Gestational Age-Specific Markers Associated with Postnatal Intervention in Fetal Suspicion of Coarctation of the Aorta.

OBJECTIVE: Fetal diagnosis of coarctation of the aorta (CoA) is currently associated with a high false-positive rate. Many predictive markers may be gestational age (GA)-specific. We sought to establish GA-specific traditional and speckle-tracking fetal echocardiography (STE) markers predictive of true CoA in neonates with prenatal suspicion. STUDY DESIGN: This is a retrospective case-control study. We compared the fetal ventricular and arch dimensions, as well as the deformation parameters by STE, of infants who required a postnatal intervention for their CoA with those who did not. Cohort was stratified based on GA before or after 30 weeks. Data extractors were masked to the outcome. The first fetal echocardiogram available was used. RESULTS: Seventy-five newborns with a fetal echocardiography performed between October 2013 and May 2022 for an antenatal suspicion of CoA were included, of which 59 (79%) had an aortic arch with nonsignificant obstruction upon ductal closure, and 16 (21%) underwent a neonatal intervention for a confirmed CoA. Before 30 weeks' GA, the right ventricular to left ventricular (RV/LV) end-diastolic width and end-diastolic area (EDA) ratios were most associated with postnatal CoA confirmation (area under the curve [AUCs] = 0.96 and 0.92). After 30 weeks' GA, the RV/LV end-diastolic width ratio (AUC = 0.95), the Z-score for the ascending aorta (AUC = 0.93), and the LV end-diastolic width Z-score (AUC = 0.91) performed the best. A decreased RV peak longitudinal strain was observed in those who developed true CoA and performed well by receiver operating characteristic analysis after 30 weeks (AUC = 0.85). In the overall cohort, the RV/LV EDA ratio was the most sensitive predictor of CoA and identified all cases with CoA. Indeed, a cutoff > 1.24 had a specificity of 69.5% and a sensitivity of 100% (receiver operating characteristic curve with an AUC of 0.88). CONCLUSION: We outlined sensitive and specific fetal markers associated with postnatal CoA based on GA at suspicion. KEY POINTS: · Fetal ventricular disproportion predicts postnatal coarctation.. · A decreased right ventircular contraction was observed in those with coarctation.. · Fetal markers differ based on gestational age at fetal evaluation..

Distinct antibody clones detect PD-1 checkpoint expression and block PD-L1 interactions on live murine melanoma cells.

Monoclonal antibodies (abs) targeting the programmed cell death 1 (PD-1) immune checkpoint pathway have revolutionized tumor therapy. Because T-cell-directed PD-1 blockade boosts tumor immunity, anti-PD-1 abs have been developed for examining T-cell-PD-1 functions. More recently, PD-1 expression has also been reported directly on cancer cells of various etiology, including in melanoma. Nevertheless, there is a paucity of studies validating anti-PD-1 ab clone utility in specific assay types for characterizing tumor cell-intrinsic PD-1. Here, we demonstrate reactivity of several anti-murine PD-1 ab clones and recombinant PD-L1 with live B16-F10 melanoma cells and YUMM lines using multiple independent methodologies, positive and negative PD-1-specific controls, including PD-1-overexpressing and PD-1 knockout cells. Flow cytometric analyses with two separate anti-PD-1 ab clones, 29F.1A12 and RMP1-30, revealed PD-1 surface protein expression on live murine melanoma cells, which was corroborated by marked enrichment in PD-1 gene (Pdcd1) expression. Immunoblotting, immunoprecipitation, and mass spectrometric sequencing confirmed PD-1 protein expression by B16-F10 cells. Recombinant PD-L1 also recognized melanoma cell-expressed PD-1, the blockade of which by 29F.1A12 fully abrogated PD-1:PD-L1 binding. Together, our data provides multiple lines of evidence establishing PD-1 expression by live murine melanoma cells and validates ab clones and assay systems for tumor cell-directed PD-1 pathway investigations.

[Deliberate self-harm (DSH). Between constraint and relief]. / Autolesiones deliberadas. Entre la restricción y el alivio.

This article is the product of the research work done on "Deliberate self-harm -DSH-: a constraint way to open to the world?" in which this Deliberate Self Harm was explored as from the phenomenological psychopathology and the suicidology theoretical approach, such research being done on the subjective and reflective narrations of people undergoing such problems, coming from different sources. Some discourse patterns are exposed that may be considered of great usefulness for the identification, the treatment -or postvention-, as well as for the prevention of the problem at issue. As an annex, a Survey of Self-harm is presented made for data collection and the planning of actions in communitarian interventions with adolescents form different places in Argentina, in conjunction with the Argentinean Professional Team of the Argentinean Association for Suicide Prevention.

Autolesiones deliberadas. Entre la restricción y el alivio / [Deliberate self-harm (DSH). Between constraint and relief].

This article is the product of the research work done on "Deliberate self-harm -DSH-: a constraint way to open to the world?" in which this Deliberate Self Harm was explored as from the phenomenological psychopathology and the suicidology theoretical approach, such research being done on the subjective and reflective narrations of people undergoing such problems, coming from different sources. Some discourse patterns are exposed that may be considered of great usefulness for the identification, the treatment -or postvention-, as well as for the prevention of the problem at issue. As an annex, a Survey of Self-harm is presented made for data collection and the planning of actions in communitarian interventions with adolescents form different places in Argentina, in conjunction with the Argentinean Professional Team of the Argentinean Association for Suicide Prevention.

Autolesiones deliberadas. Entre la restricción y el alivio. / [Deliberate self-harm (DSH). Between constraint and relief].

This article is the product of the research work done on "Deliberate self-harm -DSH-: a constraint way to open to the world?" in which this Deliberate Self Harm was explored as from the phenomenological psychopathology and the suicidology theoretical approach, such research being done on the subjective and reflective narrations of people undergoing such problems, coming from different sources. Some discourse patterns are exposed that may be considered of great usefulness for the identification, the treatment -or postvention-, as well as for the prevention of the problem at issue. As an annex, a Survey of Self-harm is presented made for data collection and the planning of actions in communitarian interventions with adolescents form different places in Argentina, in conjunction with the Argentinean Professional Team of the Argentinean Association for Suicide Prevention.