[The histological study of adenopathies in laryngeal cancer with supraglottic localization]. / Estudio histológico de las adenopatías en el cáncer de laringe de localización supraglótica.

A histopathologic study was made to establish the prognostic importance of lymph node involvement in operable supraglottic laryngeal carcinoma. A total of 51 cancer patients underwent supraglottic horizontal laryngectomy with bilateral cervical dissection. The number of adenopathies the presence of capsule rupture and type of corresponding infiltration were significantly related to survival (p < 0.01). Prognosis was poorer in patients with more than three metastatic adenopathies and/or capsule rupture of one or more of them. Patient survival in cases with capsule rupture affecting one or more nodes was 36%, versus 33% among patients with three or more metastatic nodes. It is concluded that (a) the poor survival rate observed among these patients points to the need for more aggressive treatment protocols; and (b) cervical dissection should be performed systematically not only for therapeutic purposes but also to improve patient prognosis.

Analysis of DNA content in supraglottic epidermoid carcinoma.

DNA analysis by flow cytometry is considered to be of prognostic value in epidermoid carcinoma of the head and neck. However, few and contradictory studies have been made on laryngeal carcinomas. We studied 48 epidermoid carcinomas in patients subjected to horizontal supraglottic laryngectomy with a 5-year- followup. The technique described by Hedley for fixated and paraffin-embedded tumors was used. Thirteen tumors were excluded on the grounds of presenting variation coefficients in excess of 10. Of the 35 cases analyzed, 28 (80%) were diploid and seven (20%) aneuploid. No correlation was observed between tumor ploidy and patient survival, recurrence, or any of the histopathological variables studied.

Prognostic value of histopathological parameters in 51 supraglottic squamous cell carcinomas.

The prognosis for supraglottic squamous cell carcinomas is difficult to establish. A study of 19 histopathological parameters (14 from the primary tumor and 5 from the lymph node metastases) was undertaken in 51 supraglottic epidermoid carcinomas. All patients were subjected to supraglottic horizontal laryngectomy with bilateral functional neck dissection and postoperative radiotherapy. A discriminant analysis was performed to relate the different histopathological variables to 5-year survival. Capsule rupture and the number of metastatic lymph nodes were the only two parameters significantly related to survival (P < .01). Histopathology (in particular, lymph node metastases) proved more effective in predicting patient evolution than TNM staging.

[Comparative study of histology and flow cytometry results obtained in 2 groups of laryngeal tumors, glottic and supraglottic, in T-1 stage]. / Estudio comparativo de los resultados histológicos y de citometría de flujo obtenidos en dos grupos de tumores laríngeos, glóticos y supraglóticos, en estadio T-1.

We studied histophatological parameters and DNA content in 27 supraglottic and 37 glottic T-1 squamous cell carcinoma of the larynx. The DNA content was determined by the Hedley method in paraffin-embedded sections of formalin-fixed blocks in an EPICS Profile flow cytometer. We studied 14 histopathological parameters and only 3 were statistically different in both groups of tumors: Differentiation, infiltrative degree and mitoses. There weren't statistical differences in DNA content (DNA index and proliferation index).

Primary vulvar lymphoma presenting as a clitoral tumor.

We report a case of malignant large cleaved cell vulvar lymphoma predominantly affecting the clitoris, in a 60 year-old woman. In a review of the literature, female genital tract lymphomas of vulvar location are exceptional. Histologically, these tumors must be differentiated from pseudolymphomatous lesions. In the present case (Stage IE), response to chemotherapy was good and the patient remains asymptomatic after three years of follow-up.

[Endogenous peroxidase activity of the laryngeal epithelium: experimental study]. / Actividad peroxidásica endógena del epitelio laríngeo: estudio experimental.

Twenty vocal cords were studied. The used animals were rabbits. Beta-blockers and beta-stimulants were perfused by the jugular vein. Variations in endogenous peroxidase were observed in electron microscopy. The activity of endogenous peroxidase can be used according to our results as tracer of the autonomic innervation of the vocal cord.

[An analysis of the DNA content in a stage-T-1 epidermoid carcinoma of the vocal cord. The methodology and results]. / Análisis del contenido de DNA en el carcinoma epidermoide de cuerda vocal en estadio T-1. Metodología y resultados.

DNA content of tumor cells determined by flow cytometry has been correlated to biologic behavior and to prognosis for many solid tumors, but insufficient data has been accumulated for squamous cell carcinoma of the larynx. In order to know the predictive value of this parameter in the prognosis of T-1 vocal cord squamous cell carcinoma, we have studied 27 patients submitted to cordectomy. The patients outcome was followed for a minimum of five years or until death, DNA content was determined by Hedley method in paraffin-embedded sections of formalin-fixed blocks in an EPICS Profile flow cytometer. 24 of these tumors were diploid (89%) and 3 aneuploid (11%). We didn't find statistical correlation between ploidy and survival, local failure or someone of the pathological parameters.

[Granular cell tumor (Abrikossoff's tumor). A review and our experience]. / Tumor de células granulares (tumor de Abrikossoff). Estudio de revisión y nuestra experiencia.

The low incidence of these tumors compelled the AA., after diagnosis of one of laryngeal sitting, to a review of the theme and its literature as well, particularly under the viewpoint of its histogenesis, which has been debatable up to lately. The report is complemented with the study of immunohistochemical tests for NHK, S-100 and specific neural enolase of the specimen.

[Prognostic value of histopathological parameters in epidermoid carcinoma of the vocal cords in T-1 stage]. / Valor pronóstico de los parámetros histopatológicos en el carcinoma epidermoide de cuerda vocal en estadio T-1.

In this paper are studied macro- and microscopically, 14 histopathologic parameters belonging to 37 patients suffering from vocal cord squamous cell carcinoma, stage T-la. All patients were submitted to cordectomy and followed up during a 5 years term, as a minimum. A discriminant analysis was performed in order to establish the relation between histopathologic parameters and local failure. Only two of them were statistically significative (p < 0.01): the structural type and the differential degree of tumor cells. The histopathological study allowed the prediction of 82 percent of local recurrences.

[Olfactory neuroblastoma. A clinical and morphological study]. / Neuroblastoma olfatorio. Estudio clínico y morfológico.

A clinical and morphological study about neuroblastoma with olfactory differentiation are made. This study includes: TAC, vanimil mandelic acid urine determination, optic microscope, immunohistochemical technique, neuropeptidal, in situ hybridization, DNA analysis by flow cytometry and electronic microscope.

[Flow cytometry. Its prognostic value in cancer of the head and neck]. / Citometría de flujo. Su valor pronóstico en el cáncer de cabeza y cuello.

The authors introduce an historical review and technical information on the management of this technique. The current condition of this matter is commented on, with special interest in head and neck tumors. Very few, contradictory papers have been published on laryngeal tumors concerning the prognostic value of aneuploid tumors.

Primary omental tumor with ultrastructural features of cellular schwannoma and absence of S-100 antigen reactivity.

We report a primary well-delimited, large mesenchymal omental neoplasm which appeared in a 58 year old man without neurofibromatous traces. The histological and ultrastructural features were consistent with a Schwann cell origin. However, repeated immunohistochemical reactions failed to demonstrate protein S-100 antigen reactivity in the neoplastic cells, the latter only expressing vimentin. The differential diagnosis with hemangiopericytoma is proposed.

The autonomic innervation of the human vocal cord: neuropeptides.

Three methods have been used to study the sympathetic and parasympathetic innervation of the vocal cord: paraformaldehyde-induced fluorescence, electron microscopy, and the peroxidase-antiperoxidase technique. Each method has been successful in determining adrenergic fibers, autonomic endings, and the neuropeptides VIP and NPY, respectively. The close relationship between these neuropeptides and the autonomic endings (adrenergic and cholinergic), and the presence of the neuropeptides in the chorion of the vocal cord, around glands and vessels, gives new evidence that autonomic innervation plays a role in the functioning of this part of the larynx.

Multicentric reticulohistiocytosis.

A case of multicentric reticulohistiocytosis in a 49-year-old man associated with polymyositis is presented. The clinical features along with the light and electron microscopic studies are commented. Partial improvement of the skin lesions was achieved after treatment with azathioprine but the patient's general condition remained unchanged.