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1.
Biochimie ; 92(12): 1832-8, 2010 Dec.
Article in English | MEDLINE | ID: mdl-20713120

ABSTRACT

Human Rad51 (HsRad51), a key element of the homologous recombination repair pathway, is related to the resistance of cancer cells to chemo- and radio-therapies. This protein is thus a good target for the development of anti-cancer treatments. We have searched for new inhibitors directed against HsRad51 using the Systematic Evolution of Ligands by EXponential enrichment (SELEX) approach. We have selected three aptamers displaying strong effects on strand exchange activity. Analysis by circular dichroism shows that they are highly structured DNA molecules. Our results also show that they affect the first step of the strand exchange reaction by promoting the dissociation of DNA from the ATP/HsRad51/DNA complex. Moreover, these inhibitors bind only weakly to RecA, a prokaryotic ortholog of HsRad51. Both the specificity and the efficiency of their inhibition of recombinase activity offer an analytical tool based on molecular recognition and the prospect of developing new therapeutic agents.


Subject(s)
Aptamers, Nucleotide/metabolism , DNA/metabolism , Rad51 Recombinase/metabolism , Recombination, Genetic , Adenosine Triphosphate/metabolism , Aptamers, Nucleotide/chemistry , Aptamers, Nucleotide/genetics , Binding, Competitive , Circular Dichroism , DNA/genetics , Humans , Kinetics , Nucleic Acid Conformation , Oligonucleotides/chemistry , Oligonucleotides/genetics , Oligonucleotides/metabolism , Principal Component Analysis , Protein Binding , Rad51 Recombinase/genetics , Rec A Recombinases/genetics , Rec A Recombinases/metabolism , SELEX Aptamer Technique , Spectrophotometry
2.
Genes Cells ; 13(5): 471-81, 2008 May.
Article in English | MEDLINE | ID: mdl-18429819

ABSTRACT

Human Rad51 is a key element of recombinational DNA repair and is related to the resistance of cancer cells to chemo- and radiotherapies. The protein is thus a potential target of anti-cancer treatment. The crystallographic analysis shows that the BRC-motif of the BRCA2 tumor suppressor is in contact with the subunit-subunit interface of Rad51 and could thus prevent filament formation of Rad51. However, biochemical analysis indicates that a BRC-motif peptide of 69 amino acids preferentially binds to the N-terminal part of Rad51. We show experimentally that a short peptide of 28 amino acids derived from the BRC4 motif binds to the subunit-subunit interface and dissociates its filament, both in the presence and absence of DNA, certainly by binding to dissociated monomers. The inhibition is efficient and specific for Rad51: the peptide does not even interact with Rad51 homologs or prevent their interaction with DNA. Neither the N-terminal nor the C-terminal half of the peptide interacts with human Rad51, indicating that both parts are involved in the interaction, as expected from the crystal structure. These results suggest the possibility of developing inhibitors of human Rad51 based on this peptide.


Subject(s)
BRCA2 Protein/chemistry , BRCA2 Protein/metabolism , Peptides/pharmacology , Rad51 Recombinase/antagonists & inhibitors , Amino Acid Motifs , Apoptosis Regulatory Proteins , DNA/metabolism , Humans , Peptides/chemistry , Rad51 Recombinase/chemistry , Rad51 Recombinase/metabolism
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