Evaluation of cytotoxic effects of fungal origin nanosilver particles on oral cancer cell lines: An in vitro study.

Background: Oral cancer is often associated with poor prognosis and it is found that conventional treatment options cause severe side effects, adjacent tissue disfigurement, and loss of function. Recently, silver nanoparticles (AgNPs) paved their path for cancer treatment. Aim: This study aimed to investigate cytotoxic effects of fungal procured AgNPs on oral squamous cell carcinoma (SCC-9) cell line using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Methodology: The silver nanoparticles were biosynthesized using the fungi Fusarium semitectum. Cell lines were cultured in a 1:1 ratio of Dulbecco's Modified Eagle's medium and Ham's F12 medium and subcultured in a T-75 cm2 flask. Cell count was adjusted to 1 × 105 cells/ml; 50,000 cells/well were seeded into a 96 well plate and incubated at 37°C, for 24 h in 5% CO2 humidified conditions. AgNPs (1.75-50 µl/ml) were added to the plates and further incubated at 37°C for another 24 h. Medium containing cells without AgNPs were used as a control group. Later, 20 µl of MTT was added to each well and incubated for 6 h at above-mentioned conditions. About 0.1 mL of Dulbecco's Modified Eagle's Medium solution was added to each well to solubilize formazan. The absorbance was measured using a Tecan reader at 540 nm. The experiment was repeated thrice independently. The percentage (%) inhibition of growth and the AgNP's concentration that prevents the cell growth by 50% (IC50) were determined. Results: Significant dose-dependent inhibition of the growth of SCC-9 cell lines was seen and IC50 was found at 12 µl/ml concentration of AgNPs. Conclusion: Biosynthesized AgNPs of fungal origin exhibit effective anticancer properties against the SCC-9 cell line.

Efficiency of an Integrated Apex Locator in Determining Working Length in Various Irrigating Solutions: An In Vivo Study.

OBJECTIVES: The objective of this in vivo study was to compare and evaluate the accuracy of VDW Gold (VDW, Munich, Germany) integrated apex locator (IAL) and Root ZX Mini (J. Morita Corp., Kyoto, Japan) in two different irrigating solutions. MATERIALS AND METHODS: Forty patients who required extraction were included in the study. Following local anesthesia, access opening was performed under rubber dam isolation. Initial negotiation of canal was performed using size 10 K-Files (M-Access, Dentsply Maillefer, Ballaigues, Switzerland). Cervical preparation was carried out using Rotary ProTaper SX files (Dentsply Maillefer, Ballaigues, Switzerland) under copious irrigation with 3% sodium hypochlorite. Patients were divided into two groups of 20 each: Group I (NaOCl) and Group II (CHX). The working length (WL) was determined in triplicates for each tooth using Root ZX apex locator and with S1 ProTaper rotary file in continuous motion in case of VDW Gold IAL in the presence of 3% sodium hypochlorite and 2% chlorhexidine. Therapeutic extraction was performed and WL was determined using size 15 K-Files under 20× magnification. RESULTS: The paired sample independent t test showed that there was no significant difference between the Root ZX and VDW apex locator in determining the minor foramen in NaOCl group (P = 0.234, 0.453 respectively) and CHX group (P = 0.085, 0.087) when compared with actual working length. CONCLUSION: Both the apex locators were equally effective in determining WL at 0.5 mm from the apex in presence of irrigating solutions, that is, NaOCl and chlorhexidine.

Accuracy of electronic apex locator in enlarged root canals with different root canal irrigants: an in vitro study.

AIM: To evaluate the accuracy of Root ZX-II (J Morita Corp) apex locator in enlarged root canals with different root canal irrigants. MATERIALS AND METHODS: 48 freshly extracted single rooted mandibular premolar teeth were used. The apical enlargement was done up to #45 K-file as the master apical file (MAF). The teeth were randomly divided into 4 groups and mounted in an experimental apparatus. The following irrigants were used during electronic canal measurements: group 1: saline; group 2: 3% NaOCl; group 3: 2% chlorhexidine; group 4: 17% EDTA. The canal measurement was done with Root ZX-II apex locator using #10 and #45 K-file. Data were analyzed by two way ANOVA and Bonferroni multiple comparison test. RESULTS: Statistical analysis showed that there was statistically significant difference between #10 and #45 K-file for group 1 (p < 0.01). CONCLUSION: Root ZX-II was accurate in the presence of 3% NaOCl and 17% EDTA when measured with smaller and larger files. However, it was accurate in the presence of saline and 2% CHX when larger files were used.

A rare presentation of bilateral maxillary dens invaginatus diagnosed using cone beam computed tomography.

Dens invaginatus is a developmental variation in the formation of a tooth that causes changes in the internal anatomy of the tooth. The presence of double dens invaginatus is extremely rare. Understanding the type, extension, and complex morphology of dens invaginatus is essential. Diagnosis of this condition using conventional radiographic techniques is not easy. Advanced imaging techniques, such as cone beam computed tomography (CBCT) are very helpful in diagnosis of these complex anatomic variations. In the present case report, we demonstrate the use of CBCT in the evaluation and treatment planning of bilateral maxillary dens invaginatus, of which one presented as a case of double dens.