ABSTRACT
Here, we report a non-invasive strategy for isolating cancer cells by autonomously propelled carbon nanotube (CNT) microrockets. H2O2-driven oxygen (O2) bubble-propelled microrockets were synthesized using CNT and Fe3O4 nanoparticles in the inner surface and covalently conjugating transferrin on the outer surface. Results show that self-propellant microrockets can specifically capture cancer cells.
Subject(s)
Nanotubes, Carbon/chemistry , Neoplastic Cells, Circulating/chemistry , Ferrosoferric Oxide/chemistry , HCT116 Cells , Humans , Hydrogen Peroxide/chemistry , Ligands , Magnetite Nanoparticles/chemistry , Microscopy, Electron, Transmission , Oxygen/chemistry , Surface Properties , Time-Lapse ImagingABSTRACT
A multicomponent magneto-dendritic nanosystem (MDNS) is designed for rapid tumor cell targeting, isolation, and high-resolution imaging by a facile bioconjugation approach. The highly efficient and rapid-acting MDNS provides a convenient platform for simultaneous isolation and high-resolution imaging of tumor cells, potentially leading towards an early diagnosis of cancer.
Subject(s)
Cell Separation/methods , Immunomagnetic Separation/methods , Molecular Diagnostic Techniques/methods , Nanoparticles , Neoplastic Cells, Circulating/metabolism , Neoplastic Cells, Circulating/pathology , Transferrin/pharmacokinetics , Hep G2 Cells , Humans , Immunomagnetic Separation/instrumentation , Molecular Diagnostic Techniques/instrumentation , Nanoparticles/chemistryABSTRACT
We describe a novel multicomponent graphene nanostructured system that is biocompatible, and has strong NIR optical absorbance and superparamagnetic properties. The fabrication of the multicomponent nanostructure system involves the covalent attachment of 3 components; Fe(3)O(4)(Fe) nanoparticles, PAMAM-G4-NH(2) (G4) dendrimer and Cy5 (Cy) on a graphene oxide (GO) surface to synthesize a biologically relevant multifunctional system. The resultant GO-G4-Fe-Cy nanosystem exhibits high dispersion in an aqueous medium, and is magnetically responsive and fluorescent. In vitro experiments provide a clear indication of successful uptake of the GO-G4-Fe-Cy nanosystem by MCF-7 breast cancer cells, and it is seen to behave as a bright and stable fluorescent marker. The study also reveals varied cellular distribution kinetics profile for the GO nanostructured system compared to free Cy. Furthermore, the newly developed GO nanostructured system is observed to be non-toxic to MDA-MB-231 cell growth, in striking contrast to free G4 dendrimer and GO-G4 conjugate. The GO-G4-Fe-Cy nanostructured system characterized by multifunctionality suggests the merits of graphene for cellular bioimaging and the delivery of bioactives.
