Diet with different concentrations of lychee peel flour modulates oxidative stress parameters and antioxidant activity in zebrafish.

The agri-food industry generates substantial waste, leading to significant environmental impacts. Lychee (Litchi chinensis Sonnerat), which is rich in bioactive compounds in its peel, pulp, and seeds, offers an opportunity for waste use. This study aimed to evaluate the effects of supplementing a high-carbohydrate diet with varying levels of lychee peel flour on lipid metabolism biomarkers and oxidative stress in a zebrafish (Danio rerio) model. A total of 225 zebrafish, approximately four months old, were divided into five groups: control, high-carbohydrate (HC), HC2%, HC4%, and HC6%. The study did not find significant differences in the growth performance of zebrafish in any group. However, the HC6% group exhibited a significant decrease in glucose and triglyceride levels compared with the HC group. Furthermore, this group showed enhanced activities of the antioxidant enzymes catalase (CAT) and superoxide dismutase (SOD), along with reduced levels of malondialdehyde (MDA). Increased antioxidant activity was also evidenced by DPPH-, ABTS+, and ß-carotene/Linoleic acid assays in the HC6% group. A positive correlation was identified between SOD/CAT activity and in vitro antioxidant assays. These findings suggest that dietary supplementation with 6% lychee peel flour can significantly modulate glucose homeostasis, lipid metabolism, and antioxidant activity in zebrafish.

Profile identification of canicross practitioners and their dogs in Brazil / Identificação do perfil dos praticantes de canicross e seus cães no Brasil / Identificación del perfil de los practicantes de canicross y sus perros en Brasil

The study analyzed the profile of Canicross practitioners in Brazil. A semi- structured questionnaire consisting of 33 questions was used: seven related to the handler and 26 to the dogs. Results: Of the total handlers, 53.3% are women, 64% have practiced the sport for at least 5 years and 52.3% trained with a personal trainer. Of the dogs, 62.6% were females, 60% were between one and four years old and the Mixed Breeds were the majority with 34.6%; as for the health of the animals, 80% of the handlers stated that they did not carry out veterinary monitoring aiming at the Canicross. 6.6% of the animals had sports-related injuries and 80% of these cases received veterinary treatment. It was clear that handlers know strategies to prevent and / or treat injuries caused by the sport, however this is not reflected in dogs. The unprecedented nature of the work will help handlers, veterinarians and sports lovers to draw up protocols so that dogs can practice Canicross safely.

O estudo analisou o perfil dos praticantes de Canicross no Brasil. Foi utili- zado um questionário semiestruturado composto por 33 questões, sendo sete relacionadas ao condutor e 26 aos cães. Resultados: Do total de manipuladores, 53,3% são mulheres, 64% praticam o esporte há pelo menos 5 anos e 52,3% treinam com personal trainer. Dos cães, 62,6% eram fêmeas, 60% tinham entre um e quatro anos e os sem raça definida eram a maioria com 34,6%; quanto à saúde dos animais, 80% dos tratadores afirmaram não realizar acompanhamento veterinário visando o Canicross. 6,6% dos animais tiveram lesões esportivas e 80% desses casos receberam tratamento veterinário. Ficou claro que os tratadores conhecem estratégias para prevenir e/ou tratar lesões causadas pelo esporte, porém isso não se reflete nos cães. O caráter inédito do trabalho ajudará tratadores, veterinários e esportistas a traçarem protocolos para que os cães possam praticar Cani- cross com segurança.

El estudio analizó el perfil de los practicantes de Canicross en Brasil. Se utilizó un cuestionario semiestructurado compuesto por 33 preguntas, siendo siete relaci- onadas con el conductor y 26 con los perros. Resultados: Del total de manejadores, 53,3% son mu-lheres, 64% practican el deporte hace por lo menos 5 años y 52,3% entrenan con trai-ner personal. De los perros, 62,6% eran hembras, 60% tenían entre uno y cuatro años y los sin raza definida eran la mayoría con 34,6%; en cuanto a la salud de los animales, 80% de los manejadores afirmaron no realizar acompañamiento veterinario con vistas al Canicross. El 6,6% de los animales presentaron lesiones deportivas y el 80% de estos casos recibieron tratamiento veterinario. Quedó claro que los cuidadores conocen estra- tegias para prevenir y/o tratar lesiones causadas por el deporte, sin embargo esto no se refleja en los perros. El carácter inédito del trabajo aju-dará a cuidadores, veterinarios y deportistas a trazar protocolos para que los perros puedan practicar Canicross con segu- ridad.

Cannibalism rate and mLeptin expression are influenced by photoperiod and diets in Piracanjuba, Brycon orbignyanus (Valenciennes, 1850) larvae.

Piracanjuba (Brycon orbignyanus) is a species with great productive potential, and during its larval phase, it presents intense cannibal activity. The photoperiod and diet are primary feed behaviour and cannibalism modulators to fishes. This experiment aimed to verify the effect of different photoperiods and diets in Piracanjuba larviculture. Larvae were kept under different photoperiods - 12 h light: 12 h dark (12 L: 12D); 24 h light:00 h dark (24hL: 00D) - Larvae were fed with Artemia nauplii and a formulated micro-diet in a factorial scheme for 10 days, and at the end of the experimental period, the influences of the treatments on performance and quantitative expression of mLeptin and mBmall1 were evaluated. In order to quantify the expression of mLeptin and mBmall1, qPCR adopting ß-actin and Elongation Factor 1 as endogenous genes was used. The primers for all the analysed transcripts were obtained through multiple sequences alignments of different fish species. It was observed that the diet and photoperiod influence the performance of Piracanjuba (B. orbignyanus) larvae in the initial phase of larviculture. Feeding with artemia nauplii and the photoperiod of 24 L:00D reduce cannibalism rates in intensive Piracanjuba larviculture. The results on the rate of cannibalism, rate of survival and the relative expression of mLeptin are related to the survival rate of the larvae, and it is inversely proportional to the cannibalism rate. The expression levels of mBmall1 showed a correlation with the final weight of the larvae. Piracanjuba Larvae under a photoperiod of 24 light and fed Artemia nauplii showed more significant levels of mLeptin expression.

A ingestão do anti-inflamatório não esteroide naproxeno afeta parâmetros neuromusculares e metabólicos? / Does the ingestion of naproxen affect neuromuscular and metabolic parameters?

O naproxeno, assim como outros anti-inflamatórios não esteroides (AINEs), está entre os medicamentos mais prescritos no mundo. O objetivo do presente estudo é analisar o efeito da ingestão de naproxeno em parâmetros neuromusculares e determinar seu efeito no dano muscular por meio do uso do marcador lactato. Metodologicamente, foi conduzido um estudo cruzado randomizado, duplo-cego e controlado por placebo em 11 homens treinados em resistência, que realizaram uma sessão de treinamento de força após ingerir 500 mg de naproxeno e outra sessão de treinamento após ingerir um placebo. Os participantes realizaram três séries de supino horizontal com uma carga de 90% da repetição máxima (1RM) até a falha concêntrica. As variáveis de resultado incluíram número de repetições, carga de trabalho e lactato. Os resultados mostraram que há uma correlação positiva e moderada entre as variáveis somatório de repetições e carga total e entre as variáveis lactato e carga total, no grupo naproxeno. No grupo placebo, a correlação positiva e moderada deu-se entre somatório de repetições e carga total. Na análise magnitude baseada nas interferências, as variáveis se mostraram possíveis para uma probabilidade positiva ou trivial e improvável para uma probabilidade negativa. Concluiu-se no presente estudo que o uso do naproxeno como recurso ergogênico no treinamento de força reduz a percepção de fadiga, mas não tem efeito direto no dano muscular, analisado a partir do marcador lactato, logo não interfere de maneira significativa nos parâmetros neuromusculares analisados.

Naproxen, as other non-steroidal anti-inflammatory drugs (NSAIDs), features among the most widely prescribed drugs in the world. The aim of this study is to analyze the effect of naproxen intake on neuromuscular parameters and determine its effect on muscle damage through the use of the lactate marker. In terms of methodology, a randomized, double-blind, placebo-controlled crossover study was conducted on 11 resistance-trained men who underwent a strength training session after taking 500 mg of naproxen and another training session after taking a placebo. The participants performed three sets of horizontal bench presses with a load of 90% maximum repetition (1RM) until concentric failure. Result variables included number of repetitions, workload and lactate. The results showed that there is a positive and moderate correlation between the sum of repetition and total load variables and between lactate and total load variables in the naproxen group. In the placebo group, a positive and moderate correlation was observed between sum of repetitions and total load. In the magnitude analysis, based on the interferences, the variables were shown to be possible for a positive or trivial probability and unlikely for a negative probability. It was concluded that the use of naproxen as an ergogenic resource in strength training reduces the perception of fatigue but has no direct effect on muscle damage when analyzed from the lactate marker, therefore it does not significantly interfere in the analyzed neuromuscular parameters.

Características antropométricas y potencia de miembros inferiores en jugadores universitarios de rugby-7

El objetivo de este estudio fue comparar las variables antropométricas y de potencia de miembros inferiores (PMI) en una muestra de jugadores de rugby-7 de diferentes posiciones. Participaron 22 jugadores universitarios clasificados de acuerdo con las posiciones ocupadas en el terreno de juego en back y forward. Para evaluar el porcentaje de grasa, se utilizó el protocolo de 7 pliegues cutáneos de Jackson & Pollock, y para las pruebas de PMI salto vertical (CMJ), salto horizontal y salto triple. Con el afán de comparar las variables del estudio, fue adoptado el test T para muestras pareadas, siendo el p < 0,05. Los resultados indicaron una diferencia significativa entre la masa total (t = , p < 0,01), la masa libre de grasa (p < 0,05), la masa adiposa (p < 0,01) y el CMJ (p < 0,005) entre los grupos. Se observa que las características antropométricas y neuromusculares para los forwards son distintas de las de los backs, debido a la posición de juego, que requiere una configuración morfológica y física distinta.

The objective of this study was to compare the anthropometric and lower limb power (PMI) variables in a sample of rugby-7 players holding different positions. Twenty-two rugby-7 college players participated; according to the positions occupied on the playing field, they were classified in back and forward. The protocol of seven skin folds of Jackson & Pollock was used to evaluate the percentage of fat; and for the PMI tests vertical jump (CMJ), horizontal jump and triple jump. The T-test for paired samples was adopted, being p < 0.05, to compare the study variables. The results indicated a significant difference between the total mass (p < 0,01), the fat-free mass (p < 0,05), the fat mass (p < 0,01), and the CMJ (p < 0,005) between the groups. It is observed that the anthropometric demands for the forwards are different from the backs, due to the characteristic of the game position, which requires a different physical and morphological configuration.

O objetivo deste estudo foi comparar as variáveis antropométricas e de potência dos membros inferiores (PMI) em uma amostra de jogadores de rúgbi-7 de diferentes posições. Participaram 22 jogadores universitários, classificados de acordo com as posições ocupadas no campo de jogo para adiante e para trás. Para avaliar o percentual de gordura, utilizou-se o protocolo de 7 dobras cutâneas de Jackson & Pollock, e para os testes de PMI salto vertical (CMJ), salto horizontal e salto triplo. Com o objetivo de comparar as variáveis do estudo, o teste T foi adotado para amostras de dupla, sendo p <0,05. Os resultados indicaram uma diferença significativa entre a massa total ( t =, p <0,01), a massa livre de gordura (p <0,05), a massa adiposa (p <0,01) e o CMJ (p < 0,005) entre os grupos. Observa- se que as características antropométricas e neuromusculares para os atacantes são diferentes daquelas das costas, devido à posição de jogo, que requer uma configuração morfológica e física diferente.