Cirurgia reconstrutiva múltipla como alternativa à amputação de membro para o tratamento do tumor de bainha de nervo periférico em cão - relato de caso

O tumor de bainha de nervo periférico (TBNP) é um neoplasma incomum em cães, cujo tratamento de eleição envolve a remoção cirúrgica completa da neoformação com ampla margem de segurança. Em virtude do seu comportamento infiltrativo, a depender da localização anatômica, recomenda-se a amputação do membro, procedimento mutilante com repercussões clínicas importantes, como alterações deambulatórias, dentre outras. Neste contexto, objetiva-se com esse trabalho relatar o caso de uma cadela sem raça definida, diagnosticada com TBNP em membro pélvico esquerdo e submetida à ressecção completa da neoformação com ampla margem de segurança, seguida de reconstrução cirúrgica do membro, como alternativa à amputação do mesmo. A paciente apresentou 411 dias de sobrevida até o presente momento, e ausência de recidiva tumoral local. Desta forma, sugere-se que a excisão com ampla margem de segurança associada a reconstrução tecidual possa ser uma alternativa para o tratamento de animais com TBNP em localização apendicular.

The malignant peripheral nerve sheath tumor is an uncommon neoplasm in dogs, which treatment includes the complete surgical removal of the neoplasm with broad surgical margins. Due to its infiltrative behavior, limb amputation is generally recommended. However, limb amputation can be considered a mutilating procedure with important clinical consequences, as deambulatory abnormalities. In this context, the aim of this study was to report the case of a mongrel female dog, diagnosed with malignant peripheral nerve sheath tumor in the left pelvic limb. The animal was submitted to a complete removal of the neoplasm with broad surgical margins, followed by surgical reconstruction of the limb, as an alternative to the amputation. The patient presented survival time of 411 days, and absence of tumoral local recurrence. Thus, it is suggested that complete removal of the neoplasm with broad surgical margins, followed by surgical reconstruction of the limb is an alternative for amputation in animal with appendicular presentation of malignant peripheral nerve sheath tumor.

Cirurgia reconstrutiva múltipla como alternativa à amputação de membro para o tratamento do tumor de bainha de nervo periférico em cão - relato de caso

O tumor de bainha de nervo periférico (TBNP) é um neoplasma incomum em cães, cujo tratamento de eleição envolve a remoção cirúrgica completa da neoformação com ampla margem de segurança. Em virtude do seu comportamento infiltrativo, a depender da localização anatômica, recomenda-se a amputação do membro, procedimento mutilante com repercussões clínicas importantes, como alterações deambulatórias, dentre outras. Neste contexto, objetiva-se com esse trabalho relatar o caso de uma cadela sem raça definida, diagnosticada com TBNP em membro pélvico esquerdo e submetida à ressecção completa da neoformação com ampla margem de segurança, seguida de reconstrução cirúrgica do membro, como alternativa à amputação do mesmo. A paciente apresentou 411 dias de sobrevida até o presente momento, e ausência de recidiva tumoral local. Desta forma, sugere-se que a excisão com ampla margem de segurança associada a reconstrução tecidual possa ser uma alternativa para o tratamento de animais com TBNP em localização apendicular.(AU)

The malignant peripheral nerve sheath tumor is an uncommon neoplasm in dogs, which treatment includes the complete surgical removal of the neoplasm with broad surgical margins. Due to its infiltrative behavior, limb amputation is generally recommended. However, limb amputation can be considered a mutilating procedure with important clinical consequences, as deambulatory abnormalities. In this context, the aim of this study was to report the case of a mongrel female dog, diagnosed with malignant peripheral nerve sheath tumor in the left pelvic limb. The animal was submitted to a complete removal of the neoplasm with broad surgical margins, followed by surgical reconstruction of the limb, as an alternative to the amputation. The patient presented survival time of 411 days, and absence of tumoral local recurrence. Thus, it is suggested that complete removal of the neoplasm with broad surgical margins, followed by surgical reconstruction of the limb is an alternative for amputation in animal with appendicular presentation of malignant peripheral nerve sheath tumor.(AU)

Raiva em herbívoros: relato de casos

A raiva é enfermidade importante em herbívoros, sendo zoonose, de evolução rápida e sem tratamento eficaz, exigindo um rigoroso controle profilático. Foram relatados casos positivos para doença atendidos no hospital veterinário da FAEF na cidade de Garça/SP, sendo três bovinos e uma égua. De modo geral apresentaram sinais clínicos característicos da doença e nenhum animal era vacinado contra raiva. Sem respostas aos tratamentos, os animais vieram a óbito, e para diagnóstico, foi feita a coleta de material encefálico desses animais, obtendo todos positivos para raiva. O objetivo do trabalho foi apresentar casos epidemiológicos associado à literatura em relação à raiva.(AU)

Rabies is an important disease in herbivores, being zoonosis, of fast evolution and without effective treatment, demanding a strict prophylactic control. Positive cases for disease were reported at the FAEF veterinary hospital in the city of Garça / SP, with three cattle and one mare. In general, they presented characteristic clinical signs of the disease and no animals were vaccinated against rabies. Without response to the treatments, the animals died, and for diagnosis, the brain was collected from these animals, obtaining all positive for rabies. The objective of this study was to present epidemiological cases associated with the literature regarding rabies.(AU)

Babesiose cerebral em bezerro: relato de caso

A babesiose é causada por hematozoários, como Babesia bovis e Babesia bigemna. Os sinais clínicos encontrados são febre, depressão, anorexia, icterícia, taquicardia, taquipneia, hemoglobinúria e quando há desenvolvimento da babesiose cerebral, os animais infectados por Babesia bovis demonstram sinais neurológicos. O objetivo deste trabalho é relatar caso de babesiose cerebral em bovino jovem, atendido no Hospital Veterinário da Faculdade de Ensino Superior e Formação Integral FAEF. Em neonatos esta enfermidade ocorre com baixa frequência, devido à transferência de imunidade passiva. A necropsia é de essencial importância para o diagnóstico e prevenção desta enfermidade nos animais.(AU)

Babesiosis is caused by hematozoa, such as Babesia bovis and Babesia bigemna. The clinical signs found are fever, depression, anorexia, jaundice, tachycardia, tachypnea, hemoglobinuria and when there is development of cerebral babesiosis, Babesia bovis infected animals show neurological signs. The objective of this work is to report case of cerebral babesiosis in young bovine, attended at the Veterinary Hospital of the Faculty of Higher Education and Integral Training - FAEF. In neonates this disease occurs with low frequency, due to the transference of passive immunity. Necropsy is of essential importance for the diagnosis and prevention of this disease in animals.(AU)

Mieloencefalite protozoária equina

A mieloencefalite protozoária eqüina é uma doença que acomete o sistema nervoso central (SNC) causando uma infecção. O principal agente etiológico é o Sarcocystis neurona e tem como hospedeiro definitivo o Didelphis albiventris (gambá). Há predominância em alguns sinais clínicos como ataxia e paresia. A rápida avaliação do animal e o rápido diagnóstico podem diminuir algumas complicações e diminuir os sinais clínicos presentes nele.(AU)

Equine protozoal myeloencephalitis is a disease that affects the central nervous system (CNS) causing an infection. The main etiological agent is Sarcocystis neuronal and has its definitive host Didelphis albiventris (opossum). There is a predominance of some clinical signs such as ataxia and paresis. Rapid assessment of the animal and rapid diagnosis may decrease some complications and decrease the clinical signs present in it.(AU)

Mieloencefalite protozoária equina

A mieloencefalite protozoária eqüina é uma doença que acomete o sistema nervoso central (SNC) causando uma infecção. O principal agente etiológico é o Sarcocystis neurona e tem como hospedeiro definitivo o Didelphis albiventris (gambá). Há predominância em alguns sinais clínicos como ataxia e paresia. A rápida avaliação do animal e o rápido diagnóstico podem diminuir algumas complicações e diminuir os sinais clínicos presentes nele.

Equine protozoal myeloencephalitis is a disease that affects the central nervous system (CNS) causing an infection. The main etiological agent is Sarcocystis neuronal and has its definitive host Didelphis albiventris (opossum). There is a predominance of some clinical signs such as ataxia and paresis. Rapid assessment of the animal and rapid diagnosis may decrease some complications and decrease the clinical signs present in it.

Babesiose cerebral em bezerro: relato de caso

A babesiose é causada por hematozoários, como Babesia bovis e Babesia bigemna. Os sinais clínicos encontrados são febre, depressão, anorexia, icterícia, taquicardia, taquipneia, hemoglobinúria e quando há desenvolvimento da babesiose cerebral, os animais infectados por Babesia bovis demonstram sinais neurológicos. O objetivo deste trabalho é relatar caso de babesiose cerebral em bovino jovem, atendido no Hospital Veterinário da Faculdade de Ensino Superior e Formação Integral – FAEF. Em neonatos esta enfermidade ocorre com baixa frequência, devido à transferência de imunidade passiva. A necropsia é de essencial importância para o diagnóstico e prevenção desta enfermidade nos animais.

Babesiosis is caused by hematozoa, such as Babesia bovis and Babesia bigemna. The clinical signs found are fever, depression, anorexia, jaundice, tachycardia, tachypnea, hemoglobinuria and when there is development of cerebral babesiosis, Babesia bovis infected animals show neurological signs. The objective of this work is to report case of cerebral babesiosis in young bovine, attended at the Veterinary Hospital of the Faculty of Higher Education and Integral Training - FAEF. In neonates this disease occurs with low frequency, due to the transference of passive immunity. Necropsy is of essential importance for the diagnosis and prevention of this disease in animals.

Raiva em herbívoros: relato de casos

A raiva é enfermidade importante em herbívoros, sendo zoonose, de evolução rápida e sem tratamento eficaz, exigindo um rigoroso controle profilático. Foram relatados casos positivos para doença atendidos no hospital veterinário da FAEF na cidade de Garça/SP, sendo três bovinos e uma égua. De modo geral apresentaram sinais clínicos característicos da doença e nenhum animal era vacinado contra raiva. Sem respostas aos tratamentos, os animais vieram a óbito, e para diagnóstico, foi feita a coleta de material encefálico desses animais, obtendo todos positivos para raiva. O objetivo do trabalho foi apresentar casos epidemiológicos associado à literatura em relação à raiva.

Rabies is an important disease in herbivores, being zoonosis, of fast evolution and without effective treatment, demanding a strict prophylactic control. Positive cases for disease were reported at the FAEF veterinary hospital in the city of Garça / SP, with three cattle and one mare. In general, they presented characteristic clinical signs of the disease and no animals were vaccinated against rabies. Without response to the treatments, the animals died, and for diagnosis, the brain was collected from these animals, obtaining all positive for rabies. The objective of this study was to present epidemiological cases associated with the literature regarding rabies.

Teste sorológico para herpesvírus tipo 5 (chhv-5) em tartarugas-marinhas – revisão de literatura

A fibropapilomatose é uma doença caracterizada pela presença de fibropapilomascutâneos, que podem se distribuir por todo o corpo do animal. Estudos apontam que o herpesvírus associado a fibropapilomatose (ChHV-5) é o causador desta doença, que tem como uma de suas principais características a latência. Os testes sorológicos são uma ferramenta de grande valia para verificar a exposição das tartarugas marinhas ao vírus e, desta forma, trazer dados sobre como o vírus afeta às populações de tartarugas em vida livre. Sendo assim, a padronização do teste para o Herpesvirus tipo 5 (ChHV-5) permitirá a identificação e titulação dos anticorpos mesmo em animais sem sinais clínicos.

Fibropapillomatosis is a disease characterized by the presence of cutaneous fibropapillomas, which can spread throughout the body of the animal. Studies indicate that the herpesvirus associated with fibropapillomatosis (ChHV-5) is the cause of this disease, which has as one of its main characteristics the latency. Serological tests are a valuable tool for verifying the exposure of sea turtles to the virus and thus provide data on how the virus affects turtle populations in free living. Therefore, the standardization of the test for Herpesvirus type 5 (ChHV-5) will allow the identification and titration of the antibodies even in animals without clinical signs.

Teste sorológico para herpesvírus tipo 5 (chhv-5) em tartarugas-marinhas revisão de literatura

A fibropapilomatose é uma doença caracterizada pela presença de fibropapilomascutâneos, que podem se distribuir por todo o corpo do animal. Estudos apontam que o herpesvírus associado a fibropapilomatose (ChHV-5) é o causador desta doença, que tem como uma de suas principais características a latência. Os testes sorológicos são uma ferramenta de grande valia para verificar a exposição das tartarugas marinhas ao vírus e, desta forma, trazer dados sobre como o vírus afeta às populações de tartarugas em vida livre. Sendo assim, a padronização do teste para o Herpesvirus tipo 5 (ChHV-5) permitirá a identificação e titulação dos anticorpos mesmo em animais sem sinais clínicos.(AU)

Fibropapillomatosis is a disease characterized by the presence of cutaneous fibropapillomas, which can spread throughout the body of the animal. Studies indicate that the herpesvirus associated with fibropapillomatosis (ChHV-5) is the cause of this disease, which has as one of its main characteristics the latency. Serological tests are a valuable tool for verifying the exposure of sea turtles to the virus and thus provide data on how the virus affects turtle populations in free living. Therefore, the standardization of the test for Herpesvirus type 5 (ChHV-5) will allow the identification and titration of the antibodies even in animals without clinical signs.(AU)

A glândula pineale sua importancia na reprodução e ciclos circadiano e migratório das aves marinhas - revisão de literatura

Nas aves, os marca-passos circadianos estão localizados no hipotálamo, na glândula pineal e/ou nos os olhos. Esta glândula exerce função de modulação endócrina e regulação de sistemas não endócrinos, quando estes sofrem a ação de alterações ambientais e/ou do próprio organismo. Ela é encarregada de monitorar as estações do ano e sincronizar com elas algumas funções que variam anualmente, como o comportamento reprodutor e migratório das aves. Desta forma, torna-se importante o estudo sobre a sua morfologia e como este conhecimento pode auxiliar na sobrevivência das aves marinhas.(AU)

In birds, circadian pacemakers are located in the hypothalamus, pineal gland and / or eyes. This gland exerts a function of endocrine modulation and regulation of non-endocrine systems, when they undergo environmental changes and / or the body itself. She is in charge of monitoring the seasons and synchronizing with them some functions that vary annually, such as the breeding and migratory behavior of the birds. In this way, it becomes important to study its morphology and how this knowledge can aid in the survival of seabirds.(AU)

A glândula pineale sua importancia na reprodução e ciclos circadiano e migratório das aves marinhas - revisão de literatura

Nas aves, os marca-passos circadianos estão localizados no hipotálamo, na glândula pineal e/ou nos os olhos. Esta glândula exerce função de modulação endócrina e regulação de sistemas não endócrinos, quando estes sofrem a ação de alterações ambientais e/ou do próprio organismo. Ela é encarregada de monitorar as estações do ano e sincronizar com elas algumas funções que variam anualmente, como o comportamento reprodutor e migratório das aves. Desta forma, torna-se importante o estudo sobre a sua morfologia e como este conhecimento pode auxiliar na sobrevivência das aves marinhas.

In birds, circadian pacemakers are located in the hypothalamus, pineal gland and / or eyes. This gland exerts a function of endocrine modulation and regulation of non-endocrine systems, when they undergo environmental changes and / or the body itself. She is in charge of monitoring the seasons and synchronizing with them some functions that vary annually, such as the breeding and migratory behavior of the birds. In this way, it becomes important to study its morphology and how this knowledge can aid in the survival of seabirds.

Ovarian torsion in a primipara female dog in Brazil

Background: Ovarian torsion is a condition which the ovary and the ovarian pedicle twist around the ovary suspensory ligament. No report regarding this condition was reported. The aim of the report was to describe a case of unilateral ovarian torsion in a 2-year-old primipara Golden Retriever dog.Case: A 2-year-old female primipara Golden Retriever dog weighting was referred to the Veterinary Hospital, for purulent vaginal discharge evaluation. The dog was presenting anorexia, weight loss, and intermittent diarrhea and vomiting. A cesarean section was performed nine months before her presentation and the oestrus cycle was recorded six months after the cesarean section. Discomfort was observed during the palpation of the abdomen and purulent vaginal was observed. Vaginal swab was performed and followed by cytological examination. The ultrasonographic examination was consistent with pyometra. The ovariohysterectomy was performed and were observed torsion of the left ovary. The histopathological examination of the left ovary and uterus were consistent with ovary necrosis and pyometra. Ten days after surgery the vaginal discharge had resolved and the sutures were removed. Six months postoperatively the dog revealed no further abnormalities. Grossly, the left ovary had firm consistency, dark red surface and 8 cm diameter. The histopathology examination findings were consistent with a diagnosis of diffuse necrosis of left ovary due to ovarian torsion, and uterine suppurative inflammatory process - pyometra.Discussion: Ovarian torsion is uncommon in small animals, but can be observed high incidence in pregnant female dogs than in non-pregnant ones. The 360º an asynchronous ovarian torsion described in the present case is also an unusual condition in primipara female dogs due to the smaller stretching of the ovary suspensory ligament. The ovarian torsion is considerate an emergency condition due to acute abdominal pain.[...]

Ovarian torsion in a primipara female dog in Brazil

Background: Ovarian torsion is a condition which the ovary and the ovarian pedicle twist around the ovary suspensory ligament. No report regarding this condition was reported. The aim of the report was to describe a case of unilateral ovarian torsion in a 2-year-old primipara Golden Retriever dog.Case: A 2-year-old female primipara Golden Retriever dog weighting was referred to the Veterinary Hospital, for purulent vaginal discharge evaluation. The dog was presenting anorexia, weight loss, and intermittent diarrhea and vomiting. A cesarean section was performed nine months before her presentation and the oestrus cycle was recorded six months after the cesarean section. Discomfort was observed during the palpation of the abdomen and purulent vaginal was observed. Vaginal swab was performed and followed by cytological examination. The ultrasonographic examination was consistent with pyometra. The ovariohysterectomy was performed and were observed torsion of the left ovary. The histopathological examination of the left ovary and uterus were consistent with ovary necrosis and pyometra. Ten days after surgery the vaginal discharge had resolved and the sutures were removed. Six months postoperatively the dog revealed no further abnormalities. Grossly, the left ovary had firm consistency, dark red surface and 8 cm diameter. The histopathology examination findings were consistent with a diagnosis of diffuse necrosis of left ovary due to ovarian torsion, and uterine suppurative inflammatory process - pyometra.Discussion: Ovarian torsion is uncommon in small animals, but can be observed high incidence in pregnant female dogs than in non-pregnant ones. The 360º an asynchronous ovarian torsion described in the present case is also an unusual condition in primipara female dogs due to the smaller stretching of the ovary suspensory ligament. The ovarian torsion is considerate an emergency condition due to acute abdominal pain.[...](AU)

Evaluation of cell proliferation and endometrial thickness of bitches in different periods of diestrus

ABSTRACT This study aimed to determine the histological features of the endometrium of bitches, as well as the cell proliferation at specific moments of diestrus, 10, 20, 30, 40, 50 and 60 days post ovulation, correlating the endometrial thickness with the uterine cell proliferation and the metabolic state (weight, blood glucose and plasma cholesterol) of the animals. Therefore, the right and left uterine horns of 26 clinically healthy bitches submitted to ovariohysterectomy were histologically analyzed 10, 20, 30, 40, 50 and 60 days post ovulation. The hematoxylin-eosin and AgNOR staining techniques were performed. All parameters were evaluated by ANOVA and post-hoc Tukey test (p<0.05). The correlation between endometrial thickness and uterine cell proliferation, weight, blood glucose and plasma cholesterol of animals was observed using the Pearson method (p<0.05). In the present study, it is concluded that endometrial thickness does not differ at any of the moments analyzed in diestrus. The endometrial thickness is not influenced by hormones, weight, blood glucose or serum cholesterol of bitches in this phase of the estrous cycle. However, there is greater cell proliferation in the endometrium at day 40 compared to day 60 post ovulation under the influence of the endocrine profile.

Evaluation of cell proliferation and endometrial thickness of bitches in different periods of diestrus.

This study aimed to determine the histological features of the endometrium of bitches, as well as the cell proliferation at specific moments of diestrus, 10, 20, 30, 40, 50 and 60 days post ovulation, correlating the endometrial thickness with the uterine cell proliferation and the metabolic state (weight, blood glucose and plasma cholesterol) of the animals. Therefore, the right and left uterine horns of 26 clinically healthy bitches submitted to ovariohysterectomy were histologically analyzed 10, 20, 30, 40, 50 and 60 days post ovulation. The hematoxylin-eosin and AgNOR staining techniques were performed. All parameters were evaluated by ANOVA and post-hoc Tukey test (p<0.05). The correlation between endometrial thickness and uterine cell proliferation, weight, blood glucose and plasma cholesterol of animals was observed using the Pearson method (p<0.05). In the present study, it is concluded that endometrial thickness does not differ at any of the moments analyzed in diestrus. The endometrial thickness is not influenced by hormones, weight, blood glucose or serum cholesterol of bitches in this phase of the estrous cycle. However, there is greater cell proliferation in the endometrium at day 40 compared to day 60 post ovulation under the influence of the endocrine profile.

Habronemose cutânea equina

Foi atendida no Hospital Escola de Medicina Veterinária da FAEF uma égua adulta, da raça Manga larga, ao exame clínico geral o paciente apresentava-se aparentemente saudável e ao exame específico foiobservado nódulo cutâneo de aspecto granulomatoso, aparentemente aderido ao tecido subcutâneo, de consistência firme e superfície ulcerada. O animal foi submetido a um procedimento cirúrgico de nodulectomia e o tecido foi encaminhado à análise histopatológica. O diagnóstico foi de dermatite granulomatosa e eosinofílica, associada a fragmentos de parasitas intralesionais, compatível então com quadro de habronemose cutânea. Este trabalho tem como objetivo relatar um caso de habronemose cutânea equina.

A male mare, from the Manga largo breed, was attended at the School of Veterinary Medicine of the FAEF. On general clinical examination the patient was apparently healthy and on the specific examination a granulomatous aspect nodule apparently adhering to the subcutaneous tissue was observed, with consistency Firm and ulcerated surface. The animal underwent a surgical nodulectomy procedure and the tissue was referred for histopathological analysis. The diagnosis was of granulomatous andeosinophilic dermatitis, associated with fragments of intralesional parasites, compatible with cutaneous haemonasal disease. This paper aims to report a case of equine cutaneous habronemias.

EFFECT OF Bifidobacterium animalis ON MICE INFECTED WITH Strongyloides venezuelensis / Efeito da administração de Bifidobacterium animalis sobre a infecção por Stongyloides venezuelensis em camundongos

The administration of viable Bifidobacterium animalis was tested to induce resistance against Strongyloides venezuelensis infection in mice. Effects on parasite burden, worm length, egg output, and intestinal mucosal histology were evaluated. The oral administration of B. animalis, strain 04450B, starting 14 days before the inoculation of nematode larvae significantly decreased the worm burden and egg output. In probiotic treated animals, the percent reduction of adult worms in the intestine was of 33% and the reduction of egg production was of 21%, compared with those of the control group. The duodenum villous height and villous/crypt ratio were significantly higher in probiotic-treated mice, indicating that this group could be experiencing less intestinal damage. The present findings revealed that the administration of B. animalis for the amelioration of host response to nematode infections is biologically plausible and could have some potential for impacting public health. Meanwhile, further study is needed to delineate the nature and identity of the factor(s) involved in these beneficial effects.

Os efeitos da administração de Bifidobacterium animalis viáveis sobre a infecção por Strongyloides venezuelensis foram avaliados em camundongos experimentalmente infectados. Os parâmetros analisados incluíram a carga parasitária, o comprimento dos vermes, a quantidade de ovos eliminados e a histologia da mucosa intestinal. A administração oral da cepa 04450B de B. animalis, iniciada 14 dias antes da inoculação de larvas do nematódeo, foi acompanhada de uma redução significativa do número de vermes que se estabeleceu no intestino e do número de ovos eliminados nas fezes. Nos animais tratados com o probiótico, o percentual de redução de vermes adultos no intestino foi de 33% e da produção de ovos foi de 21%, em comparação com os do grupo controle. O comprimento das vilosidades do duodeno e a relação vilus/cripta foram significativamente maiores nos animais tratados, indicando que nestes animais as lesões intestinais foram mais leves. Os resultados do presente trabalho revelaram que a administração de B. animalis com o propósito de modular a resposta do hospedeiro contra infecções por nematódeos é uma possibilidade biologicamente plausível com impacto potencial em saúde pública. No entanto, são ainda necessários mais estudos para esclarecer os mecanismos de ação destes microrganismos e identificar os fatores envolvidos na produção dos efeitos benéficos.

Effect of Bifidobacterium animalis on mice infected with Strongyloides venezuelensis.

The administration of viable Bifidobacterium animalis was tested to induce resistance against Strongyloides venezuelensis infection in mice. Effects on parasite burden, worm length, egg output, and intestinal mucosal histology were evaluated. The oral administration of B. animalis, strain 04450B, starting 14 days before the inoculation of nematode larvae significantly decreased the worm burden and egg output. In probiotic treated animals, the percent reduction of adult worms in the intestine was of 33% and the reduction of egg production was of 21%, compared with those of the control group. The duodenum villous height and villous/crypt ratio were significantly higher in probiotic-treated mice, indicating that this group could be experiencing less intestinal damage. The present findings revealed that the administration of B. animalis for the amelioration of host response to nematode infections is biologically plausible and could have some potential for impacting public health. Meanwhile, further study is needed to delineate the nature and identity of the factor(s) involved in these beneficial effects.

Biomechanical and histologic analysis in aortic endoprosthesis using fibrin glue.

BACKGROUND: The absence of incorporation between endoprosthesis (EP) and the arterial wall may lead to device migration and endoleaks around the stent graft. Alternatives have been tested aiming to improve this incorporation. Fibrin glue is used in many operating procedures promoting adhesion and tissue regeneration; however, its use to improve EP incorporation by arteries is unknown. OBJECTIVE: The objective of this study was to analyze dislodgement forces needed to extract the EPs implanted in pig aorta, compare different oversizing and fibrin glue injections, and to analyze histologic changes among groups. METHODS: Straight EPs were implanted in the thoracic aorta of pigs using 10% oversizing plus fibrin glue in the interface between the EP and the artery (group 1), using 20% oversizing (group 2), and 10% oversizing (group 3). Fourteen days after the implant, the animals were killed to enable biomechanical analysis of the EP and to verify histologic changes of the aortic wall and its interface with the EP. RESULTS: Group 1 showed a dislodgement force of 21.9 ± 5.3 Newton (N) being higher than the other groups and statistically significant when compared to group 3 (15.6 ± 3.6N), P = .003%. Group 2 had a higher dislodgement force and statistically more significant than group 3 (19.5 ± 7.8N). Histologic analysis showed tissue reaction with inflammatory cells and fibroblasts higher in group 1 and group 2 compared to group 3. CONCLUSION: This study reports a large animal survival model of thoracic aortic stent graft placement by testing the impact of fibrin glue on EP incorporation. Compared to oversizing alone, fibrin glue placed between the stent graft and the arterial wall increases EP incorporation. Additional studies are needed to determine the potential utility of fibrin glue in the setting of human arterial endografts.