Effectiveness of pig sludge as organic amendment of different textural class mine tailings with different periods of amendment-contact time.

The present study assesses the effect of tailing texture (loamy sand (LT) and sandy loam (ST)), dose of pig sludge (0, 50, 100 and 200 t ha-1) and amendment-contact time (14, 28 and 42 days) on physicochemical quality of amended substrate using Lolium perenne var Nui as a bioindicator. The main properties of LT differed of ST in levels of total organic carbon (0.19 and 0.58%), in pH (4.6 and 8.5), total Cu (202 and 1647 mg kg-1) and Zn content (31 and 137 mg kg-1). Soil pore water of experimental substrates was characterized for pH, electrical conductivity (EC) and Cu2+ ion activity (pCu2+) while ammonium nitrogen (NH4+-N), infiltration rate and general physicochemical characteristics were measured in substrates. Shoot biomass (SB), root biomass (RB) and the RB:SB ratio was calculated for L. perenne. The results showed there to be a significant interaction (p < 0.05) between tailing texture, sludge dose and amendment-contact time for pCu2+, infiltration rate, SB, RB, and RB: SB ratio, but not for pH, EC, or NH4+-N. However, sludge dose and amendment-contact time significantly affected all variables. By increasing dosages of pig sludge, pore water pH increased, and this was associated with decreases in pCu2+ and the infiltration rate. High doses of pig sludge (100 and 200 t ha-1) impaired growth of L. perenne irrespective of tailing texture and amendment-contact time, likely because of the rise of EC (up to 14 mS cm-1). For both tailing textures, the highest biomass was obtained after incorporation of 50 t ha-1 of pig sludge, with increasing values as amendment-contact time rose. In conclusion, effective management of pig sludge for tailing reclamation should guarantee doses <50 t ha-1 and amendment-contact time >28 days, irrespective of tailing texture.

Phytoremediation of Cu and Zn by vetiver grass in mine soils amended with humic acids.

Phytoremediation of contaminated mine soils requires the use of fast-growing, deep-rooted, high-biomass, and metal-tolerant plants with the application of soil amendments that promote metal uptake by plants. A pot experiment was performed to evaluate the combined use of vetiver grass (Chrysopogon zizanioides) and humic acid for phytoremediation of Cu and Zn in mine soils. Vetiver plants were grown in soil samples collected from two mine sites of Spain mixed with a commercial humic acid derived from leonardite at doses of 0, 2, 10, and 20 g kg(-1). Plant metal concentrations and biomass were measured and metal bioavailability in soils was determined by a low molecular weight organic acid extraction. Results showed that humic acid addition decreased organic acid-extractable metals in soil. Although this extraction method is used to estimate bioavailability of metals, it was not a good estimator under these conditions due to competition with the strong chelators in the added humic acid. High doses of humic acid also promoted root growth and increased Cu concentrations in plants due to formation of soluble metal-organic complexes, which enhanced removal of this metal from soil and its accumulation in roots. Although humic acid was not able to improve Zn uptake, it managed to reduce translocation of Zn and Cu to aerial parts of plants. Vetiver resulted unsuitable for phytoextraction, but our study showed that the combined use of this species with humic acid at 10-20 g kg(-1) could be an effective strategy for phytostabilization of mine soils.

Soluble organic carbon and pH of organic amendments affect metal mobility and chemical speciation in mine soils.

We evaluated the effects of pH and soluble organic carbon affected by organic amendments on metal mobility to find out the optimal conditions for their application in the stabilization of metals in mine soils. Soil samples (pH 5.5-6.2) were mixed with 0, 30 and 60 th a(-1) of sheep-horse manure (pH 9.4) and pine bark compost (pH 5.7). A single-step extraction procedure was performed using 0.005 M CaCl2 adjusted to pH 4.0-7.0 and metal speciation in soil solution was simulated using NICA-Donnan model. Sheep-horse manure reduced exchangeable metal concentrations (up to 71% Cu, 75% Zn) due to its high pH and degree of maturity, whereas pine bark increased them (32% Cu, 33% Zn). However, at increasing dose and hence pH, sheep-horse manure increased soluble Cu because of higher soluble organic carbon, whereas soluble Cu and organic carbon increased at increasing dose and correspondingly decreasing pH in pine bark and non-amended treatments. Near the native pH of these soils (at pH 5.8-6.3), with small doses of amendments, there was minimum soluble Cu and organic carbon. Pine bark also increased Zn solubility, whereas sheep-horse manure reduced it as soluble Zn always decreased with increasing pH. Sheep-horse manure also reduced the proportion of free metals in soil solution (from 41% to 4% Cu, from 97% to 94% Zn), which are considered to be more bioavailable than organic species. Sheep-horse manure amendment could be efficiently used for the stabilization of metals with low risk of leaching to groundwater at low doses and at relatively low pH, such as the native pH of mine soils.

Chemical speciation and mobilization of copper and zinc in naturally contaminated mine soils with citric and tartaric acids.

A one-step extraction procedure and a leaching column experiment were performed to assess the effects of citric and tartaric acids on Cu and Zn mobilization in naturally contaminated mine soils to facilitate assisted phytoextraction. A speciation modeling of the soil solution and the metal fractionation of soils were performed to elucidate the chemical processes that affected metal desorption by organic acids. Different extracting solutions were prepared, all of which contained 0.01 M KNO(3) and different concentrations of organic acids: control without organic acids, 0.5 mM citric, 0.5 mM tartaric, 10 mM citric, 10 mM tartaric, and 5 mM citric +5 mM tartaric. The results of the extraction procedure showed that higher concentrations of organic acids increased metal desorption, and citric acid was more effective at facilitating metal desorption than tartaric acid. Metal desorption was mainly influenced by the decreasing pH and the dissolution of Fe and Mn oxides, not by the formation of soluble metal-organic complexes as was predicted by the speciation modeling. The results of the column study reported that low concentrations of organic acids did not significantly increase metal mobilization and that higher doses were also not able to mobilize Zn. However, 5-10 mM citric acid significantly promoted Cu mobilization (from 1 mg kg(-1) in the control to 42 mg kg(-1) with 10 mM citric acid) and reduced the exchangeable (from 21 to 3 mg kg(-1)) and the Fe and Mn oxides (from 443 to 277 mg kg(-1)) fractions. Citric acid could efficiently facilitate assisted phytoextraction techniques.

Dynamics of pruning waste and spent horse litter co-composting as determined by chemical parameters.

Co-composting of pruning waste and horse manure was monitored by different parameters. A windrow composting pile, having the dimensions 2.5m (height) x 30m (length) was established. The maturation of pruning waste and horse manure compost was accompanied by a decline in NH(4)(+)-N concentration, water soluble C and an increase in NO(3)(-)-N content. Organic matter (OM) content during composting followed a first-order kinetic equation. This result was in agreement with the microbiological activity measured by the CO(2) respiration during the process. The correlation at a high level of probability found between the OM loss and CO(2) evolution showed that both parameters could be used to indicate the degree of OM degradation that is the maturity and stability phases of the compost studied. Humification parameters data from the organic matter fractionation did not show a clear tendency during the composting time, suggesting that these parameters are not suitable for evaluating the dynamics of the process.

Chemical and physical properties of pruning waste compost and their seasonal variability.

Selected chemical and physical properties of 12 different pruning waste compost (PWC) samples were evaluated to assess their suitability as substrates for ornamental plants. Samples were taken periodically from the same composting facility over 18 months in order to determine if there was any seasonal variability. In addition to the PWC samples, a Canadian Sphagnum peat and a commercial growing medium (CGM) were used as standard materials. With respect to PWC properties, pH values were above 8, significantly higher than the pH of peat and the commercial substrate. All samples showed adequate levels of organic matter and correspondingly high cation-exchange capacity (CEC) values. The C/N ratio varied between 22 and 48, significantly higher than the optimal values of 15-20. Although composts were sampled from piles established in different seasons, no significant differences were found in their chemical properties. However, water retention characteristics were affected by seasonal changes in components entering the facility. Comparing the properties of PWC to those of peat and commercial growing medium, this material appears to be an acceptable component of a substrate for container-grown ornamental plants.

Use of pruning waste compost as a component in soilless growing media.

The objective of this work was to study the use of pruning wastes compost (PWC) as a growing media component for ornamental plants. The main physical, chemical and biological characteristics of PWC were analysed in order to evaluate its suitability for use in soil-less cultivation. Six growth substrates were prepared by mixing PWC with peat (P), ground leaves (GL), sand (S) and spent mushroom compost (SMC) in different proportions. Two different pot experiments were carried out to test its characteristics of production using perennial ryegrass (Lolium perenne L.) and cypress (Cupressus sempervirens L.) as indicators and the different media as treatments. The growth experiments showed that PWC required mixing with a nutrient-richer material to produce higher results. Therefore, substrates containing SMC (PWC+P+SMC and PWC+SMC) seems to be the most adequate growing media. After the statistical analysis, we concluded that the PWC could be used as a growing media component.