Association between Toxoplasma gondii and gestational diabetes with regard to serum leptin and tumor necrosis factor alpha, a preliminary study.

Gestational diabetes (GDM), the onset of any degree of glucose intolerance during pregnancy, increases a wide range of adverse health outcomes for both the mother and the fetus. The aim of the present study was to evaluate the association of Toxoplasma gondii infection with GDM in a case-control study with regard to the levels of leptin and tumor necrosis factor alpha (TNF-α) as two inflammatory biomarkers. Fifty-one pregnant diabetic cases and 109 controls were selected from a prenatal care clinic of a general hospital in Shiraz, southern Iran during July-November 2020. Cases and controls were similar in age, gestational age and number of parturitions. The presence of IgG antibodies against T. gondii, and serum concentrations of leptin and TNF-α were determined by ELISA. Anti-Toxoplasma antibodies were detected in 25 subjects (15.6 %, 95 % CI: 9.9-21.3). Nine (18 %) diabetic cases were infected with Toxoplasma compared to 16 (15 %) healthy controls (P = 0.63). Level of leptin was higher (P = 0.07) while TNF-α was lower in diabetic cases compared to healthy controls (P = 0.08). When subjects were classified according to the combination of GDM and T. gondii, leptin was significantly lower in healthy (non-diabetic, non-infected) subjects compared to diabetics (P = 0.026), and TNF-α was higher in healthy subjects compared to Toxoplasma-infected diabetics (P = 0.032). These findings can be interpreted as both comorbidities being individually associated with increasing serum leptin and decreasing TNF-α concentrations, with modifying effects on each other. The present study opens a new perspective on GDM and its complex pathophysiological mechanism. Future research in this area is needed to better understand the underlying pathway for the development of GDM and the role of T. gondii and inflammatory biomarkers.

Evaluation of delaying effects of different short-term dosage regimens of topical ciprofloxacin on corneal ulcer healing in an avian model.

BACKGROUND: Knowledge on possible delaying effects of topical ciprofloxacin on corneal ulcer healing is scarce in avian patients. OBJECTIVES: The study evaluates effects of different dosage regimens of topical ciprofloxacin on healing of corneal ulcer in an avian model. METHODS: One hundred and fifty adult layers were randomly allocated into two equal categories each consisted of 5 groups (n = 15): 1, negative control (NC, normal cornea); 2, positive control (PC) (birds with experimental corneal ulcer); and 3, 4 and 5, birds with corneal injury that received ciprofloxacin 0.3% topically q6h, q8h and q12h, respectively for 3 (category 1) or 5 days (category 2). Corneas were excised for histopathological evaluation and determination of MMP-9 expression. RESULTS: While no significant difference was observed in daily-measured fluorescein-stained ulcer size among ciprofloxacin-treated birds and PC group in category 1, birds in PC group of category 2 had significantly smaller ulcers as compared to antibiotic-treated birds at the end of experiment (p < 0.01 for all cases). Histopathological evaluations at the end of the experiment showed no significant difference among PC and ciprofloxacin-treated birds of both categories for almost all of the assayed parameters. Over expression of MMP-9 mRNA was observed in PC group after 3 and 5 days of ulcer induction compared to NC groups. Its expression in ciprofloxacin-treated birds of both categories remained close to PC groups. CONCLUSIONS: While ciprofloxacin administration for 3 days does not affect ulcer healing, it delays healing process at the end of 5 days of treatments in an avian model of corneal ulcer injury. This delaying effect is not associated with a drastic change in MMP-9 expression.

Association of Neospora caninum with reproductive performance in dairy cows: A prospective study from Iran.

There is no prospective study from Iran to estimate the direct risk of Neospora caninum for pregnancy loss or reproductive factors. In addition, there is no report in the literature concerning the association of N. caninum with dystocia and sex of calves. Therefore, this study was conducted on a group of dairy cows in a large intensive production system during 2011 to 2013 in southern Iran to evaluate the impact of neosporosis on reproductive performance. A total of 253 cows which were diagnosed as pregnant during the first six months of the study were followed until calving or abortion. Reproductive data were collected and N. caninum serostatus was determined using ELISA. To investigate the association of abortion with N. caninum, survival analysis was performed using Cox proportional hazard model. The N. caninum seroprevalence in the study group was 30.40% (95% CI: 27.40, 36.10). The overall abortion rate was 12.25%, significantly higher in seropositive animals (20.80%) than seronegative ones (8.50%). Results of Cox model showed that serostatus of animal for N. caninum and season had significant associations with abortion (p < 0.01). Neospora caninum did not show significant association with other factors such as dystocia and sex of calves. In conclusion, neosporosis is responsible for 12.00% excess abortion risk in infected group and more than 30.00% of abortions could be preventable by control of Neospora in study population. Therefore, control of N. caninum would reduce the economic losses caused by parasite mainly due to pregnancy loss.