An extended colloid filtration theory for modeling Escherichia coli transport in 3-D fracture networks.

Microbial transport in fractured carbonate rock using enhanced solutions is a significant and neglected research topic in the literature. We propose an extended colloid filtration theory (CFT) combined with a particle-tracking following streamlines (PTFS) model for the rapid prediction of breakthrough curves (BTCs) and plumes of pathogens in three-dimensional (3-D) discrete fracture networks (DFNs). We adapted CFT in porous media to pathogen transport in fractures containing Terra Rossa (soil) deposits. As an example of the model capability, a simulation was used to predict the 3-D motion field and Escherichia coli count in groundwater originating from the Forcatella managed aquifer recharge (MAR) Facility (Brindisi, Italy) using a DFN composed of 3,900 fractures. In arid regions, MAR facilities are significant for sustaining basic human needs, such as freshwater supply for drinking and crop production. The Markov chain Monte Carlo (MCMC) technique was applied to E. coli counts in the collected water samples to increase data representativeness. The pathogen transport coefficients were further supported by batch filtration tests carried out in the CNR/IRSA Laboratory (Bari, Italy). The mean E. coli attachment rate coefficient of 0.15 × 10-8 m2 d-1 (sticking efficiency = 1.1 × 10-8 m) resulted in a 2.1 log10 removal in 600 m of reclaimed water filtration. The simulation output visualized the E. coli 3-D pathways in groundwater and the positions of contaminated groundwater spring outflows on Forcatella Beach. The simulation results agreed with the mean MCMC output of E. coli concentrations in bathing water under unperturbed geochemical and environmental flow and transport conditions. However, results indicate that concentrations of pathogenic strains, parasites, and enteric viruses may enter the marine environment of MAR sites during flood periods.

Fate and transport of faecal contamination microbial indicators, pathogenic protozoa and Campylobacter in the artificially recharged fractured aquifer of Salento, Italy.

The study investigates the fate and transport of microorganisms introduced by artificial groundwater recharge at the Nardò fractured aquifer in Salento, Italy. Microbial indicators of faecal contamination, parasitic protozoa (Giardia and Cryptosporidium) and pathogenic bacteria (Campylobacter spp.), were monitored into injected water and groundwater to test the efficiency of the "natural disinfection" into the fractured aquifer. A remarkable decrease of microbial indicators and pathogens was observed suggesting that pathogens removal or inactivation may be possible during water flow in fractured aquifer. The recently described PNA probe CJE195 (Lehtola et al. 2005) was utilised for the rapid and specific detection of Campylobacter spp. by fluorescence in situ hybridization (FISH) after enrichment. FISH results were consistent with those of traditional cultural method (ISO 17995) applied in parallel: time required for Campylobacter identification was reduced of 4 days.

Assessment of ground water quality in a fractured aquifer under continue wastewater injection.

Experimental studies have been carried out in a fractured coastal aquifer of the Salento Region (Nardò (Le), Italy), subject since 1991 to injection of 12,000 m3/d of treated municipal wastewater in a natural sink. The analytical parameters of ground water sampled in monitoring wells, have been compared before and after the injection started. The mound of water table (1.5 m), the reduction of seawater extent of 2 km and the spreading of pollutants injected were evaluated by means of mathematical model results. After ten years operation, the volume of the available resource for agricultural and drinking use has been increased, without notable decrease of the preexistent ground water quality. Moreover for preserving such resource from pollution, the mathematical model allowed the standards of wastewater quality for recharge to be identified. Around the sink, a restricted area was also defined with prohibition of withdrawals, to avoid infection and other risks on human health.