Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 16 de 16
Filter
1.
Rev Pneumol Clin ; 70(4): 236-9, 2014 Aug.
Article in French | MEDLINE | ID: mdl-24646777

ABSTRACT

The non-invasive ventilation (NIV) is a technique of ventilator support to avoid endotracheal intubation and its potential complications. However, it has some complications that are usually harmless to type of erythema and/or cutaneous ulcerations, mouth or nose dryness, conjunctival irritation and rarely lesions of barotrauma, volotrauma or gastric insufflation with nausea and vomiting. We report the case of a patient who had an unusual complication of NIV: sub mucosa gastro-esophageal pneumatosis associated with subcutaneous emphysema occurring on the second day after one hepatectomy which was settled but complicated with a postoperative pulmonary aspiration syndrome.


Subject(s)
Noninvasive Ventilation/adverse effects , Pneumothorax/etiology , Subcutaneous Emphysema/etiology , Esophageal Diseases/diagnosis , Esophageal Diseases/etiology , Humans , Male , Middle Aged , Pneumothorax/diagnosis , Stomach Diseases/diagnosis , Stomach Diseases/etiology , Subcutaneous Emphysema/diagnosis
2.
Pathol Biol (Paris) ; 61(3): 108-12, 2013 Jun.
Article in French | MEDLINE | ID: mdl-22542426

ABSTRACT

OBJECTIVES: Description of the epidemiological and clinical characteristics of the patients introducing risk factors of invasive candidiasis. Analysis of risk factors for candidiasis invasive and evaluation of the contribution of colonization index (CI) in the diagnosis of the systematic candidiasis in medical intensive care. PATIENTS AND METHODS: Prospective observational study (October 2007 to October 2009). The selected patients present risk factors of system IC candidiasis with an infectious syndrome or clinical signs suggestive of Candida infection and hospitalized more than 48 hours in medical intensive care unit. Pittet's colonization index was calculated at admission and then once a week added to a blood culture. Patients were classified according to level of evidence of Candida infection and the degree of colonization (CI<0.5, CI ≥ 0.5). RESULTS: The study included 100 patients. Mean age of our patients was 55.8 ± 18.2 years with male prevalence. Neurological disease was the most frequent pathology in admission (48%). The most common risk factors were broad-spectrum antibiotics and foreign material. In the various mycology IC specimens, Candida albicans was the most frequent, followed by C. tropicalis, then C. glabrata. The CI was greater than or equal to 0.5 at 53% of the patients, and less than 0.5 in 47% of the cases. Among the patients, 15% developed an invasive candidiasis. In multivariate analysis, the corticosteroid therapy was associated with a high colonisation (IC ≥ 0.5) and neutropenia with a high risk of systemic candidiasis. The positive predictive value of CI was 26%. The negative predictive value was 98%, the sensitivity and specificity was 93% and 48% respectively. CONCLUSION: CI has the advantage to provide a quantified data of the patient's situation in relation to the colonization. But, it isn't helpful with patients having an invasive candidiasis in medical intensive care unit.


Subject(s)
Candida/growth & development , Candidiasis/epidemiology , Candidiasis/microbiology , Intensive Care Units/statistics & numerical data , Adolescent , Adult , Aged , Aged, 80 and over , Child , Colony Count, Microbial , Female , Humans , Male , Middle Aged , Risk Factors , Severity of Illness Index , Young Adult
3.
Rev Pneumol Clin ; 68(3): 199-201, 2012 Jun.
Article in French | MEDLINE | ID: mdl-22677109

ABSTRACT

We report a case of unilateral bronchospasm encountered following an induction of anesthesia of healthy young man with no significant past medical or surgical history. The differential diagnosis and management are discussed. Unilateral bronchospasm was probably caused by topical lidocaine injected with a Laryngojet injector at the vocal cords.


Subject(s)
Anesthetics, Local/adverse effects , Bronchial Spasm/chemically induced , Lidocaine/adverse effects , Diagnosis, Differential , Humans , Intubation, Intratracheal/adverse effects , Male , Middle Aged , Vocal Cords
4.
Nanotechnology ; 23(25): 255101, 2012 Jun 29.
Article in English | MEDLINE | ID: mdl-22652407

ABSTRACT

Recent experiments have shown unambiguously that living cells respond to the nano-topography of surfaces they grow on-specifically, the fate of stem cells grown on nano-porous titania or alumina have been shown to be decided by the pore size. However, most experiments have focused on pore size or pitch. Here we show that in addition to pore size and pitch, the depth of the pores has a profound effect on cell morphology and the arrangement of the actin cytoskeleton.


Subject(s)
Aluminum Oxide/chemistry , Cell Culture Techniques/instrumentation , Cell Physiological Phenomena/physiology , Nanopores/ultrastructure , Nanotechnology/instrumentation , Actin Cytoskeleton/chemistry , Cell Line , Humans , Microscopy, Fluorescence , Porosity , Surface Properties
6.
Ann Fr Anesth Reanim ; 29(4): 274-8, 2010 Apr.
Article in French | MEDLINE | ID: mdl-20117910

ABSTRACT

INTRODUCTION: The reduction of postoperative pain after surgery of inguinal hernia is an objective of lot of studies. The subfasciale infiltration of the wound may be an efficient technique. METHODS: This study was designed as a randomized, double blind, prospective study, comparing two treatment groups: a group infiltrated by bupivacaine (Gr B), and second one infiltrated by a placebo (Gr P). A part of demographic parameters and ASA class, the postoperative pain intensity at rest and at coughing, the morphine consumption and the secondary effects were compared. Patient's satisfaction and postoperative chronic pain at 3 and 6 months were also analyzed. RESULTS: Concerning demographic parameters, ASA class and secondary effects, we didn't find any meaningful difference between the two groups. However, there was a significant reduction of postoperative pain in the bupivacaine group as well at rest as coughing. Gr P patients have more morphine consumption and they were unsatisfied and accused more chronic pain. DISCUSSION: Wound infiltration is still a simple and efficient technique in postoperative pain reduction. With this technique, hernia surgery may become ambulatory.


Subject(s)
Anesthetics, Local/therapeutic use , Bupivacaine/therapeutic use , Hernia, Inguinal/surgery , Pain, Postoperative/drug therapy , Adult , Analgesics, Opioid/administration & dosage , Analgesics, Opioid/therapeutic use , Anesthetics, Local/administration & dosage , Body Temperature , Bupivacaine/administration & dosage , Chronic Disease , Cough/complications , Double-Blind Method , Female , Humans , Injections , Male , Middle Aged , Morphine/administration & dosage , Morphine/therapeutic use , Pain Measurement , Patient Satisfaction , Prospective Studies
7.
Med Mal Infect ; 40(2): 112-4, 2010 Feb.
Article in French | MEDLINE | ID: mdl-19482451

ABSTRACT

Aspergillus fumigatus is a resistant filamentous fungus, common in the environment and pathogenic for human. The most common sites of primary aspergillosis are the maxillary sinus and lungs. Intracranial location is rare and may develop after hematogenous dissemination or contiguity. The invasive pseudotumoral form usually concerns immunodepressed patients. Invasive aspergillosis of the cavum is rare and its diagnosis is difficult. This infection can be fatal because of endocranial involvement and permeabilization of the blood-brain barrier, facilitating other neuromeningeal mycotic infections. We report the case of a 70-year-old diabetic and hypertensive female patient having presented with an invasive aspergillosis of the cavum and endocranial involvement complicated by Candida meningitis.


Subject(s)
Aspergillosis/complications , Aspergillus fumigatus , Candidiasis/complications , Meningitis, Fungal/complications , Aged , Fatal Outcome , Female , Humans
13.
Biophys J ; 89(2): 1120-31, 2005 Aug.
Article in English | MEDLINE | ID: mdl-15923221

ABSTRACT

A new strategy was established to determine the average orientation and dynamics of ergosterol in dimyristoylphosphatidylcholine model membranes. It is based on the analysis of chemical shift anisotropies (CSAs) averaged by the molecular dynamics. Static (13)C CSA tensors were computed by quantum chemistry, using the gauge-including atomic-orbital approach within Hartree-Fock theory. Uniformly (13)C-labeled ergosterol was purified from Pichia pastoris cells grown on labeled methanol. After reconstitution into dimyristoylphosphatidylcholine lipids, the complete (1)H and (13)C assignment of ergosterol's resonances was performed using a combination of magic-angle spinning two-dimensional experiments. Dynamically averaged CSAs were determined by standard side-band intensity analysis for isolated (13)C resonances (C(3) and ethylenic carbons) and by off-magic-angle spinning experiments for other carbons. A set of 18 constraints was thus obtained, from which the sterol's molecular order parameter and average orientation could be precisely defined. The validity of using computed CSAs in this strategy was verified on cholesterol model systems. This new method allowed us to quantify ergosterol's dynamics at three molar ratios: 16 mol % (Ld phase), 30 mol % (Lo phase), and 23 mol % (mixed phases). Contrary to cholesterol, ergosterol's molecular diffusion axis makes an important angle (14 degrees) with the inertial axis of the rigid four-ring system.


Subject(s)
Dimyristoylphosphatidylcholine/chemistry , Ergosterol/chemistry , Lipid Bilayers/chemistry , Magnetic Resonance Spectroscopy/methods , Membrane Fluidity , Models, Chemical , Models, Molecular , Anisotropy , Carbon Isotopes , Computer Simulation , Diffusion , Ergosterol/analysis , Kinetics , Membranes, Artificial , Pichia/metabolism , Protein Conformation
14.
Biochem Educ ; 28(3): 171-173, 2000 May 01.
Article in English | MEDLINE | ID: mdl-10878318

ABSTRACT

Although fluorescence is a powerful and much used tool in Biology there are very few applications for teaching this topic in Biochemistry courses. Some classical experiments in carboxyfluorescein fluorescence are presented here, including recording a fluorescence spectrum, quenching by oxygen, effect of concentration and pH on fluorescence signal, and a Stern-Volmer representation for a dynamic quenching.

15.
J Biomol NMR ; 14(3): 231-9, 1999 Jul.
Article in English | MEDLINE | ID: mdl-10481275

ABSTRACT

Methylotrophic yeast has previously been shown to be an excellent system for the cost-effective production of perdeuterated biomass and for the heterologous expression of membrane receptors. A protocol for the expression of 85% deuterated, functional human mu-opiate receptor was established. For partially deuterated biomass, deuteration level and distribution were determined for fatty acids, amino acids and carbohydrates. It was shown that prior to biosynthesis of lipids and amino acids (and of carbohydrates, to a lower extent), exchange occurs between water and methanol hydrogen atoms, so that 80%-90% randomly deuterated biomass and over-expressed proteins may be obtained using only deuterated water.


Subject(s)
Deuterium , Isotope Labeling/methods , Methanol/metabolism , Pichia/metabolism , Receptors, Opioid, mu/isolation & purification , Biomass , Gas Chromatography-Mass Spectrometry , Humans , Nuclear Magnetic Resonance, Biomolecular , Pichia/genetics , Receptors, Opioid, mu/genetics , Recombinant Proteins/isolation & purification
16.
Res Virol ; 149(6): 363-73, 1998.
Article in English | MEDLINE | ID: mdl-9923012

ABSTRACT

We report the structure and antigenicity of the third variable region (V3) of the HIV2 envelope glycoprotein by the use of linear and cyclic peptides. To this end, a peptide mimicking this region was synthesized and purified, both as an iodoacetamidated linear peptide and a disulphide-bridged cyclic peptide. The cross-reactivity of three monoclonal antibodies (mAbs) produced against the envelope glycoprotein gp140 with the linear and cyclic peptides was tested with ELISA. The results showed that the cyclic peptide is a better ligand for the 3 mAbs 125-F, 125-J and 125-K. The avidity of the mAb/peptide interaction was further analysed by determining the concentration of linear or cyclic peptide leading to 50% inhibition of mAb-peptide complex formation (K0.5). The K0.5 value of mAb 125-F, which displayed the best reactivity with gp140, was estimated to be 5 times higher for the linear (K0.5 = 1.5 x 10(-6) M) than for the cyclic peptide (K0.5 = 3 x 10(-7) M). This indicates a higher affinity of mAb 125-F for the cyclic peptide. mAb 125-J, which exhibited a lower avidity for the gp140 compared to mAb 125-F, had a similar affinity for the cyclic and the linear peptides (K0.5 = 3 x 10(-7) M). mAb 125-K had the lowest reactivity with gp140 and its binding to adsorbed peptide could not be inhibited by the soluble linear or cyclic peptide used up to 10(-5) M. These results suggest that cyclic peptides may have a higher propensity for adopting a native-like structure for the peptide/antibody interaction. Nuclear magnetic resonance experiments at 25 degrees C in phosphate buffer pH 5.4, however, showed that neither peptide displayed a well-defined structure.


Subject(s)
HIV Envelope Protein gp120/immunology , HIV-2/immunology , Peptide Fragments/immunology , Peptides, Cyclic/immunology , Peptides/immunology , Protein Conformation , Animals , Antibodies, Monoclonal/immunology , HIV Antibodies/immunology , HIV Envelope Protein gp120/chemistry , Mice , Peptide Fragments/chemistry , Peptides/chemistry , Peptides, Cyclic/chemistry , Structure-Activity Relationship
SELECTION OF CITATIONS
SEARCH DETAIL
...