ABSTRACT
Introduction: The results of working memory (WM) training to improve episodic memory in older people are inconsistent. This inconsistency could be due to the fact that the episodic memory tests used do not share the same cognitive resources as the trained WM task. The aim of this study was to assess whether performance on an episodic memory test will improve only when this test requires controlled processes of retrieval of information from secondary memory or recollection, similar to the processes exercised during WM training. Method: Fifty-five people over 60 years of age participated in the study: 27 were randomly assigned to the experimental group (EG) and the rest to the control group (CG). The EG was trained in complex span tasks. Before and after training, both groups were tested on episodic memory tests (a verbal and a visuospatial recognition test) and WM span tasks (reading, digit and spatial location). Results: ANOVAs revealed a greater improvement of recollection estimates in the EG than in the CG for both verbal recognition (p = 0.023) and visuospatial recognition (p = 0.014). Discussion: Our results provide support for a cognitive mechanism whose shared presence favored transfer from training on a WM task to a test of episodic memory. Consistent with our predictions, training on complex span tasks improved performance on recognition tests only when recall required a controlled search process in secondary memory, or recollection. We therefore stress the importance of identifying other cognitive resources that are susceptible to transfer from a training task to other untrained tasks. A better understanding of the phenomenon of transfer is crucial for the design of increasingly effective intervention programs for older people.
ABSTRACT
Flap procedures are complex surgical tools widely used in reconstructive surgery. Flap ischemia is one of the most dangerous complications, both during the surgical procedure and during the patient's recovery, which can quickly lead to tissue necrosis (flap loss) with serious medical and psychological consequences. Today, bedside clinical assessment remains the gold standard for flap monitoring, but timely detection of flap ischemia is a difficult and challenging task, so auxiliary techniques are needed to support flap monitoring. Here we present a prototype of a new optical diagnostic tool, based on visible light absorption in diffuse reflectance spectroscopy, for non-invasive, continuous, real-time monitoring of flaps. The proposed approach is assessed by monitoring flap ischemic scenarios induced on pig animal models. The results obtained support that the proposed approach has great potential, not only for prompt detection of ischemia (in seconds), but also for clear differentiation between an arterial occlusion and venous occlusion.
Subject(s)
Arterial Occlusive Diseases , Plastic Surgery Procedures , Humans , Swine , Animals , Surgical Flaps , Ischemia/diagnosis , Ischemia/etiology , Arterial Occlusive Diseases/complications , Postoperative ComplicationsABSTRACT
We present a novel near-infrared spectroscopy technique based on Dual-Comb optical interrogation (DC-NIRS) applied to dispersive media. The technique recovers the frequency response of the medium under investigation by sampling its spectral response in amplitude and phase. The DC-NIRS reference and sample signals are generated using electro-optic modulation which offers a cost-effective, integrable solution while providing high adaptability to the interrogated medium. A careful choice of both line spacing and optical span of the frequency comb ensures that the retrieved information enables the reconstruction of the temporal impulse response of the medium, known as the diffuse-time-of-flight (DTOF), to obtain its optical properties with a 70 µs temporal resolution and 32 ps photon propagation delay resolution. Furthermore, the DC-NIRS technique also offers enhanced penetration due to noiseless optical amplification (interferometric detection). The presented technique was demonstrated on a static bio-mimetic phantom of known optical properties reproducing a typical brain's optical response. The DTOF and optical properties of the phantom were measured, showing the capabilities of this new technique on the estimation of absolute optical properties with a deviation under 3%. Compared to current technologies, our DC-NIRS technique provides enhanced temporal resolution, spatial location capabilities, and penetration depth, with an integrable and configurable cost-effective architecture, paving the way to next-generation, non-invasive and portable systems for functional brain imaging, and brain-computer interfaces, among other. The system is patent pending PCT/ES2022/070176.
ABSTRACT
This study aims to analyze the effects of teacher enthusiasm and type of text on student motivation and achievement. The participants were 369 elementary school students. We used four videos showing a teacher presenting two texts (narrative or descriptive) in two conditions of enthusiasm (high or neutral). A MANOVA revealed additive effects due to enthusiasm and text type on motivation and achievement, but no interaction. Mediation analyzes indicated that enthusiasm showed direct and indirect effects through motivation only for descriptive text. Therefore, the motivational mediation between teacher enthusiasm and student achievement could be especially important when the text is descriptive.
ABSTRACT
In this work, we present a multipurpose photonic integrated circuit capable of generating multiheterodyne complex Dual-Combs (DC) THz signals. Our work focuses on translating the functionality of an electro-optic tunable DC system into a photonic chip employing standard building blocks to ensure the scalability and cost efficiency of the integrated device. The architecture we analyze for integration is based on three stages: a seed comb, a mode selection stage and a DC stage. This final DC stage includes a frequency shifter, a key element to improve the final detection of the THz signals and obtain real-time operation. This investigation covers three key aspects: (1) a solution for comb line selection on GHz spaced combs using OIL or OPLL on photonic chips is studied and evaluated, (2) a simple and versatile scheme to produce a frequency shift using the double sideband suppressed carrier modulation technique and an asymmetric Mach Zehnder Interferometer to filter one of the sidebands is proposed, and (3) a multipurpose architecture that can offer a versatile effective device, moving from application-specific PICs to general-purpose PICs. Using the building blocks (BBs) available from an InP-based foundry, we obtained simulations that offer a high-quality Dual-Comb frequency shifted signal with a side mode suppression ratio around 21 dB, and 41 dB after photodetection with an intermediate frequency of 1 MHz. We tested our system to generate a Dual-Comb with 10 kHz of frequency spacing and an OOK modulation with 5 Gbps which can be down-converted to the THz range by a square law detector. It is also important to note that the presented architecture is multipurpose and can also be applied to THz communications. This design is a step to enable a commercial THz photonic chip for multiple applications such as THz spectroscopy, THz multispectral imaging and THz telecommunications and offers the possibility of being fabricated in a multi-project wafer.
ABSTRACT
In this Letter, we present and experimentally validate the first direct hyperspectral dual-comb gas imaging system operating in the mid-infrared region. This method provides an unmatched combination of super-fine spectral characterization and high temporal resolution without the need for thermal contrast between the target molecules and the background. In a proof-of-concept experiment, the system has allowed us to perform precision hyperspectral imaging of butane in the 3.4 µm band with a time resolution of 1 s.
ABSTRACT
In this paper, a terahertz hyperspectral imaging architecture based on an electro-optic terahertz dual-comb source is presented and demonstrated. In contrast to single frequency sources, this multi-heterodyne system allows for the characterization of the whole spectral response of the sample in parallel for all the frequency points along the spectral range of the system. This hence provides rapid, highly consistent results and minimizes measurement artifacts. The terahertz illumination signal can be tailored (in spectral coverage and resolution) with high flexibility to meet the requirements of any particular application or experimental scenario while maximizing the signal-to-noise ratio of the measurement. Besides this, the system provides absolute frequency accuracy and a very high coherence that allows for direct signal detection without inter-comb synchronization mechanisms, adaptive acquisition, or post-processing. Using a field-effect transistor-based terahertz resonant 300 GHz detector and the raster-scanning method we demonstrate the two-dimensional hyperspectral imaging of samples of different kinds to illustrate the remarkable capabilities of this innovative architecture. A proof-of-concept demonstration has been performed in which tree leaves and a complex plastic fragment have been analyzed in the 300 GHz range with a frequency resolution of 10 GHz.
ABSTRACT
A quantum cascade laser-based sensor for ambient air monitoring is presented and five gases, affecting the air quality, can be quantified. The light sources are selected to measure CO, NO, NO2, N2O and SO2. The footprint of the measurement setup is designed to fit in two standard 19" rack (48 cm × 65 cm) with 4 height units (18 cm) whereas one is holding the optical components and the other one contains the electronics and data processing unit. The concentrations of the individual analytes are measured using 2f-Wavelength Modulation Spectroscopy (2f-WMS) and a commercially available multipass gas cell defines the optical path. In addition, CO can also be measured with a dispersion-based technique, which allows one to cover a wider concentration range than 2f-WMS. The performance of this prototype has been evaluated in the lab and detection limits in the range of 1ppbv have been achieved. Finally, the applicability of this prototype for ambient air monitoring is shown in a five-week measurement campaign in cooperation with the Municipal Department for Environmental Protection (MA 22) of Vienna, Austria.
ABSTRACT
Plants harbor a diversity of microorganisms constituting the plant microbiome. Many bioinoculants for agricultural crops have been isolated from plants. Nevertheless, plants are an underexplored niche for the isolation of microorganisms with other biotechnological applications. As a part of a collection of canola endophytes, we isolated strain CDVBN77T. Its genome sequence shows not only plant growth-promoting (PGP) mechanisms, but also genetic machinery to produce secondary metabolites, with potential applications in the pharmaceutical industry, and to synthesize hydrolytic enzymes, with potential applications in biomass degradation industries. Phylogenetic analysis of the 16S rRNA gene of strain CDVBN77T shows that it belongs to the genus Microvirga, its closest related species being M. aerophila DSM 21344T (97.64% similarity) and M. flavescens c27j1T (97.50% similarity). It contains ubiquinone 10 as the predominant quinone, C19:0 cycloω8c and summed feature 8 as the major fatty acids, and phosphatidylcholine and phosphatidylethanolamine as the most abundant polar lipids. Its genomic DNA G+C content is 62.3 (mol %). Based on phylogenetic, chemotaxonomic, and phenotypic analyses, we suggest the classification of strain CDVBN77T within a new species of the genus Microvirga and propose the name Microvirga brassicacearum sp. nov. (type strain CDVBN77T = CECT 9905T = LMG 31419T).
ABSTRACT
Diabetes is a very complex condition affecting millions of people around the world. Its occurrence, always accompanied by sustained hyperglycemia, leads to many medical complications that can be greatly mitigated when the disease is treated in its earliest stage. In this paper, a novel sensing approach for the early non-invasive detection and monitoring of sustained hyperglycemia is presented. The sensing principle is based on millimeter-wave transmission spectroscopy through the skin and subsequent statistical analysis of the amplitude data. A classifier based on functional principal components for sustained hyperglycemia prediction was validated on a sample of twelve mice, correctly classifying the condition in diabetic mice. Using the same classifier, sixteen mice with drug-induced diabetes were studied for two weeks. The proposed sensing approach was capable of assessing the glycemic states at different stages of induced diabetes, providing a clear transition from normoglycemia to hyperglycemia typically associated with diabetes. This is believed to be the first presentation of such evolution studies using non-invasive sensing. The results obtained indicate that gradual glycemic changes associated with diabetes can be accurately detected by non-invasively sensing the metabolism using a millimeter-wave spectral sensor, with an observed temporal resolution of around four days. This unprecedented detection speed and its non-invasive character could open new opportunities for the continuous control and monitoring of diabetics and the evaluation of response to treatments (including new therapies), enabling a much more appropriate control of the condition.
Subject(s)
Blood Glucose/isolation & purification , Diabetes Mellitus, Experimental/diagnosis , Hyperglycemia/diagnosis , Spectrum Analysis/methods , Animals , Diabetes Mellitus, Experimental/metabolism , Humans , Hyperglycemia/metabolism , MiceABSTRACT
Melinis minutiflora is an invasive species that threatens the biodiversity of the endemic vegetation of the campo rupestre biome in Brazil, displacing the native vegetation and favouring fire spread. As M. minutiflora invasion has been associated with a high nitrogen (N) demand, we assessed changes in N cycle under four treatments: two treatments with contrasting invasion levels (above and below 50%) and two un-invaded control treatments with native vegetation, in the presence or absence of the leguminous species Periandra mediterranea. This latter species was considered to be the main N source in this site due to its ability to fix N2 in association with Bradyrhizobia species. Soil proteolytic activity was high in treatments with P. mediterranea and in those severely invaded, but not in the first steps of invasion. While ammonium was the N-chemical species dominant in plots with native species, including P.mediterranea, soil nitrate prevailed only in fully invaded plots due to the stimulation of the nitrifying bacterial (AOB) and archaeal (AOA) populations carrying the amoA gene. However, in the presence of P. mediterranea, either in the beginning of the invasion or in uninvaded plots, we observed an inhibition of the nitrifying microbial populations and nitrate formation, suggesting that this is a biotic resistance strategy elicited by P. mediterranea to compete with M. minutiflora. Therefore, the inhibition of proteolytic activity and the nitrification process were the strategies elicited by P.mediterranea to constrain M.munitiflora invasion.
Subject(s)
Fabaceae/metabolism , Introduced Species , Nitrogen Fixation , Poaceae/physiology , Soil/chemistry , Archaea/metabolism , Bacteria/metabolism , Fabaceae/microbiology , Fabaceae/physiology , NitrificationABSTRACT
This article presents the first implementation and the experimental characterization of a thermal infrared wavelength modulation laser heterodyne radiometer (WM-LHR) based on an external cavity quantum cascade laser. This novel WM-LHR system has demonstrated calibration-free operation, a superior signal to noise ratio and, more importantly, has opened the door for cost-efficient wide spectral range laser heterodyne radiometry in the near future.
ABSTRACT
We investigate the high frequency modulation characteristics of mid-infrared surface-emitting ring and edge-emitting ridge quantum cascade lasers (QCLs). In particular, a detailed comparison between circular ring devices and ridge-QCLs from the same laser material, which have a linear waveguide in a "Fabry-Pérot (FP) type" cavity, reveals distinct similarities and differences. Both device types are single-mode emitting, based on either 2 nd- (ring-QCL) or 1 st-order (ridge-QCL) distributed feedback (DFB) gratings with an emission wavelength around 7.56 µm. Their modulation characteristics are investigated in the frequency-domain using an optical frequency-to-amplitude conversion technique based on the ro-vibrational absorptions of CH 4. We observe that the amplitude of frequency tuning Δf over intensity modulation index m as function of the modulation frequency behaves similarly for both types of devices, while the ring-QCLs typically show higher values. The frequency-to-intensity modulation (FM-IM) phase shift shows a decrease starting from â¼72 ∘ at a modulation frequency of 800 kHz to about 0 ∘ at 160 MHz. In addition, we also observe a quasi single-sideband (qSSB) regime for modulation frequencies above 100 MHz, which is identified by a vanishing -1 st-order sideband for both devices. This special FM-state can be observed in DFB QCLs and is in strong contrast to the behavior of regular DFB diode lasers, which do not achieve any significant sideband suppression. By analyzing these important high frequency characteristics of ring-QCLs and comparing them to ridge DFB-QCLs, it shows the potential of intersubband devices for applications in e.g. novel spectroscopic techniques and highly-integrated and high-bitrate free-space data communication. In addition, the obtained results close an existing gap in literature for high frequency modulation characteristics of QCLs.
ABSTRACT
An absolute-frequency terahertz (THz) dual-frequency comb spectrometer based on electro-optic modulators for tunable, high-resolution, and real-time rapid acquisition is presented. An optical line of a master frequency comb (filtered via optical injection locking) serves as the seed to electro-optically generate a pair of new frequency combs (probe and local oscillator). Photomixing both combs with another coherent line from the same original master comb generates a narrow linewidth THz dual-comb with teeth frequencies that can be referenced to a radio-frequency standard. The system is validated with a proof-of-principle measurement of a microwave filter in the W-band.
ABSTRACT
Phaseolus vulgaris and Lens culinaris are two legumes with different distribution centers that were introduced in Spain at different times, but in some regions L. culinaris has been traditionally cultivated and P. vulgaris did not. Here we analysed the rhizobia isolated from nodules of these two legumes in one of these regions. MALDI-TOF MS analysis showed that all isolated strains matched with Rhizobium laguerreae and the phylogenetic analysis of rrs, atpD and recA genes confirmed these results. The phylogenetic analysis of these core genes allowed the differentiation of several groups within R. laguerreae and unexpectedly, strains with housekeeping genes identical to that of the type strain of R. laguerreae presented some differences in the rrs gene. In some strains this gene contains an intervening sequence (IVS) identical to that found in Rhizobium strains nodulating several legumes in different geographical locations. The atpD, recA and nodC genes of all isolated strains clustered with those of strains nodulating L. culinaris in its distribution centers, but not with those nodulating P. vulgaris in theirs. Therefore, all these strains belong to the symbiovar viciae, including those isolated from P. vulgaris, which in the studied region established effective symbiosis with the common endosymbiont of L. culinaris, instead to with its common endosymbiont, the symbiovar phaseoli. These results are particularly interesting for biogeography studies, because they showed that, due its high promiscuity degree, P. vulgaris is able to establish symbiosis with local symbiovars well established in the soil after centuries of cultivation with other legumes.
Subject(s)
Phaseolus/microbiology , Phylogeny , Rhizobium/classification , Bacterial Typing Techniques , DNA, Bacterial/genetics , Genes, Bacterial , Rhizobium/isolation & purification , Root Nodules, Plant/microbiology , Sequence Analysis, DNA , Soil Microbiology , SpainABSTRACT
A novel method is proposed for improving the performance of traditional laser heterodyne radiometry. The technique, which is based on the use of a wavelength modulated local oscillator laser, provides baseline-free spectra, lower limits of detection, and better precision and consistency than the conventional approach. This tool could, therefore, boost the accuracy of current terrestrial and planetary atmospheric studies.
ABSTRACT
Electro-optic dual-comb spectrometers have proved to be a promising technology for sensitive, high-resolution and rapid spectral measurements. Electro-optic combs possess very attractive features like simplicity, reliability, bright optical teeth, and typically moderate but quickly tunable optical spans. Furthermore, in a dual-comb arrangement, narrowband electro-optic combs are generated with a level of mutual coherence that is sufficiently high to enable optical multiheterodyning without inter-comb stabilization or signal processing systems. However, this valuable tool still presents several limitations; for instance, on most systems, absolute frequency accuracy and long-term stability cannot be guaranteed; likewise, interferometer-induced phase noise restricts coherence time and limits the attainable signal-to-noise ratio. In this paper, we address these drawbacks and demonstrate a cost-efficient absolute electro-optic dual-comb instrument based on a frequency stabilization mechanism and a novel adaptive interferogram acquisition approach devised for electro-optic dual-combs capable of operating in real-time. The spectrometer, completely built from commercial components, provides sub-ppm frequency uncertainties and enables a signal-to-noise ratio of 10000 (intensity noise) in 30 seconds of integration time.
ABSTRACT
The infection of legume plants by rhizobia is tightly regulated to ensure accurate bacterial penetration, infection, and development of functionally efficient nitrogen-fixing root nodules. Rhizobial Nod factors (NF) have key roles in the elicitation of nodulation signaling. Infection of white clover roots also involves the tightly regulated specific breakdown of the noncrystalline apex of cell walls in growing root hairs, which is mediated by Rhizobium leguminosarum bv. trifolii cellulase CelC2. Here, we have analyzed the impact of this endoglucanase on symbiotic signaling in the model legume Medicago truncatula. Ensifer meliloti constitutively expressing celC gene exhibited delayed nodulation and elicited aberrant ineffective nodules, hampering plant growth in the absence of nitrogen. Cotreatment of roots with NF and CelC2 altered Ca2+ spiking in root hairs and induction of the early nodulin gene ENOD11. Our data suggest that CelC2 alters early signaling between partners in the rhizobia-legume interaction.
Subject(s)
Medicago truncatula/drug effects , Medicago truncatula/microbiology , Plant Root Nodulation/physiology , Rhizobiaceae/metabolism , Signal Transduction/drug effects , beta-Glucosidase/metabolism , Medicago truncatula/metabolism , Plant Root Nodulation/drug effects , SymbiosisABSTRACT
The growing interest in a healthy lifestyle and in environmental protection is changing habits regarding food consumption and agricultural practices. Good agricultural practice is indispensable, particularly for raw vegetables, and can include the use of plant probiotic bacteria for the purpose of biofertilization. In this work we analysed the probiotic potential of the rhizobial strain PEPV40, identified as Rhizobium laguerreae through the analysis of the recA and atpD genes, on the growth of spinach plants. This strain presents several in vitro plant growth promotion mechanisms, such as phosphate solubilisation and the production of indole acetic acid and siderophores. The strain PEPV40 produces cellulose and forms biofilms on abiotic surfaces. GFP labelling of this strain showed that PEPV40 colonizes the roots of spinach plants, forming microcolonies typical of biofilm initiation. Inoculation with this strain significantly increases several vegetative parameters such as leaf number, size and weight, as well as chlorophyll and nitrogen contents. Therefore, our findings indicate, for the first time, that Rhizobium laguerreae is an excellent plant probiotic, which increases the yield and quality of spinach, a vegetable that is increasingly being consumed raw worldwide.
Subject(s)
Probiotics , Rhizobium/physiology , Spinacia oleracea/growth & development , Spinacia oleracea/microbiology , Biofilms , Cellulose/biosynthesis , Phenotype , Phylogeny , Plant Roots/microbiology , Rhizobium/classification , Seedlings/microbiologyABSTRACT
Information received by cancer patients has gained importance in recent decades. The aim of this study was to evaluate the perception of information received by oncological patients in a radiotherapy department and to measure the importance of the other information sources. A cross-sectional study was conducted, evaluating patients who received radiotherapy. All the patients were asked two questionnaires: the EORTC QLQ-INFO26 module evaluating their satisfaction with received information, and a questionnaire analyzing other sources of information search. One hundred patients between 27 and 84 years were enrolled. Breast cancer (26 %) was the commonest cancer. Patients felt better informed about the medical tests and secondly about the performed treatment. The younger patients were those who were more satisfied with the information received and patients with no formal education felt less satisfied, with statistically significant differences. Patients did not seek external information; at the most, they asked relatives and other people with cancer. Patients were satisfied with the received information, although a high percentage would like more information. In general, patients did not search for external information sources. Age and educational level seem to influence in the satisfaction with the received information.