Toward the improvement of total nitrogen deposition budgets in the United States.

Frameworks for limiting ecosystem exposure to excess nutrients and acidity require accurate and complete deposition budgets of reactive nitrogen (Nr). While much progress has been made in developing total Nr deposition budgets for the U.S., current budgets remain limited by key data and knowledge gaps. Analysis of National Atmospheric Deposition Program Total Deposition (NADP/TDep) data illustrates several aspects of current Nr deposition that motivate additional research. Averaged across the continental U.S., dry deposition contributes slightly more (55%) to total deposition than wet deposition and is the dominant process (>90%) over broad areas of the Southwest and other arid regions of the West. Lack of dry deposition measurements imposes a reliance on models, resulting in a much higher degree of uncertainty relative to wet deposition which is routinely measured. As nitrogen oxide (NOx) emissions continue to decline, reduced forms of inorganic nitrogen (NHx = NH3 + NH4+) now contribute >50% of total Nr deposition over large areas of the U.S. Expanded monitoring and additional process-level research are needed to better understand NHx deposition, its contribution to total Nr deposition budgets, and the processes by which reduced N deposits to ecosystems. Urban and suburban areas are hotspots where routine monitoring of oxidized and reduced Nr deposition is needed. Finally, deposition budgets have incomplete information about the speciation of atmospheric nitrogen; monitoring networks do not capture important forms of Nr such as organic nitrogen. Building on these themes, we detail the state of the science of Nr deposition budgets in the U.S. and highlight research priorities to improve deposition budgets in terms of monitoring and flux measurements, leaf- to regional-scale modeling, source apportionment, and characterization of deposition trends and patterns.

Current-use pesticides and organochlorine compounds in precipitation and lake sediment from two high-elevation national parks in the Western United States.

Current-use pesticides (CUPs) and banned organochlorine compounds (OCCs) were measured in precipitation (snowpack and rain) and lake sediments from two national parks in the Western United States to determine their occurrence and distribution in high-elevation environments. CUPs frequently detected in snow were endosulfan, dacthal, and chlorothalonil in concentrations ranging from 0.07 to 2.4 ng/L. Of the OCCs, chlordane, hexachlorobenzene, and two polychlorinated biphenyl congeners were detected in only one snow sample each. Pesticides most frequently detected in rain were atrazine, carbaryl, and dacthal in concentrations from 3.0 to 95 ng/L. Estimated annual deposition rates in one of the parks were 8.4 microg/m2 for atrazine, 9.9 microg/m2 for carbaryl, and 2.6 microg/m2 for dacthal, of which >85% occurred during summer. p,p'-DDE and p,p'-DDD were the most frequently detected OCCs in surface sediments from lakes. However, concentrations were low (0.12 to 4.7 microg/kg) and below levels at which harmful effects for benthic organisms are likely to be observed. DDD and DDE concentrations in an age-dated sediment core suggest that atmospheric deposition of DDT and its degradates, and possibly other banned OCCs, to high-elevation areas have been decreasing since the 1970s. Dacthal and endosulfan sulfate were present in low concentrations (0.11 to 1.2 microg/kg) and were the only CUPs detected in surface sediments. Both pesticides were frequently detected in snow, confirming that some CUPs entering high-elevation aquatic environments through atmospheric deposition are accumulating in lake sediments and potentially in aquatic biota as well.

Synthesis of disaturated phosphatidylcholine by cholinephosphotransferase in rat lung microsomes.

1. Incubation of rat lung microsomes with cytidine diphospho[methyl-14C]choline resulted in synthesis of radioactively labeled phosphatidylcholine. 2. 10-15% of this phosphatidylcholine appeared to be disaturated species. In similar experiments with rat liver microsomes only 2-3% of the radioactivity was present in the disaturated species. 3. When de novo synthesis was blocked after 5 min by addition of Ca2+ no increase in the proportion of disaturated phosphatidylcholine was observed upon further incubation of lung microsomes. Under these conditions the enzymes involved in a remodeling mechanism, i.e. phospholipase A and acyl-CoA: lysophosphatidyl-choline acyltransferase, remain fully active. 4. Addition of diacylglycerols from egg phosphatidylcholine containing trace amounts of di[1-14C]palmitoyl glycerol resulted in direct incorporation of 14C label into phosphatidylcholine. The rate of phosphatidylcholine synthesis measured from incorporation of di[1-14C]palmitoyl glycerol equalled that observed with labeled CDP choline. 5. These results support the conclusion that disaturated phosphatidylcholine in lung can be formed by direct utilization of disaturated diacylglycerol and is not produced exclusively via remodelling of de novo synthesized unsaturated species.