[The influence of enzymatic removal of chlorpyrifos from feed grain-mixture on the biochemical parameters of rat blood]. / Vliianie fermentativnoi obrabotki kormovoi zernosmesi ot khlorpirifosa na biokhimicheskie pokazateli krovi krys.

Organophosphorus pesticides (OP) are used to protect crops from pests. Treatment of plants and animals with pesticides can be done during their growth or creation of conditions necessary for the long-shelf life of the agricultural products. Currently, there are many remedies for prevention and removal of intoxication consequences developed under the action of OP in living organisms. The development of biologics for the degradation of OP and biotechnologies for their application in agriculture is relevant. New biologics based on the stabilized forms of such enzyme as hexahistidine-tagged organophosphorus hydrolase (His6-OPH) in the form of nano-sized particles were tried for OP detoxification. These biologics (enzyme-polyelectrolyte complexes, EPC) were obtained in accordance to previously developed procedure by mixing solutions of His6-OPH and polyanion under certain conditions. The main purpose of this work was to evaluate the usage efficiency of EPC based on His6-OPH and polyglutamic acid for OP detoxification by analyzing biochemical blood parameters of rats consumed the grain-mixture containing chlorpyrifos. The experiment was conducted using female Sprague Dawley albino rats. Treatment of feeding grain-mixture initially containing chlopyrifos (48 mg/kg of the mixture) with EPC based on His6-OPH (1000 U/kg of the mixture) for 24 h was the most effective. The results showed that rats from the group consuming food after enzymatic removal of chlorpyrifos, had comparable acetyl cholinesterase activity in blood of rats consuming pure food (without any OP intoxication).

Production of biofuels from pretreated microalgae biomass by anaerobic fermentation with immobilized Clostridium acetobutylicum cells.

The purpose of this work was to study the possible use of pretreated biomass of various microalgae and cyanobacteria as substrates for acetone-butanol-ethanol (ABE) fermentation by Clostridium acetobutylicum cells immobilized into poly(vinyl alcohol) cryogel. To this end, the biochemical composition of photosynthetic microorganisms cultivated under various conditions was studied. The most efficient technique for pretreating microalgal biomass for its subsequent conversion into biofuels appeared to be thermal decomposition at 108 °C. For the first time the maximum productivity of the ABE fermentation in terms of hydrogen (8.5 mmol/L medium/day) was obtained using pretreated biomass of Nannochloropsis sp. Maximum yields of butanol and ethanol were observed with Arthrospira platensis biomass used as the substrate. Immobilized Clostridium cells were demonstrated to be suitable for multiple reuses (for a minimum of five cycles) in ABE fermentation for producing biofuels from pretreated microalgal biomass.