ABSTRACT
Authors studied the activities of Na+-K+-ATP-ase and Mg++-ATP-ase as indicators of lipid peroxidation on rat brain plasmamembrane and microsomal fraction. The CH 402 (Sodium(2,2-dimethyl-1,2-dihydroquinoline-4-yl)methane sulfonate) a synthetic, water soluble, non toxic dihydroquinoline type antioxidant proved to be effective in decreasing the membrane damage caused by ascorbic acid induced lipid peroxidation. The CH 402 did not inhibit the Na+-K+-ATP-ase and Mg++-ATP-ase activities even at a concentration of 10(-3) mol/l.
Subject(s)
Antioxidants/pharmacology , Ascorbic Acid/pharmacology , Brain/enzymology , Ca(2+) Mg(2+)-ATPase/metabolism , Hydroxyquinolines/pharmacology , Sodium-Potassium-Exchanging ATPase/metabolism , Animals , Brain/drug effects , Dose-Response Relationship, Drug , Hydroxyquinolines/administration & dosage , Lipid Peroxidation/drug effects , Male , Rats , Rats, Inbred Strains , Subcellular Fractions/drug effects , Subcellular Fractions/enzymology , Time FactorsABSTRACT
The antiatherosclerotic profile of nicotinic acid and of its new water-insoluble derivative was studied in an 8-day experiment in rats. Both drugs lowered plasma triglyceride levels significantly, while other lipoprotein parameters were unaffected. Circulating immune complex levels were decreased with lysosomal membrane permeability by both drugs. Glunicate proved to be a powerful antioxidant with regard to enzymatic lipid peroxidation when studied in the liver microsomal system. The relevance of these findings to the antiatherosclerotic effect of the drugs and the biological significance of antioxidant treatment is discussed. On the basis of these data glunicate seems to be a promising new therapeutic tool against atherosclerosis and merits further study.
