ABSTRACT
BACKGROUND/AIMS: The present study was undertaken to analyze the beta2-microglobulin (BMG)-removing efficiency of high-performance membranes (HPMs) in relation to changes in serum BMG levels after dialysis. METHODS: In 10 stable dialysis patients, six different HPMs were used in a crossover method. Serum BMG levels were measured immediately before dialysis, immediately after, 4 h after, and immediately before the next dialysis. On the basis of these measurements, the BMG removal rate was calculated. RESULTS: The BMG removal rate as calculated on the basis of the BMG level immediately after dialysis was 11.29% higher than the rate calculated on the basis of the BMG level 4 h after dialysis. CONCLUSION: The BMG removal rate can be overestimated if it is calculated on the basis of the BMG level immediately after dialysis.
Subject(s)
Kidneys, Artificial , Membranes, Artificial , Renal Dialysis/instrumentation , beta 2-Microglobulin/analysis , Cross-Over Studies , Humans , Kidney Failure, Chronic/blood , Kidney Failure, Chronic/therapy , Time Factors , beta 2-Microglobulin/metabolismABSTRACT
The unicellular cyanobacterium Microcystis aeruginosa K-81 has two types of restriction barrier, an extracellular nuclease and sequence-specific endonucleases. The nuclease was detected in the culture supernatant and it was easily released from the cells by washing with water or buffer containing Triton X-100. This nuclease was identified as a polypeptide of about 28 kDa that digested covalently closed circular and linear double-stranded DNAs, including chromosomal DNA from M. aeruginosa K-81. Among another 13 Microcystis strains examined, 3 produced an extracellular nuclease. Furthermore, M. aeruginosa K-81 contained two sequence-specific endonucleases, MaeK81I and MaeK81II, which were isoschizomers of SplI and Sau96I, respectively.
