[Therapeutic bacterial vaccine Immunovac in complex treatment of patients with chronic pyoderma].

AIM: Assessment of therapeutic effect and immunologic parameters during use of Immunovac vaccine for complex treatment of chronic forms of pyoderma. MATERIALS AND METHODS: Ninety-five patients with different clinical forms of chronic pyoderma (furunculosis, hydradenitis, chronic ulcerative and ulcerative-vegetans pyoderma, folliculitis, impetigo etc.) were studied. Fifty-nine patients received immunotherapy with Immunovac vaccine together with basic therapy and 36 patients comprised control group treated only with basic therapy. Studied immunologic parameters were as follows: assessment of functional activity of lymphocytes, determination of lymphocyte subpopulations by flow cytometry, total immunoglobulins classes A, G, M by radial immunoduffusion, affinity of antibodies by enzyme immunoassay, levels of IFNalpha and IFNgamma. RESULTS: Use of Immunovac vaccine in complex treatment of patients with chronic forms of pyoderma enhanced clinical effect of basic therapy, which expressed in decrease of severity and frequency of disease relapses irrespective to clinical form and severity of pyoderma. Therapeutic effect during use of Immunovac vaccine amounted 84.7%, whereas in control group it was 41.6% after 12 months of follow-up. Increase of functional activity of neutrophils, subpopulation of lymphocytes with markers CD4+, CD8+, CD72+, affinity of antibodies as well as induced production of IFNalpha and IFNgamma was revealed. Correction of immunologic parameters correlated with positive results of patients treatment. CONCLUSION: Inclusion of bacterial polycomponent vaccine Immunovac in complex treatment of patients with chronic pyoderma promotes enhancement of therapeutic effect of basic therapy and correction of immunologic parameters.

[The efficacy and reactogenicity of a cell-free staphylococcal vaccine in the immunotherapy of patients with chronic pyodermas]. / Effektivnost' i reaktogennost' beskletochnoi stafilokokkovoi vaktsiny pri immunoterapii bol'nykh khronicheskimi piodermiiami.

The clinical effectiveness, reactogenicity and immunological activity of cell free staphylococcal vaccine in the immunotherapy of chronic pyoderma patients under the conditions of controlled trials were studied. Immunotherapy was carried out in combination with traditional methods of treatment. A group of 39 patient received cell free staphylococcal vaccine subcutaneously; the second control group (30 patients) received the commercial preparation of staphylococcal antiphagin and the third group (31 patients) was treated by traditional methods without vaccines. The study revealed that cell free staphylococcal vaccine had low reactogenicity and was well tolerated by patients; in contrast to the methods of treatment in the control groups, the vaccine stimulated an increased levels of specific antibodies and IgA in the blood of the patients. Immunotherapy with cell free staphylococcal vaccine led to clinical convalescence and remission in 1-3 years (the term of observation) in 71.8% of cases. In the two control groups such effect did not exceed 23%.

[The effect of immunotherapy with cell-free vaccines made from the antigens of opportunistic microorganisms on the dynamics of DNA antibody formation]. / Izuchenie vliianiia immunoterapii beskletochnymi vaktsinami iz antigenov uslovno-patogennykh mikroorganizmov na dinamiku obrazovaniia antitel k DNK.

The sera of patients subjected to immunotherapy with staphylococcal vaccine and with multicomponent vaccine (i.e. the mixture of the antigenic preparations of Staphylococcus aureus, Klebsiella pneumoniae, Proteus vulgaris, Escherichia coli) were studied by the method of the enzyme immunoassay on the basis of cattle spleen DNA. Immunotherapy with staphylococcal vaccine was given to patients with dermal diseases, chronic obstructive bronchitis and pulmonary abscess. Multicomponent vaccine was introduced to patients with the infectious allergic form of bronchial asthma, moderate or severe. Immunotherapy with both preparations under study was shown to produce no accumulation of antibodies to native and denatured DNA.

[Chronic pyoderma caused by beta-lactamase-producing strains of Staphylococcus]. / Khronicheskaia piodermiia, obuslovlennaia beta-laktamazoprodutsiruiushchimi shtammami stafilokokka.

Features of the agent structure have been revealed on a subcellular level in vitro and in vivo in the patients with chronic pyodermas induced by Staphylococcus beta-lactamase-producing strains, and the type of these strains interaction with phagocytes before and in the course of combined therapy detected. Regularities in morphologic manifestations of pyoderma development were singled out, explained by specific features of cellular exudate composition and interactions between bacteria and eukaryotes.

[Iridoscopy in the diagnosis of internal pathology in dermatoses]. / Iridoskopiia v diagnostike vnutrennei patologii pri nekotorykh dermatozakh.

Iridoscopy was carried out in 40 patients with rosacea and seborrheic dermatitis in order to detect visceral abnormalities. Various gastrointestinal diseases were revealed in 32, vascular and neuropsychic disorders in 16, and renal or pancreatic shifts in 5 patients.

[Structuro-functional properties of staphylococci in patients with pyoderma]. / Strukturno-funktsional'nye osobennosti stafilokokkov u bol'nykh piodermiami.

Electron-microscopic and ultra-cytochemical studies have shown the structural and functional features of pyoderma agents--staphylococci isolated from patients with acute and chronic pyoderma. The studies have revealed magnesium-activated ATPase and adenylate cyclase in these bacteria; the authors have tried to trace a correlation between these enzymes' activities and location and the disease pattern. This may help assess the bacterial population status and choose the drugs for purposeful pathogenetic therapy.

[Experience in using a tomicide paste in the combined treatment of patients with pyoderma]. / Opyt primeneniia tomitsidovoi pasty v kompleksnoi terapii bol'nykh piodermiiami.

Tomicide paste is a highly effective agent in combined therapy of patients with primary and secondary pyoderma. Tomicide is recommended for practical dermatology in the liquid form for sanitization++ of infection foci in nasopharyngeal organs and in paste with a 10% concentration of the agent. This paste is also advised for cases with secondary pyoderma complicating the course of neurodermatitis and chronic eczema not liable to weeping.

[Ultrastructural characteristics of a focus of recurrent infection in genital herpes patients (clinical and electron microscopy studies)]. / Ul'trastrukturnaia kharakteristika ochaga retsidiviruiushchei infektsii u bol'nykh genital'nym gerpesom (klinicheskoe i élektronno-mikroskopicheskoe issledovanie).

In patients with recurrent genital herpes in the active stage of herpetic infection, herpes simplex virus (HSV) is located both extra- and intracellularly in keratinocytes of the pricky and glandular layers of the epidermis as well as in macrophages and neutrophilic leukocytes. The observed incompleted phagocytosis of HSV by macrophages may be one of the causes of insufficient local resistance to the virus.