Optical interrogation of neuronal circuitry in zebrafish using genetically encoded voltage indicators.

Optical measurement of membrane potentials enables fast, direct and simultaneous detection of membrane potentials from a population of neurons, providing a desirable approach for functional analysis of neuronal circuits. Here, we applied recently developed genetically encoded voltage indicators, ASAP1 (Accelerated Sensor of Action Potentials 1) and QuasAr2 (Quality superior to Arch 2), to zebrafish, an ideal model system for studying neurogenesis. To achieve this, we established transgenic lines which express the voltage sensors, and showed that ASAP1 is expressed in zebrafish neurons. To examine whether neuronal activity could be detected by ASAP1, we performed whole-cerebellum imaging, showing that depolarization was detected widely in the cerebellum and optic tectum upon electrical stimulation. Spontaneous activity in the spinal cord was also detected by ASAP1 imaging at single-cell resolution as well as at the neuronal population level. These responses mostly disappeared following treatment with tetrodotoxin, indicating that ASAP1 enabled optical measurement of neuronal activity in the zebrafish brain. Combining this method with other approaches, such as optogenetics and behavioural analysis may facilitate a deeper understanding of the functional organization of brain circuitry and its development.

Changes in the pharyngeal airway space and hyoid bone position after mandibular setback surgery for skeletal Class III jaw deformity in Japanese women.

INTRODUCTION: The purpose of the study was to examine the changes in stability of pharyngeal airway space (PAS) and hyoid bone position after 2 types of mandibular osteotomies in comparison with a control group. METHODS: The subjects included 46 Japanese women with skeletal Class III malocclusion. Twenty-five patients with mandibular prognathism underwent single-jaw surgery with bilateral sagittal split ramus osteotomy (SSRO), and 21 patients underwent bilateral intraoral vertical ramus osteotomy (IVRO). The control subjects included 30 volunteer women with normal occlusion. The treated subjects were assessed at the beginning of treatment, immediately after surgery, and after postsurgical treatment. RESULTS: The Class III subjects had a significantly wider PAS than did the control subjects. Significant decreases in the lower PAS were observed after orthognathic surgery. The hyoid bone showed upward and forward movement with upward movement of the lower border of the PAS during the postsurgical stage in the SSRO group. In contrast, the anterior border of the PAS and the hyoid bone showed considerable backward movement in the IVRO group. CONCLUSIONS: The posttreatment morphology of the PAS in both Class III groups approached that of the control group. The IVRO group showed a reduction in the airway dimensions, especially during the postsurgical period, which occurred during surgery in the SSRO group.

Feasibility of navigator setting on the left diaphragm for whole-heart coronary MRA: a study in healthy volunteers.

PURPOSE: We prospectively compared the quality of images obtained by navigator setting on the left and right diaphragm on whole-heart coronary magnetic resonance angiography (WHCMRA). METHODS: In 10 healthy volunteers, we performed free-breathing, 3-dimensional segmented true fast imaging with steady-state precession (trueFISP) WHCMRA by setting the navigator on the left and right diaphragm in random order. For the left diaphragm, we set the navigator outside the scope of the heart to avoid the influence on coronary arteries. We compared image acquisition time and visible length of coronary arteries using paired t-test and subjective image quality on a 4-point scale (1, poor; 4, excellent) using Wilcoxon signed-rank test. RESULTS: Mean overall subjective image quality was significantly better in the left diaphragm than the right (3.3+/-0.7 versus 2.9+/-0.9, P=0.02). Mean overall visible length of the coronary arteries was significantly better in the left diaphragm than the right (115.4+/-31.1 vs. 112.6+/-29.9 mm, P=0.02). Mean acquisition time between the left and right diaphragm was not significantly different (15.6+/-5.0 versus 16.0+/-5.7 min, P=0.79). CONCLUSION: In this small group of healthy volunteers, navigator setting for WHCMRA was superior on the left diaphragm than the right; however, feasibility of the technique requires additional consideration in a larger group of actual patients.

Frequency of common bile duct motion artifacts caused by inferior vena cava pulsation on magnetic resonance cholangiopancreatography.

PURPOSE: We assessed the frequency of common bile duct (CBD) motion artifacts caused by inferior vena cava (IVC) pulsation on magnetic resonance cholangiopancreatography (MRCP). METHODS: We retrospectively evaluated CBD motion artifacts in 4 MRCP sequences from each of 115 consecutive patients. RESULTS: We observed 37 (32.2%) ghost artifacts at the ventral and dorsal aspects of the CBD on transaxial, half-Fourier acquisition single-shot turbo spin-echo (HASTE-ax) images; no such artifacts were observed on transaxial T(2)-weighted turbo spin-echo images. In 10 patients, we observed 9 (7.8%) pseudo-defects of the CBD on 3-dimensional T(2)-weighted turbo spin-echo with navigator-triggered prospective acquisition correction technique MRCP and 6 (5.2%) pseudo-defects on single-shot rapid acquisition with relaxation enhancement MRCP. Pseudo-defects were significantly more frequent in patients with ghost artifacts than without (9 of 37 [24.3%] versus one of 78 [1.3%]; P<0.01, McNemar test). CONCLUSION: Although uncommon, pseudo-defects of the CBD caused by IVC pulsation are observed on MRCP. MRCP interpretation that includes comparison with HASTE-ax images can diminish the potential misinterpretation of such CBD motion artifact as bile duct tumor or biliary stone.