Investigation of the relationship between miR-33a, miR-122, erythrocyte membrane fatty acids profile, and serum lipids with components of metabolic syndrome in type 2 diabetic patients.

Background and purpose: MicroRNAs (miRNAs) are small non-coding RNA molecules acting as critical regulators of post-transcriptional gene expression. MiR-33a and miR-122 have a crucial role in cholesterol and lipid metabolism. Therefore, their dysregulation may contribute to metabolic abnormality and their inhibition may be a useful therapeutic strategy. The objective of the present study was to investigate the relationship between miR-33a, miR-122, erythrocyte membrane fatty acids profile, and serum lipids with components of metabolic syndrome in an Iranian population suffering from type 2 diabetes mellitus (T2DM). Experimental approach: Expression of miR-33a and miR-122 was measured by real-time polymerase chain reaction and erythrocyte membrane fatty acid profiles were analyzed by gas chromatography-mass spectrometry. Findings/Results: T2DM patients with and without metabolic syndrome had significantly higher miR-33a and miR-122 levels compared to controls. MiRNAs were significantly correlated with saturated fatty acid (SFAs), total SFAs/total polyunsaturated fatty acids (PUFAs) ratio, fasting plasma glucose, triacylglycerols, insulin and homeostatic model assessment of insulin resistance. In addition, there was a significant negative correlation between miR-33a and miR-122 levels and PUFAs, total PUFAs/total SFAs ratio and omega 6 fatty acids. Conclusion and implications: Considering the roles of miR-33a and miR-122 in cholesterol and lipids metabolism, it may be concluded that the measurement of their expression may be useful as a potential additional biomarker for cardiometabolic derangement in T2DM patients. In addition, these findings may suggest that the inhibition of these miRNAs by anti-miRNA therapies may be explored as a potential therapeutic strategy.

Performance of new biodegradable chelants in enhancing phytoextraction of heavy metals from a contaminated calcareous soil.

Chelant-assisted phytoextraction has widely been exploited as a feasible option for removing heavy metals from the contaminated soils. Some synthetic chelants have shown promising performances for this option, but they have also revealed several negative environmental consequences. This study has sought to investigate the feasibility of two biodegradable eco-friendly chelants, namely methylglycinediacetic acid (MGDA) and N,N-Bis(carboxymethyl)-L-glutamic acid (GLDA), as compared to the resistant ethylenediaminetetraacetic acid (EDTA), in enhancing phytoextraction of Zn and Pb from a contaminated calcareous soil. For this purpose, a greenhouse experiment was carried out comparing the growth and metal absorption of maize (Zea mays L.) grown on soils treated with EDTA, MGDA, and GLDA chelants at 2, 4 and 8 mmol kg- 1 levels. Results showed that the heavy metal uptakes by the plant shoots generally increased with increasing the chelant application level. Pb uptake by maize shoots increased from 10.6 mg plant- 1 in control to 416, 398, and 416 mg plant- 1 in the soils treated with 8 mmol kg- 1 MGDA, GLDA, and EDTA, respectively. The corresponding increases in Zn uptake were from 100.9 mg plant- 1 to 798.9, 718.9, and 530.4 mg plant- 1 in the MGDA-, GLDA-, and EDTA-amended soils, respectively. Moreover, the amounts of water-extractable, and thereby potentially leachable, Pb and Zn in the post-harvest soil were considerably greater in the soil treated with EDTA than those treated with MGDA and GLDA. Therefore, MGDA and GLDA would be potential alternatives to environmentally-persistent EDTA for enhanced metal phytoextraction from contaminated soils.