[Localization of a 1-anilinonaphthalen-8-sulfonate fluorescent probe on the surface monolayer of low density lipoproteins]. / Lokalizatsiia fluorestsentnogo zonda 1-anilinonaftalen-8-sul'fonata v poverkhnostnom monosloe lipoproteidov nizkoi plotnosti.

A decay of fluorescence probe 1-anilino-8-naphtalene sulfonate (ANS) sorbed on low density lipoproteins (LDL) surface obtained from human plasma was described. It was demonstrated that on the LDL surface the ANS probe is allocated among two pools of molecules with the time of fluorescence decay 4-7 ns and 12-16 ns. One may conclude that 75-90% of the probe is connected with lipid LDL matrix.

[Electrostatic potential on the surface of low density lipoproteins from the plasma of patients with ischemic heart disease: estimation using a fluorescent probe]. / Elektrostaticheskii potentsial poverkhnosti lipoproteidov nizkoi plotnosti plazmy krovi bol'nykh ishemicheskoi bolezn'iu serdtsa: issledovanie s pomoshch'iu fliuorestsentnogo zonda.

Fluorescent probe I-aniline-8-naphthalene sulfonate was used to evaluate the LDL surface potential values in patients with coronary artery atherosclerosis. The more negative charge was found on the LDL surface in patients with atherosclerosis of three coronary arteries as compared with that of healthy persons.

[Interaction of plasma lipoproteins and apolipoproteins with lipid bilayer membranes]. / Izuchenie vzaimodeistviia lipoproteidov plazmy krovi i apolipoproteinov s bisloinymi lipidnymi membranami.

It was shown that the interaction of lipoproteins (LP) with bilayer lipid membranes (BLM) resulted in some changes in the physical-chemical properties of the membranes. Adsorption of very low and low density lipoproteins (VLDL and LDL) at concentrations of 5-8 g protein/ml increased the surface potential difference and decreased transversal elasticity module of the bilayer. LP concentrations higher than the mentioned ones increased BLM conductance and caused instability and disruption of the membranes. The same effects were revealed for high density lipoproteins (HDL) at higher concentrations--15-20 micrograms protein/ml. The effect of apolipoproteins in the interaction of LP with BLM was investigated. It is proposed that apolipoproteins and especially apo B are the main factor which affects the nonreceptor interactions of LP with the membranes.

[Peculiarities of interactions of bilayer lipid membranes with human plasma lipoproteins from patients with ischemic heart disease]. / Osobennosti vzaimodeistviia s BLM lipoproteidov, vydelennykh iz plazmy liudei s ishemicheskoi bolezn'iu serdtsa.

It is shown that the modifying ability sequence of human plasma lipoproteins from patients with ischemic heart disease (IHD) in respect to BLM made of oxidized cholesterol is [HDL2] less than [LDL] less than [VLDL] less than [HDL3] and differs from that in healthy people which is [VLDL], [LDL] less than [HDL2] less than [HDL3]. It is suggested that changes in lipid-protein compositions of the lipoproteins in the case of IHD determined the alterations of the nonreceptor interactions of the atherogenic and antiatherogenic lipoprotein classes with the cell membranes.

[Plasma lipoproteins, modifiers of the ionic permeability of artificial lipid membranes]. / Lipoproteidy plazmy krovi--modifikatory ionnoi provodimosti iskusstvennykh lipidnykh membran.

With the help of BLM a possibility of non-receptor interaction between the blood plasma lipoproteides and isolated apoproteins immediately with the lipid part of plasmic membranes was shown. Incorporation of lipoproteides in BLM is of a cooperative character and induces cationic permeability in them. Specific interactions with the membranes of aterogenic and antiaterogenic classes of lipoproteides, the role of dimensions and surface charge of lipoproteide particles and of apoproteins and calcium ions in the course of incorporation are revealed. It is suggested that asymmetrical incorporation of a lipoproteide particle in the bilayer and a local increase of the membrane permeability for calcium results in the activation of the contractile system of the cortical layer and in the formation of endocytosis vacuole transporting the lipoproteide particles into the cell.

[Effect of human plasma lipoproteins on ionic conductivity of lipid bilayer membranes]. / Vliianie lipoproteidov plazmy krovi cheloveka na ionnuiu provodimost' bisloinykh lipidnykh membran.

An interaction was studied between various lipoproteins (low density lipoproteins--LDL, very low density lipoproteins--LVDL, subfractions of high density lipoproteins--HDL2, HDL3) and artificial bilayer lipid membranes (BLM) produced from oxydized cholesterol. The lipoproteins increased from 100 to 1000-fold the conductivity of BLM for cations Na+, K+, Ca2+, but not for anions. Critical concentrations of the lipoproteins at which their modifying effect was manifested, correlated reciprocally with the size of the lipoprotein particles; they were arranged as follows: VLDL < LDL < HDL2 < HDL3. Decrease in the pH value lowered these critical concentrations. Addition of Ca2+ into the solution decreased the critical concentrations of VLDL and LDL from 2- to 4-fold as well as of HDL2 and HDL3--from 9- to 12-fold. The electrostatic power appears to be important in interaction between the lipoproteins and bilayer lipid membranes.