Subject(s)
Catheterization, Central Venous/methods , Jugular Veins , Arteries/injuries , Catheterization, Central Venous/adverse effects , Catheterization, Central Venous/instrumentation , Central Venous Pressure/physiology , Humans , Intubation/instrumentation , Regional Blood Flow/physiology , Reproducibility of Results , RespirationABSTRACT
An attempt was made to isolate Yersinia enterocolitica, by cold enrichment, from the stool of 1,212 patients seen at the Wesley Medical Center in Wichita, Kansas. Y. enterocolitica was isolated from 5 (0.4%) of the patients. Shigella was isolated from 36 (3.0%) patients while either Salmonella or Campylobacter were each isolated from 15 (1.2%) patients. Two of our five Yersinia isolates were from patients admitted to the hospital with diarrhea. One was from a patient who developed transient diarrhea while in the hospital, and the other two were from patients without diarrhea. In our opinion, the data do not justify the routine examination of stool specimens for Y. enterocolitica.
Subject(s)
Feces/microbiology , Yersinia/isolation & purification , Aged , Diarrhea/microbiology , Female , Gastroenteritis/etiology , Humans , Kansas , Male , Middle AgedABSTRACT
In 4 patients with lower extremity pain and either negative or equivocal radiograhic findings, the bone scan was definitely positive for stress fracture in all, accurately localized the lesion, and permitted proper therapy. The condition in all instances proved to be a microfracture at the origin or insertion of a muscle. The pain was always intensified by physical activity.
Subject(s)
Bone and Bones/diagnostic imaging , Pain/diagnostic imaging , Adult , Bone and Bones/injuries , Diagnosis, Differential , Female , Fractures, Bone/diagnostic imaging , Hip/diagnostic imaging , Hip Injuries , Humans , Leg/diagnostic imaging , Leg Injuries/therapy , Male , Pain Management , Physical Exertion , Radionuclide Imaging , Sports , Tibia/diagnostic imagingABSTRACT
The incidence of Campylobacter enteritis was assessed in an 800-bed medical center in the midwestern United States. Stool specimens from 203 patients were cultured on a modified Skirrow's selective media and incubated for 48 hours at 42 C in a microaerophilic atmosphere. Campylobacter fetus ssp. jejuni was isolated from 10 patients for an incidence rate of 4.9 percent. All patients were symptomatic. The results suggest that clinical laboratories must establish methods to culture and identify this organism on a routine basis.
Subject(s)
Campylobacter Infections/epidemiology , Enteritis/microbiology , Adolescent , Adult , Africa , Campylobacter fetus/isolation & purification , Canada , Child , Child, Preschool , Europe , Female , Humans , Infant , Male , Middle Aged , United StatesABSTRACT
A selected review is presented of the history of Group B streptococcal infections and the laboratory tests used to identify Group B streptococcus (Streptococcus agalactiae), an opportunistic pathogen which causes serious infections in newborn infants and compromised patients in other age groups. Its isolation may be increased by growth in a selective medium. The methods for Group B streptococci identification include the hydrolysis of sodium hippurate, the CAMP reaction, pigment production, and antibiotic disk susceptibility. Also, immunological tests, such as Lancefield's classical precipitin test, immunofluorescence staining, counterimmunoelectrophoresis, and coagglutination are available.
Subject(s)
Bacteriological Techniques , Streptococcus agalactiae/isolation & purification , Culture Media , Hemolytic Plaque Technique , Hippurates , Hydrolysis , Microbial Sensitivity Tests , Pigments, Biological/metabolism , Precipitin Tests , Streptococcal Infections/diagnosis , Streptococcal Infections/microbiology , Streptococcus agalactiae/metabolismABSTRACT
Commercial systems designed and marketed for evaluation of Enterobacteriaceae are used in many clinical microbiology laboratories. Evaluations of these systems have been reported from several large laboratories. Identifications with one or more of these systems were compared with those obtained by conventional methods with many strains of organisms. The Microbiology Resource Committee of the College of American Pathologists designed two surveys to evaluate the performances of commercial systems with identical strains of organisms submitted to a large number of participants. The surveys used were the Special Bacteriology Survey D-D and the Comprehensive Microbiology Survey B-D submitted during 1975--1976. Single strains of Klebsiella pneumoniae and Serratia marcescens in pure culture were submitted in the Comprehensive Survey and the same strains of these organisms plus one strain each of Pseudomonas stuartii and Citrobacter freundii in the Special Survey. The data obtained from participants permitted comparisons to be made between the API-20E, Enterotube, and r/b systems. These comparisons included individual biochemical test results shared by at least two systems as well as the ability of each to identify the unknown organisms to genus alone and to both genus and species. The results are presented and subjected to statistical analysis.
Subject(s)
Enterobacteriaceae/isolation & purification , Microbiological Techniques/standards , Bacteriological Techniques/standards , Enterobacteriaceae/classification , Evaluation Studies as Topic , Humans , Laboratories/standards , Pathology , Reference Standards , Societies, Medical , United States , Urine/microbiologyABSTRACT
A case of subacute bacterial endocarditis due to Streptococcus mutans is presented. The case was successfully treated by intravenous and oral penicillin. Streptococcus mutans is a member of the viridans streptococcal group with properties similar to enterococcal streptococci. Since the enterococci are resistant to penicillin, and isolates of Streptococcus mutans are usually sensitive to penicillin, it is important that medical technologists and microbiologists accurately identify these organisms. The combination of the following characteristics: 1) lack of Group D antigen, 2) acid formation in mannitol broth, 3) failure to hydrolyze hippurate, and 4) formation in five per cent sucrose broth of gelatinous adherent deposits on walls and bottom of tube, distinguish Streptococcus mutans from enterococcal streptococci.
