Subject(s)
Artifacts , Edetic Acid/adverse effects , Thrombocytopenia/diagnosis , Adult , Diagnostic Errors , Humans , Leukocyte Count , Male , Platelet Count , Thrombocytopenia/bloodSubject(s)
Dysentery, Bacillary/mortality , Adolescent , Adult , Child , Child, Preschool , Female , Humans , Infant , Male , Severity of Illness IndexABSTRACT
OBJECTIVE: Assess the prevalence of asymptomatic carriage of vancomycin-resistant Enterococci in a population of healthy young French subjects. METHODS: Stool samples obtained from 100 persons living in south-eastern France (20 sampling sites) were directly seeded on enriched selective media containing 6 mg/l vancomycin. Bacterial species and their resistant gene were identified with classical methods and multiplex genomic amplification. RESULTS: The incidence of asymptomatic carriage was 17% with a homogeneous geographic distribution of the resistant strains. Nine Enterococcus faecium van A strains and 8 E. gallinarum van C1 strains were isolated. CONCLUSION: These findings demonstrate carriage of vancomycin-resistant Enterococci in a population of young ambulatory subjects in France. The incidence observed was much higher than in an earlier study conducted in France. These results might be explained by a much more sensitive detection technique. Care should be taken to avoid dissemination in hospital settings.
Subject(s)
Enterococcus/drug effects , Vancomycin/pharmacology , Adult , Ambulatory Care/statistics & numerical data , Drug Resistance, Microbial , Enterococcus faecium/drug effects , Feces/microbiology , France , Humans , Male , Microbial Sensitivity Tests , Species SpecificityABSTRACT
Dirofilariasis involving Dirofilaria repens is an exceedingly rare event in France. Most cases have involved subcutaneous lesions but ocular and peri-ocular involvement have been observed. The present report describes a case of dirofilariasis involving the conjunctiva in a 61 year-old-man in France. Onset was characterized by the sudden appearance of painful conjunctival cyst. Clinical examination and laboratory tests did not allow diagnosis. Opening the cyst and extracting the parasite allowed diagnosis and treatment. Microscopic examination identified the parasite as a female Dirofilaria repens. This new case of ocular dirofilariasis emphasizes that this parasitic disease is present and probably underestimated in mainland France. Opening of the conjunctival cyst allowed identification of the parasite and successful treatment without sequels.
Subject(s)
Conjunctivitis/diagnosis , Conjunctivitis/parasitology , Dirofilariasis/diagnosis , Eye Infections, Parasitic/diagnosis , Animals , Dirofilaria/isolation & purification , Dirofilariasis/parasitology , Eye Infections, Parasitic/parasitology , Female , France , Humans , Male , Middle AgedABSTRACT
In the present report, we describe two cases of rare extramedullary relapse (skin, muscle) after allogeneic bone marrow transplantation (BMT). As both patients were male and had received sex mismatched (non T-depleted) BMT, marrow samples were analysed with a Y-chromosome specific probe allowing the sensitive detection of host DNA. The proportion of host DNA remained low in all post BMT samples from these patients, even at the time of extramedullary relapse.
