ABSTRACT
In mice with Lewis lung carcinoma and melanoma B-16 administration of potentiated antibodies to tumor necrosis factor-alpha started 1 day after transplantation of a small number of tumor cells (10(6)) produced an antiblastic effect.
Subject(s)
Antibodies/therapeutic use , Antineoplastic Agents/therapeutic use , Tumor Necrosis Factor-alpha/immunology , Animals , Antibodies/immunology , Carcinoma, Lewis Lung/drug therapy , Melanoma/drug therapy , Mice , Mice, Inbred C57BLABSTRACT
Hypotensive activity of ultralow doses of antibodies to some endogenous substances involved in the regulation of vascular tone was studied on NISAG rats with hereditary stress-induced arterial hypertension. It was found that antibodies to angiotensin II and its receptor in ultralow doses markedly reduced systolic blood pressure, which was reproducible after repeated treatment. The course of peroral treatment with antibodies to endothelin and endothelial NO synthase in ultralow doses did not decrease systolic blood pressure.
Subject(s)
Antibodies/pharmacology , Antihypertensive Agents/pharmacology , Blood Pressure/drug effects , Endothelins/immunology , Nitric Oxide Synthase/immunology , Angiotensin II/immunology , Animals , Antibodies/administration & dosage , Antibodies/immunology , Antihypertensive Agents/administration & dosage , Antihypertensive Agents/immunology , Dose-Response Relationship, Drug , Male , RatsABSTRACT
Hypotensive activity of ultralow doses of antibodies to angiotensin II and its receptors was studied on adult NISAG rats with hereditary stress-induced arterial hypertension. Antibodies to C-terminal fragment of angiotensin II receptors produced the most pronounced hypotensive effect, which was reproducible after repeated administration. These antibodies decreased systolic blood pressure by 16.40 +/- 0.62 mm Hg. The most rapid hypotensive effect was produced by affinity purified antibodies to angiotensin II: 2 h after administration of these antibodies systolic blood pressure decreased by 12.80 +/- 5.49 mm Hg. Our results indicate that combination treatment with ultralow doses of antibodies to angiotensin II and its receptors hold much promise for the use in clinical practice.
Subject(s)
Angiotensin II/immunology , Antibodies/pharmacology , Antihypertensive Agents/pharmacology , Blood Pressure/drug effects , Hypertension , Receptor, Angiotensin, Type 1/immunology , Animals , Antibodies/immunology , Antihypertensive Agents/immunology , Male , RatsABSTRACT
Single and course administration of ultralow doses of antibodies gamma-interferon did not increase the incidence of cytogenetic abnormalities in bone marrow cells from BALB/c mice and produced no genotoxic effect on Drosophila melanogaster wing cells in the test of somatic mosaicism.
Subject(s)
Antibodies/toxicity , Bone Marrow Cells/drug effects , Drosophila melanogaster/drug effects , Interferon-gamma/immunology , Mutagens/toxicity , Animals , Antibodies/immunology , Bone Marrow Cells/cytology , Cells, Cultured , Dose-Response Relationship, Drug , Mice , Mice, Inbred BALB C , Mutagenicity Tests , Wings, Animal/cytology , Wings, Animal/drug effectsABSTRACT
We studied the effects of ultralow doses of anti-idiotypic antibodies to human gamma-interferon on humoral immune response in experimental animals immunized with a thymus-dependent antigen, delayed-type hypersensitivity, and phagocytic activity of neutrophils in the peritoneal exudate. This preparation stimulated the humoral immune response and activated phagocytosis, but had no effect on functional activity of T cells.
Subject(s)
Antibodies, Anti-Idiotypic/pharmacology , Antibody Formation/drug effects , Immunity, Cellular/drug effects , Interferon-gamma/immunology , Animals , Dose-Response Relationship, Drug , Female , Humans , Male , Mice , Neutrophils/drug effects , Neutrophils/immunologyABSTRACT
We studied immunotropic properties of antibodies to human interferon-gamma in homeopathic doses. The effects of these antibodies on the ratio between the counts of T helper (CD4+) and T suppressor cells (CD8+), production of interleukin-1 and interleukin-2, and functional activity of natural killer cells were evaluated. Potentiated antibodies slightly decreased the number of CD8+ cells in the spleen of experimental animals. After the addition to cultured human mononuclear cells the preparation produced a moderate comitogenic effect, stimulated proliferation of T and B lymphocytes, increased spontaneous and induced production of interleukin-1, and enhanced functional activity of natural killer cells (under certain conditions).
Subject(s)
Antibodies/pharmacology , Immunologic Factors/pharmacology , Interferon-gamma/immunology , Adult , Animals , CD4-Positive T-Lymphocytes/drug effects , CD4-Positive T-Lymphocytes/metabolism , CD8-Positive T-Lymphocytes/drug effects , CD8-Positive T-Lymphocytes/metabolism , Cells, Cultured , Humans , Interleukin-1/metabolism , Interleukin-2/metabolism , Killer Cells, Natural/drug effects , Killer Cells, Natural/metabolism , Leukocytes, Mononuclear/drug effects , Leukocytes, Mononuclear/metabolism , Male , Mice , Young AdultABSTRACT
Morphological and morphometric assays showed that administration of antibodies to the prostate-specific antigen in ultralow doses for 1.5 months delayed the development of atrophic and sclerotic processes in rats with chronic aseptic prostatitis. The concentration of zinc ions playing an important role in binding of androgens increased in the prostate of rats receiving the preparation. Studies of copulatory behavior showed that male rats with chronic prostatitis receiving antibodies to the prostate-specific antigen displayed increased sexual activity compared to control and intact animals.
Subject(s)
Antibodies/pharmacology , Prostate-Specific Antigen/immunology , Prostate/drug effects , Animals , Dose-Response Relationship, Drug , Male , Prostate/pathology , Prostatitis , Rats , Sexual Behavior, Animal/drug effectsABSTRACT
A blind, placebo-controlled, randomized trial showed clinical effectiveness of the preparation Impaza containing antibodies to endothelial NO synthase in ultralow doses. Impaza surpassed placebo, but was less potent than sildenafil in the ability to improve erectile function and other parameters characterizing sexual activity of men. Safety of Impaza was greater than that of other test preparations.
Subject(s)
Antibodies/therapeutic use , Erectile Dysfunction/drug therapy , Piperazines/therapeutic use , Sulfones/therapeutic use , Adult , Aged , Antibodies/adverse effects , Double-Blind Method , Humans , Male , Middle Aged , Piperazines/adverse effects , Purines/adverse effects , Purines/therapeutic use , Sildenafil Citrate , Sulfones/adverse effects , Treatment Outcome , Young AdultABSTRACT
Single and repeated administration of ultralow doses of antibodies to erythropoietin did not increase the count of aberrant metaphases in bone marrow cells of BALB/c mice and were not genotoxic for Drosophila melanogaster wing cells in the test of somatic mosaicism.
