at the Faculty of Veterinary Medicine of Iasi and all detectable ticks (58 ticks from 15 dogs) were collected. Additionally, 124 ticks collected from the coat of other dogs (no. = 23) were included. Three Babesia species were found in dogs: Babesia canis (94.4%), Babesia vogeli (3.3%), and Babesia rossi (2.2%). All the dogs resulted negative for H. canis. The ticks were identified as follows: Ixodes ricinus (64%), Dermacentor reticulatus (33%), and Rhipicephalus sanguineus group (3%). B. canis (Minimum Infection Rate; MIR = 81%), B. vogeli (MIR = 3%), and Babesia microti-like piroplasm (MIR = 1%) were found in ticks. Moreover, 15 ticks were positive for H. canis, 6 were co-infected with B. canis, and 1 with B. microti-like piroplasm. This is the first molecular identification of B. rossi in two symptomatic dogs from Romania, although further studies are needed to investigate the vector competence of other ticks from Europe.

[Study of the nutritional status and eating habits on a group of patients in primary health care]. / Studiul starii de nutritie si a comportamentului alimentar a unui lot de pacienti din cadrul medicinii primare.

AIMS: Evaluation of medical and biological status of the study group; analyzing the nutritional status and the way of eating by food investigation; and evaluation of the knowledge about eating behavior and the role of physical activity in health. MATERIAL AND METHODS: The study was conducted from 2010-2011 on a sample of 375 patients aged 18-74, patients within a family medicine cabinet in our town. The questionnaire included demographic data (age, education, social, financial situation, the belonging religious) and questions about nutrition on food groups and questions about rational knowledge about nutrition, eating behavior and the role of physical activity. RESULTS: In the study group by gender distribution was 225 women (60%) and 150 men (40%) with a ratio F/M = 1.5/1 Evaluation of the nutritional status and somatometric exam revealed the following issues: 105 cases (28%) with, 103 cases (27.46%), 52 cases (13.86%) 72 (19.2%) and 43 cases (11.46%). Most patients were overweight normo and 27.46% respectively 28% and is followed by those with grade II obesity (19.2%), obesity grade I (13.86%) and final grade III obesity (11.46%).The food investigation on food groups showed no significant differences between the two years of study configuring same eating behavior. CONCLUSIONS: Metabolic and nutritional diseases became increasingly aggressive in recent decades and obesity is a widespread disease in the population with a multifactorial etiology and with a chronic evolution. The family doctor been in the forefront of the health system can contribute to increasing the quality of life that heis monitoring the implementation of educational programs related to food and eating behavior.

[Assessment of the effect of meteorological factors on the health of a group of patients in the city of Iasi]. / Evaluarea influentei variatiilor factorilor meteorologici asupra starii de sanatate a unui lot de pacienti din municipiul Iasi.

UNLABELLED: Analyzing the meteorological factors influence perception on state of health and evaluation of the awareness of how they act. MATERIAL AND METHODS: The study was carried out between 2010-2011 on a sample of 75 patients from of a cabinet of family medicine in the city of Iasi. The lot included randomly selected persons with age between 18-74 years. They answered a questionnaire with 25 items. The questionnaire Included demographic date (age, education, social, financial situation, the belonging religious) and questions on personal perception and evaluation of the influence of climate and weather conditions on individual state of health, degree to promote beneficial climatic factors in maintaining health and quality of life. RESULTS: The istribution of Cases in function of perception implication in meteorological factors in health reveals the following aspects: they showed significantly morecases meteoro-sensibili from urban areas (45.3%) and meteoro dependentecases from rural areas (10.7%) in our group has revealed several casesmeteorosensibile both females (36%) and the male ones (14.7%) Distribution in the study group depending on the class of diseases revealed predominant rheumatic diseases (36%) and heart disease (33.3%), haematological (20%) mental illness (14.7%) digestive (12%),respiratory diseases and neurological (10.7%). CONCLUSIONS: Meteorological facts does not represent etiological but the favoring or triggering factors in some pathological conditions. Important weather newsletters in informing and educating patients about the risks of meteorological sesnsibili requires that a necessity for maintaining health andquality of life.