Aspects of "antigen-antibody" interaction of chicken infectious bronchitis virus determined by surface plasmon resonance.

Authors performed investigation on "antigen-antibody" interaction of chicken infectious bronchitis coronavirus (IBV) by a method based on the surface plasmon resonance (SPR). Presence of space-size effect related to a difference between antigen and antibody particle sizes has been theoretically grounded and experimentally proven. Herewith, the difference between responses of the SPR-sensor to specific and non-specific interactions is considerably less (up to 6.3 times) than the expected one (8 - 11 times). An impact of functionalization of sensor's sensitive element surface, as well as acidity of buffer solution on the activity of antigen-antibody interaction was studied here. The difference between sensor's responses to specific and non-specific interactions increased two-fold from 200 to 432ang sec due to this treatment. When changing the acidity of analyzed solution from pH7.3 to pH6.8, the corresponding difference between sensor's responses increased by 6.3 times from 194 up to 1235ang.sec. Thus, an impact of space-size effect on interaction between IBV antigen and specific antibody can be considerably (almost in 3 times) decreased by reducing the acidity of used buffer solution. The results of our investigation can be successfully applied to develop new methods for detection of pathogens and specific antibodies using SPR.

[The use of a method for the quantitative determination of HIV-1 RNA for assessing the severity and prognosis of the development of the disease]. / Ispol'zovanie metoda kolichestvennogo opredeleniia RNK VICh-1 dlia otsenki tiazhesti i prognoza razvitiia zabolevaniia.

The informatory role of a new marker of HIV infection, characterizing the content of HIV-1 RNA in the biological fluids of the patient's body, is evaluated. The quantitative determination of HIV-1 RNA, carried out in a single assay, was made in the blood of 25 HIV-infected patients. These studies confirmed that the determination of the level of RNA in the plasma (viral load) was a reliable criterion indicating the severity and progress of the disease. The viral load of more than 100,000 copies/ml was a sign prognosticating the future pronounced progress of the disease in spite of moderate clinical manifestations and relatively high values of CD4 cells in the patient's blood at the moment of testing.

[Endogenous intoxication syndrome in critical states in infants. New diagnostic and prognostic possibilities]. / Sindrom éndogennoi intoksikatsii pri kriticheskikh sostoianiiakh u detei rannego vozrasta. Novye diagnosticheskie i prognosticheskie vozmozhnosti.

New methods for the diagnosis of the endogenous intoxication syndrome (EIS) in critical states have been developed and tried in 585 infants. A complex of biochemical, hemorheological, biophysical, and optic polarization parameters characterizing the intensity of catabolic reactions and united under the term "endotoxicogram" is offered. Objective assessment of EIS is expressed by an integral index of intoxication which permits a universal assessment of the severity of patient's status, disease course, and efficacy of therapy and helps predict the disease outcome.

[Changes of the liver and bile ducts in patients with acute intestinal diseases]. / Izmeneniia pecheni i zhelchnykh putei u bol'nykh s ostrymi kishechnymi zabolevaniiami.

Patients with acute intestinal infections, in particular, salmonellosis showed in 1/3 during the acute period an insignificant increase of bilirubin and alaninaminotransferase. During early reconvalescence the majority of patients revealed functional changes of the biliary tract, mainly, in the form of hypotensive-hypokinetic type of dyskinesia.

[An evaluation of the vibration hazard of the hand-operated machines used in automobile manufacture]. / Otsenka vibroopasnosti ruchnykh mashin, ispol'zuemykh v avtomobilestroenii.

After the examination and hygienic assessment of 53 types of hand-held Soviet and foreign machines, 30 types of them were selected because according to their vibration and ergonomic characteristics they could be recommended for wide application in automotive industry. Some causes for deterioration of vibration characteristics of hand-held machines while they were in use were analyzed. Among the basic causes there were untimely running, preventive repair and vibration parameters control of the machines and also their mishandling. Introduction of the proposed pattern of machine movement and maintenance during their use could further the improvement of the technical state of hand-held machines and decrease of local vibration levels affecting workers.