A comparison of antimicrobial regimen outcomes and antibiogram development in microbial keratitis: a prospective cohort study in Alexandria, Egypt.

INTRODUCTION: Antimicrobial resistance in microbial keratitis has not been previously explored in Alexandria. We aim to recommend effective therapies through identification of etiological agents, determination of antimicrobial susceptibilities, and comparing outcomes of empiric topical antimicrobials. METHODS: In this 2022 prospective cohort conducted in Alexandria Main University Hospital cornea clinic, antimicrobial susceptibilities of isolated microorganisms from corneal scrapings were detected and antibiograms were developed. Bacterial (BK), fungal (FK), or mixed fungal/bacterial keratitis (MFBK) patients on empiric regimens were compared for ulcer healing, time-to-epithelialization, best-corrected visual acuity, interventions, and complications. RESULTS: The prevalent microorganisms in 93 positive-cultures were coagulase-negative staphylococci (CoNS, 30.1%), Pseudomonas aeruginosa (14%), and Aspergillus spp. (12.9%). CoNS were susceptible to vancomycin (VAN, 100%) and moxifloxacin (MOX, 90.9%). Gram-negative bacteria showed more susceptibility to gatifloxacin (90.9%) than MOX (57.1%), and to gentamicin (GEN, 44.4%) than ceftazidime (CAZ, 11.8%). Methicillin-resistance reached 23.9% among Gram-positive bacteria. Fungi exhibited 10% resistance to voriconazole (VRC). Percentages of healed ulcers in 49 BK patients using GEN + VAN, CAZ + VAN and MOX were 85.7%, 44.4%, and 64.5%, respectively (p = 0.259). Their median time-to-epithelialization reached 21, 30, and 30 days, respectively (log-rank p = 0.020). In 51 FK patients, more ulcers (88.9%) healed with natamycin (NT) + VRC combination compared to VRC (39.1%) or NT (52.6%) (p = 0.036). Their median time-to-epithelialization was 65, 60, and 22 days, respectively (log-rank p < 0.001). The VRC group required more interventions (60.9%) than NT + VRC-treated group (11.1%) (p = 0.018). In 23 MFBK patients, none healed using NT + CAZ + VAN, while 50% healed using VRC + CAZ + VAN (p = 0.052). Regimens had comparable visual outcomes and complications. CONCLUSION: Based on the higher detected susceptibility, we recommend empiric MOX in suspected Gram-positive BK, gatifloxacin in Gram-negative BK, and GEN + VAN in severe BK. Due to better outcomes, we recommend NT + VRC in severe FK. TRIAL REGISTRATION: ClinicalTrials.gov identifier, NCT05655689. Registered December 19, 2022- Retrospectively registered, https://clinicaltrials.gov/ct2/show/NCT05655689?cond=NCT05655689.&draw=2&rank=1.

Small Incision Lenticule Extraction for Postkeratoplasty Myopia and Astigmatism.

Purpose. To evaluate the visual and refractive outcomes after small incision lenticule extraction (SMILE) for treating myopia and myopic astigmatism after penetrating keratoplasty (PKP). Design. Case-series. Methods. Ten eyes of 10 patients with previous PKP and residual myopic astigmatism for whom pentacam imaging and thickness measurements were acceptable for laser vision correction. Manifest refraction (MR), uncorrected distance visual acuity (UDVA), and corrected distance visual acuity (CDVA) were obtained preoperatively and one day, one week, and one, 3, and 6 months postoperatively. Cases were operated on the VisuMax® femtosecond laser platform with 500 kHz repetition rate. Results. The mean correction ratio for spherical errors was 0.84 ± 0.19 D and for the mean refractive spherical equivalent (MRSE) was 0.79 ± 0.13 D. Vector analysis showed a mean astigmatism reduction at the intended axis of 67 ± 25.25%, a correction index of 0.81 ± 0.21, and an overall mean percentage of success of astigmatism surgery of 53 ± 37.9%. The postoperative MRSE was stable throughout the 6-month follow-up period. The efficacy index was 0.93 and the safety index was 1.12. Conclusion. SMILE for correction of post-PKP myopia and astigmatism is effective, safe, and stable with moderate accuracy and predictability. Centration of the treatment within the grafts was easily performed.

Comparison of visual outcomes after variable spot scanning ablation versus wavefront-optimized myopic LASIK.

PURPOSE: To compare the refractive and visual outcomes of variable spot scanning ablation versus the wavefront-optimized ablation for myopia and myopic astigmatism. METHODS: Fifty patients with myopia who underwent LASIK (100 eyes) were divided into two equal groups. Myopic correction performed using the variable spot scanning (VSS) ablation with the VISX STAR S4/IR platform (Abbott Medical Optics, Inc., Santa Ana, CA) (VSS group) and wavefront-optimized (WFO) ablation profile with the WaveLight Allegretto Wave Eye-Q platform (Alcon Laboratories, Inc., Fort Worth, TX) (WFO group). Manifest refraction, uncorrected distance visual acuity, and corrected distance visual acuity were obtained preoperatively and 1 day and 1 and 3 months postoperatively. Wavefront measurement and contrast sensitivity testing were done preoperatively and 3 months postoperatively. RESULTS: Postoperative mean refractive spherical equivalent was -0.14 ± 0.2 diopters for the VSS group and -0.15 ± 0.28 diopters for the WFO group. Forty-eight eyes of the VSS group and 47 eyes of the WFO group were within ±0.5 diopters. Postoperative mean corrected distance visual acuity was 1.05 ± 0.13 for the VSS group and 1.06 ± 0.12 for the WFO group. The postoperative uncorrected distance visual acuity was 1.01 ± 0.16 for the VSS group and 1.01 ± 0.11 for the WFO group. The safety index was 1.12 for the VSS group and 1.06 for the WFO group, whereas the efficacy index was 1.07 for the VSS group and 1.01 for the WFO group. The mean induced positive spherical aberration was 0.041 ± 0.046 µm for the VSS group and 0.195 ± 0.171 µm for the WFO group (P < .001). Mesopic contrast sensitivity testing showed no statistically significant differences between groups at all tested spatial frequencies. CONCLUSIONS: Both VSS and WFO treatments showed similar refractive and visual outcomes. Both induced significant positive spherical aberration, significantly more with WFO.