Comparative Efficacy of Pimephales promelas, Fundulus diaphanus, and Gambusia affinis and Influence of Prey Density for Biological Control of Culex pipiens molestus Larvae.

Larval survival times and density-dependent feeding behavior were evaluated with the use of 2 species of fish native to the northeastern USA (Pimephales promelas and Fundulus diaphanus), and the potentially invasive Gambusia affinis. Each species was provided 10, 20, 30, 45, and 60 4th-stage larvae of Culex pipiens molestus/fish in the laboratory and digital images were recorded to quantify the number of surviving larvae at various intervals. Daily feeding rates were greatest at the highest larval density. These were 49.69 ± 4.07 larvae for P. promelas, 60 larvae for F. diaphanus, and 36.44 ± 6.6 larvae for G. affinis. Survival analysis was used to compare efficacy of each fish species over time. All fish species consumed larvae at similar rates at lower densities, but significant differences occurred at densities of 30-60 larvae/fish. Survival times of larvae at the highest density were 44 ± 7.9 h for P. promelas, 15 ± 3.4 h for F. diaphanus, and 70.6 ±13 h for G. affinis. In order to evaluate feeding rate as a function of prey density, we compared consumption rates 1.5 h after feeding with the use of a 4-parameter logistic model. Fundulus diaphanus and G. affinis feeding aligned with the 4-parameter model, indicating that initial feeding rates for these species increased with prey density to an upper limit (satiation). Pimephales promelas feeding within 1.5 h did not align with this model, suggesting that early feeding rates for this species are not heavily influenced by prey density.

Developmental rate estimation and life table analysis for Halyomorpha halys (Hemiptera: Pentatomidae).

Egg and nymphal development were studied under constant temperatures for the newly introduced pest species, Halyomorpha halys (Stål) (Hemiptera: Pentatomidae). Development was assessed at seven constant temperatures (15-35 degrees C). Development to adult was completed at temperatures between 17 and 33 degrees C, with egg hatch also occurring at 15 degrees C. The relationship between temperature and developmental rate was evaluated using three developmental models. Of the models evaluated, the Briere-1 model was the best fit for the empirical data of egg and total development and for providing accurate values for the temperature threshold. Application of the linear degree-day model estimated 537.63 DD are needed for total development (egg to imaginal ecdysis). An additional 147.65 DD are needed for the preoviposition period of the female. Reproductive parameters were evaluated at 25 degrees C and indicate a median number of 28 eggs per egg mass. Oviposition occurred at 4.32-d intervals, and a female can continue to oviposit throughout its lifespan. H. halys is univoltine in New Jersey and Pennsylvania, but if it spreads to warmer climates in the United States, it could have multiple generations per year.