Acute free fatty acid elevation eliminates endurance training effect on insulin sensitivity.

CONTEXT: Both training and normal body mass index are associated with high insulin sensitivity, but the mechanism may be different. OBJECTIVE: The aim of the study was to examine whether lean trained humans may be protected from acute free fatty acid (FFA)-induced insulin resistance compared with lean sedentary humans. DESIGN AND SETTING: We conducted an interventional trial using either a 6-h lipid (20% Intralipid at 90 ml/h) or glycerol (2.25 g/100 ml at 90 ml/h) infusion along with a concurrent hyperinsulinemic-euglycemic clamp and serial muscle biopsies (0, 120, 360 min) at a clinical research unit at the University of Minnesota. PATIENTS OR PARTICIPANTS: The study included lean endurance-trained (n = 14) and sedentary (n = 14) individuals matched for age, gender, and body mass index. MAIN OUTCOME MEASURES: We measured the decline in glucose infusion rate (GIR) during the hyperinsulinemic-euglycemic clamp. RESULTS: The trained group had higher baseline mitochondrial DNA copy number, mRNA of cytochrome C oxidase subunit 3, and insulin sensitivity (as measured by GIR) compared with the sedentary group. When FFA was acutely elevated to the upper physiological range (0.6-0.7 mEq/liter) by lipid infusion, the GIR in both activity groups declined similarly compared with their respective glycerol controls, although insulin signaling, as measured by Ser 473 pAKT/AKT, remained comparable. Specific to the trained group, the stimulatory effect of hyperinsulinemia on mitochondrial mRNA levels during the glycerol infusion was absent during the lipid infusion. CONCLUSIONS: Elevated FFA had similar effects in reducing insulin sensitivity in trained and sedentary humans. In trained participants, this decline was associated with alterations in the skeletal muscle mitochondrial mRNA response to hyperinsulinemia.

Dry matter intake and energy balance in the transition period.

It is easy, based on theoretic considerations, to make the argument that maximizing DMI is important to minimize postpartum complications and losses in milk production that may be associated with them. However, research over the past several years provides ample evidence that successful "passage" through the periparturient period is more complicated than simply maximizing feed intake. Anecdotal evidence from veterinarians and nutrition consultants also confirm that feeding low-NDF diets to achieve high prepartum feed intakes during the prefresh transition period does not necessarily solve fresh-cow problems. Perhaps more important than maximizing feed intake is to minimize the likelihood of cows experiencing large drops in feed intake immediately before parturition. Retrospective analysis of existing data sets indicates that this hypothesis has merit; research must be conducted to vigorously test it. Until then, it seems reasonable to try to achieve high DMI, if it can be sustained through parturition. If it cannot, perhaps a more conservative approach is to limit voluntary intake by increasing dietary fiber, because data suggests that cows fed in such a manner experience less dramatic decreases in feed intake as parturition approaches. We examined the importance of parity, body condition score, and various diet components that may influence DMI during the final 3 weeks before parturition, but they only explained 18% of the variation in intake among cows. Clearly, there are many other factors that affect intake that need to be identified. Aspects of farm management that may influence animal stress need to be investigated, particularly during the prefresh transition period when cows are inherently prone to reductions in feed intake.