ABSTRACT
BACKGROUND: Fatty acid synthase (FASN) is the key molecule for catalyzing fatty acid synthesis and have been associated with several malignant tumors. METHODS: We analyzed the expression of FASN and Ki-67, by immunohistochemistry on 29 carcinomas ex-pleomorphic adenoma (CXPAs) and 25 pleomorphic adenomas (PAs). RESULTS: Ki-67 proliferation index and FASN expression were significantly higher in patients with CXPA than patients with PA (P < 0.001). We found intense immunoreactivity for FASN in the malignant component of CXPAs, and these malignant areas also had intense nuclear immunoreactivity for Ki-67. CONCLUSIONS: The present results suggest that overexpression of FASN in CXPAs might be associated with malignant transformation of ductal epithelial cells and/or myoepithelial cells from PA. FASN associated with Ki-67 may be useful diagnostic markers for CXPA.
Subject(s)
Adenoma, Pleomorphic/diagnosis , Biomarkers, Tumor/metabolism , Carcinoma/diagnosis , Fatty Acid Synthase, Type I/metabolism , Ki-67 Antigen/metabolism , Neoplasms, Multiple Primary/diagnosis , Salivary Gland Neoplasms/diagnosis , Adenoma, Pleomorphic/genetics , Adenoma, Pleomorphic/pathology , Adolescent , Adult , Aged , Aged, 80 and over , Biomarkers, Tumor/genetics , Carcinoma/genetics , Carcinoma/pathology , Cell Transformation, Neoplastic , Fatty Acid Synthase, Type I/genetics , Female , Gene Expression , Humans , Immunohistochemistry , Ki-67 Antigen/genetics , Male , Middle Aged , Neoplasms, Multiple Primary/genetics , Salivary Gland Neoplasms/genetics , Salivary Gland Neoplasms/pathology , Young AdultABSTRACT
OBJECTIVE: The purpose of this study was to assess the efficacy and reproducibility of the cytologic diagnosis of salivary gland tumors (SGTs) using fine-needle aspiration cytology (FNAC). The study aimed to determine diagnostic accuracy, sensitivity, and specificity and to evaluate the extent of interobserver agreement. STUDY DESIGN: We retrospectively evaluated SGTs from the files of the Division of Pathology at the Clinics Hospital of São Paulo and Piracicaba Dental School between 2000 and 2006. RESULTS: We performed cytohistologic correlation in 182 SGTs. The sensitivity, specificity, positive predictive value, negative predictive value, and diagnostic accuracy were 94%, 100%, 100%, 100%, and 99%, respectively. The interobserver cytologic reproducibility showed significant statistical concordance (P < .0001). CONCLUSIONS: FNAC is an effective tool for performing a reliable preoperative diagnosis in SGTs and shows high diagnostic accuracy and consistent interobserver reproducibility. Further FNAC studies analyzing large samples of malignant SGTs and reactive salivary lesions are needed to confirm their accuracy.
