Adhesion, integration and osteogenesis of human dental pulp stem cells on biomimetic implant surfaces combined with plasma derived products.

Dental implants are the usual therapy of choice in the dental clinic to replace a loss of natural teeth. Over recent decades there has been an important progress in the design and manufacturing of titanium implant surfaces with the goal of improving their osteointegration. In the present work, the aim was to evaluate the usefulness of hDPSCs (human dental pulp stem cells), in combination with autologous plasma components, for in vitro bone generation on biomimetic titanium dental implant materials. In this context, the combination of hDPSCs stimulated by PRGF or PRF and cultured on standard Ti6A14V and biomimetic BAS™ (Avinent Implant System) titanium surfaces were studied in order to evaluate possible enhancements in the osteoblastic differentiation process out of human mesenchymal cells, as well as bone matrix secretion on the implant surface. The results obtained in this in vitro model of osteogenesis suggested a combination of biomimetic rough titanium surfaces, such as BAS™, with autologous plasma-derived fibrin-clot membranes such as PRF and/or insoluble PRGF formulations, but not with an addition of water-soluble supplements of plasma-derived growth factors, to maximise osteoblastic cell differentiation, bone generation, anchorage and osteointegration of titanium-made dental implants.

Reacción inflamatoria asociada a infiltración cosmética facial múltiple. A propósitode un caso / Inflammatory reaction associated with multiple cosmetic facial infiltration. Report of a case

El uso de sustancias para el aumento de los tejidos blandos con fines cosméticos puede ocasionar la aparición de diferentes efectos adversos, entre los que se encuentran las reacciones inflamatorias tardías. Se presenta un caso clínico de una de estas reacciones en una paciente de 41 años de edad que había sido infiltrada 4 años antes en ambos surcos nasogenianos y glabelar con un gel de poliacrilamida y posteriormente con toxoide botulínico tipo A. Se discuten los principales aspectos clínico-patológicos de los procesos asociados a procedimientos cosméticos faciales (AU)

The use of substances for soft tissues augmentation for cosmetic purposes may produce different clinical adverse effects, including late inflammatory reactions. We report a case of a this reaction in a patient aged 41years old female, who was infiltrated 4 years ago in both paranasal furrows and glabelar area with apolyacrylamide gel and posteriorly with botulinum toxoid type A. We discuss the main aspects of clinical and pathological processes associated with facial cosmetic procedures (AU)

Efficacy of mometasone furoate microemulsion in the treatment of erosive-ulcerative oral lichen planus: pilot study.

BACKGROUND: Oral lichen planus (OLP) is a frequent immunological chronic disease, having different clinical forms: asymptomatic and symptomatic. Symptomatic OLP has been palliated with topical corticosteroids with different levels of efficacy and safety. The purpose of this pilot phase II clinical trial was to determine the efficacy of mometasone furoate microemulsion upon the symptoms and signs of erosive-ulcerative OLP. METHODS: Forty-nine patients with clinical and histologically confirmed erosive-ulcerative OLP were enrolled in this study (36 women and 13 men). Their average age was 56.4 years (from 28 to 78). The treatment consisted of 0.1% mometasone furoate microemulsion mouthwash three times a day over 30 days. Pain, erythema and ulceration were assessed after 15 and 30 days of treatment. The data was processed and statistically analysed by student's t-test for paired samples. RESULTS: Mometasone caused a statistically significant reduction in pain (3.58 vs. 0.65, P = 0.0000). Treatment significantly reduced the surface area of erythema (155.2 vs. 21.9 mm(2), P = 0.0001) and ulceration (30.7 vs. 7.3 mm(2), P = 0.0000). None of these patients suffered severe adverse effects. CONCLUSIONS: Mometasone furoate microemulsion is a safe and effective therapy in the treatment of symptomatic erosive-ulcerative OLP.

Gingivostomatitis after eating fish parasitized by Anisakis simplex: a case report.

Anisakis simplex (AS) is a nematode that may be encountered as a parasite in various kinds of seafood. Human beings may accidentally acquire AS larvae by eating raw or undercooked seafood. In addition to human parasitization (anisakiasis), this nematode can induce allergic reactions. AS-related diseases are frequent, especially in those countries with a high level of fish consumption and with traditions of eating raw or undercooked seafood. To our knowledge, this is the first report of gingivostomatitis secondary to the ingestion of fish with AS parasites.

Síndrome de Boca Ardiente en el País Vasco: estudio preliminar de 30 casos / Burning Mouth Syndrome in the Basque Country: a preliminary study of 30 cases

Objetivo: Conocer las principales características clinicopatológicas de los pacientes diagnosticados de Síndrome de Boca Ardiente (SBA) en nuestro medio. Material y Métodos: Estudio prospectivo sobre 30 pacientes con SBA, 29 mujeres y 1 hombre, con una edad media de 60,2 años (rango 37-89). Se cumplimentó un protocolo clínico previamente diseñado, se realizó una analítica sanguínea, una toma para cultivo de levaduras, medición del pH bucal y del flujo salival en reposo. Con los datos recogidos se realizó un análisis estadístico descriptivo y comparativo mediante chi cuadrado (p< 0,05). Resultados: Junto a la sensación de ardor o quemazón, un 60 por ciento de pacientes referían sequedad y un 60 por ciento disgeusia. La localización más habitual fue la lengua (66,7 por ciento). El tipo II de SBA fue el más común con el 53,3 por ciento. Un 82,9 por ciento de las mujeres eran postmenopáusicas. El 13,3 por ciento presentaban diabetes tipo II y un 6,7 por ciento déficits vitamínicos. Un 56,6 por ciento tomaba fármacos xerostomizantes. El 56,6 por ciento presentaba trastornos de ansiedad y /o depresión. Un 46,7 por ciento mostraba una deficiente higiene oral y el 44,4 por ciento de los portadores de prótesis utilizaban prótesis desajustadas. El flujo salival estaba reducido en el 50 por ciento de los pacientes. En ningun caso se produjo un crecimiento significativo de levaduras. Conclusiones: Los pacientes de SBA en nuestro medio corresponden principalmente a mujeres postmenopáusicas que muestran ardor lingual, xerostomía, disgeusia y ansiedad y/o depresión (AU)

Clinicopathological factors in early squamous cell carcinoma of the tongue and floor of the mouth, in Biscay (the Basque Country, Spain).

OBJECTIVES: A study is made of the main clinicopathological factors in squamous cell carcinoma (SCC) of the tongue and floor of the mouth in the province of Vizcaya (Biscay) (The Basque Country, Spain), and their relation to patient prognosis. DESIGN: A retrospective study was made of 40 patients with early (clinical stage I/II) SCC of the tongue and floor of the mouth. A previously designed protocol was used to record the clinical and histopathological data, which were subjected to descriptive and comparative bi- and multivariate statistical and survival analyses. RESULTS: There were 34 males and 6 females, with a mean age of 55.7 years (range 33-81). In 23 cases the SCC was located in the tongue, and in 17 cases in the floor of the mouth. The average tumor diameter was 2.6 cm; 65% of the neoplasms were ulcerated. The mean clinical course was 3.4 months, with an average tumor growth rate or velocity of 268 (Evans formula). Thirty-four patients were smokers and 33 consumed alcohol. All SCC of the floor of the mouth, and all regional recurrences, were diagnosed in smokers and drinkers. Survival at 5 years was 65%. Well differentiated SCC were diagnosed in 52.5% of cases. The average histological malignancy grade was 1.96, and was higher in males, tongue malignancies, T2 lesions, tumors with growth velocities above 200, and in recurrent neoplasms. CONCLUSIONS: Early SCC of the tongue and floor of the mouth in Vizcaya affects mainly male smokers and drinkers of alcohol under the age of 60 years. A relationship is observed between the histopathological findings (particularly invasion mode and stage) and patient prognosis.

Lesiones blancas bucales en el paciente geriátrico / White oral lesions in geriatric people

Las lesiones blancas de la cavidad bucal son frecuentes en los pacientes geriátricos. Un correcto diagnóstico y una actitud terapéutica acertada en estas enfermedades son fundamentales para poder descartar procesos considerados precancerosos e incluso cáncer oral. Las lesiones blancas más prevalentes en este grupo de pacientes son las queratosis irritativas (leucoplasia), las candidiasis y el liquen plano. En este revisión exponemos los principales datos clínicopatológicos de estas enfermedades (AU)

Periodontal regeneration of intrabony defects using resorbable membranes: determinants of the healing response. An observational clinical study.

The safety and bone-regenerative capacity of a resorbable membrane (Resolut) was evaluated by a nonrandomized prospective clinical study of patients with periodontal defects. Prior to surgical management all patients underwent scaling and root planing and were instructed on oral hygiene. The study included 18 patients (31 periodontal defects) who received surgical treatment by guided tissue regeneration (GTR) using resorbable membranes. The results were evaluated 12 months after surgery in terms of Plaque Index, bleeding index, probing depth, gingival recession, clinical attachment level, and dental mobility. The results obtained show that the use of resorbable membranes in GTR causes few complications. The mean gain in clinical attachment level (4.06 +/- 1.91 mm), with an attachment level gain of more than 3 mm in 81.2% of the defects, suggests the presence of "new attachment." This difference was clinically and statistically significant (t = 11.03, P = 0). The multivariate regression study showed that 60% of the observed variability (F = 11.48, P < 0.001) in clinical attachment level gain was accounted for by the variable's initial probing depth, the Plaque Index of the tooth subjected to GTR, and the infrabony component of the defect.

Warthin tumor. A new hypothesis concerning its development.

OBJECTIVE: Warthin tumor is the second most common benign parenchymal salivary neoplasm. The purpose of this study was to evaluate the histopathologic features of Warthin tumor to determine whether a developmental pattern could be identified. STUDY DESIGN: Seventy-nine Warthin tumors in 63 patients (62 male and 1 female; average age, 58.62 years) were examined. All cases were histopathologically classified and morphometrically analyzed with an Optomax system to measure the proportions of lymphoid tissue and cystic cavities. RESULTS: The predominantly epithelial type (21% of all cases) of Warthin tumor was associated with a mean patient age at diagnosis of 53 years, a mean evolution time of 2.8 months, an average size of 1 cm, and a mean lymphoid component of 10%. In the case of the classical type (61% of all cases), the mean patient age at diagnosis was 58 years, the mean evolution time 9 months, the average size 2.4 cm, and the mean lymphoid component 40%. For the predominantly lymphoid type (18% of all cases), these values were, respectively, 62 years, 11 months, 2.8 cm, and 74%. The mean size of the classical and lymphoid tumors (79% of the Warthin tumors) was significantly greater (p < 0.0001) than the mean size of the epithelial type (21% of the Warthin tumors). A positive correlation between tumor size and evolution time was found. CONCLUSION: Our results suggest that the Warthin tumor initially develops as an adenomatous epithelial proliferation followed by lymphocytic infiltration.

[Unicystic ameloblastoma as a clinicopathologic entity]. / Ameloblastoma uniquístico como entidad clinicopatológica.

Due to its clinic and histopathologic features, the unicystic ameloblastoma is a special kind of ameloblastoma, and it has a more favourable prognosis. The more interesting clinicopathologic aspects and the importance of a correct diagnosis are discussed in this paper, where we report two new mandibular cases of this tumor in young patients.

[Malherbe's calcifying epithelioma (pilomatrixoma) of the head and neck]. / Epithélioma calcifié de Malherbe (pilomatrixoma) de la tête et du cou.

Calcifying Epitheliomas of Malherbe (Pilomatrixomas) are benign tumors that have their origin in the hair matrix and clinically resemble a hard subcutaneous swelling. Most of these tumors are placed in the head and neck region, in our sample the Pilomatrixomas of this location were 49.6% of the total. The middle age of presentation of this tumor were 32.2 years, with two main peaks of incidence below 20 years and under 50. The most frequent locations were the auricular, cervical, ciliar, frontal, and temporal regions, with one case of multiple location. The middle size of the tumor in our sample was 1.2 cm. In this paper we review the main pathologic, clinic and therapeutic features of this lesion in the head and neck region.

[Metastatic hypernephroma in oral soft tissue]. / Hipernefroma metastásico en teijdos blandos orales.

Oral metastatic tumors are rare. A case of primary affection of oral soft tissues by metastatic hypernefroma, without radiologic evidence of bone involvement, is presented in this paper. Review of the literature reveals that the gingiva is the commonest location of metastatic hypernephroma in the oral soft tissues. The most important clinicopathology and therapeutic features are also reviewed.

[Gingival metastasis: a rare presenting form of renal adenocarcinoma]. / Metástasis gingival: forma rara de presentación del adenocarcinoma renal.

Malignant tumors metastasizing to the oral cavity are rare and account for 3.6% to 15.2% for renal adenocarcinoma. These tumors have gross features compatible with benign tumors or inflammatory hyperplasia. Due to the frequency of ulceration and infection of these metastatic tumors, surgical treatment is advocated.