Dihydromaltophilin; a novel fungicidal tetramic acid containing metabolite from Streptomyces sp.

A new tetramic acid containing metabolite, A90931a has been isolated from Streptomyces sp., along with a second factor (A90931b) recently described and known as maltophilin. The structures were determined from spectroscopic data of the isolates and their acetylated products. A90931a was spectroscopically identical to the previously described antibiotic TAN-883b whose structure was not reported. A90931a and A90931b exhibit fungicidal activity against the grape pathogen Plasmopara viticola. Due to its similarity to maltophilin, A90931a has been called dihydromaltophilin.

An alternative approach to acute cholecystitis. Percutaneous cholecystostomy and interval laparoscopic cholecystectomy.

BACKGROUND: The mainstay of therapy for acute cholecystitis is cholecystectomy, which has a mortality of 5-30% in high-risk patients such as the elderly or critically ill. An alternative treatment option in patients suffering from acute cholecystitis with contraindications to emergency surgery is percutaneous cholecystostomy (PC) followed by interval laparoscopic cholecystectomy. Percutaneous cholecystostomy yields 10-12% mortality in high-risk patients and is therefore a safe temporizing measure, allowing delayed, elective cholecystectomy when the patient is in better condition for surgery. METHODS: Hospital charts and radiology films were reviewed for all 50 patients who underwent PC for acute cholecystitis between January 1990 and September 1993. Most patients were high risk for emergency cholecystectomy by virtue of their critical illness or underlying medical condition. Twenty-five patients went on to have interval cholecystectomies. We recorded whether they underwent laparoscopic or open cholecystectomy, as elective or emergency procedures, and we recorded direct complications, mortality, and postoperative length of hospital stay. RESULTS: Relief of symptoms occurred within 48 h of PC in 90% of patients, and two patients had complications of PC. Laparoscopic cholecystectomy was attempted in 13 patients and competed in nine. Four patients (31%) required conversion from laparoscopic to open cholecystectomies due to extensive adhesions (3) or bleeding (1). Three patients had direct complications of laparoscopic cholecystectomy. There was no mortality or major bile duct injury. CONCLUSION: Percutaneous cholecystostomy followed by interval laparoscopic cholecystectomy is a safe, minimally invasive approach which can be employed safely in the critically ill patient when contraindications to emergency surgery exist.

High resolution endoluminal ultrasonography in the staging of esophageal carcinoma.

Small catheters containing 20 MHz transducers have recently become available for high resolution endoluminal ultrasound. We report our early use of this technique to image and stage esophageal carcinoma. Fifteen patients undergoing high resolution endoluminal ultrasonography for suspected esophageal carcinoma were studied. Twelve of these patients also underwent computed tomography and pathologic correlation was available in seven. Satisfactory esophageal examination was possible in 14 of 15 patients. Of those with pathologic correlation, the depth of tumor invasion was correctly staged by high resolution endoluminal ultrasonography in six of seven patients and by computed tomography in only three of seven patients. Lymph node assessment correlated poorly with pathologic findings for both high resolution endoluminal ultrasound and computed tomography. With the increasing use of preoperative radiation therapy, we believe these early results predict a potential role for high resolution endoluminal ultrasonography in the staging of esophageal carcinoma.

Sonographic assessment of infrarenal inferior vena caval dimensions.

The dimensions of the infrarenal inferior vena cava during quiet respiration, single leg lifting, and breath-holding were assessed using sonography in 156 patients. Sonographic assessment of infrarenal inferior vena caval dimensions was feasible in 69% of patients. Measurements during breath-holding were significantly greater than during quiet respiration (P < 0.001) and leg lifting (P < 0.005), although in approximately one quarter of the patients the mean calculated diameter was greatest during quiet respiration. we conclude that sonographic assessment of infrarenal inferior vena caval dimensions is feasible, but it should be performed during quiet respiration and breath-holding to allow for variation with different respiratory maneuvers.

Peritoneal abscesses due to bowel perforation: effect of extent on outcome after percutaneous drainage.

PURPOSE: To determine the effect of extent of peritoneal contamination in enteric abscesses on final outcome and duration of percutaneous drainage. PATIENTS AND METHODS: Results were retrospectively reviewed for 11 patients with varying degrees of peritoneal contamination secondary to subacute bowel perforation who were primarily treated with percutaneous drainage. Stepwise linear regression analysis of duration of drainage was performed with use of patient age and immune status, the site of bowel perforation, and the number of peritoneal compartments involved in the resultant contamination as independent variables. RESULTS: In 10 of 11 patients (91%) treatment of the resultant intraperitoneal collections with percutaneous drainage was successful irrespective of the extent of peritoneal contamination. There was no correlation between duration of drainage and extent of peritoneal contamination but good correlation with patient age and site of bowel perforation (r = 0.82, P = .02). CONCLUSION: In patients with enteric abscesses due to subacute bowel perforation, the duration of drainage and final outcome after percutaneous drainage are independent of the extent of peritoneal contamination.

Imaging and intervention in abdominal emergencies.

While plain abdominal radiographs retain an essential place in acute abdominal emergencies, the dramatic advances in ultrasound, computed tomography and magnetic resonance imaging witnessed over the past two decades have revolutionized the practice of emergency medicine. Cross-sectional imaging techniques now play a key role in evaluating patients with abdominal emergencies, and allow confident diagnoses to be made in an ever increasing proportion of these patients. Unnecessary laparotomies are now uncommon, and a wide array of interventional radiological techniques can be used to treat abdominal emergencies without surgery.

Effect of hyoscine butylbromide on gastroesophageal reflux in barium studies of the upper gastrointestinal tract.

Hyoscine butylbromide (Buscopan) is an intravenously administered hypotonic agent that significantly reduces pressure in the lower esophageal sphincter and may therefore artificially induce gastroesophageal reflux during barium examination of the upper gastrointestinal tract. This study was performed to test this hypothesis. The presence or absence and severity of gastroesophageal reflux before and after intravenous injection of 20 mg Buscopan were evaluated in 112 consecutive patients undergoing biphasic upper gastrointestinal examination. Gastroesophageal reflux was seen in 49 (44%) of the patients. Reflux was evident both before and after the injection of Buscopan in 35 (31%) of the patients, before injection only in 10 (9%) and after injection only in 4 (4%). The magnitude of reflux after injection of Buscopan was reduced or the same in 108 of the patients (96%) and increased in 4 (4%). There was no significant difference in the overall occurrence (p = 0.41) or degree (p = 0.81) of gastroesophageal reflux before and after injection of Buscopan (chi 2 test). The authors conclude that the routine use of Buscopan is unlikely to spuriously increase the frequency or degree of gastroesophageal reflux observed on upper gastrointestinal barium studies.

Radiologically guided percutaneous cholecystostomy for acute cholecystitis: long-term outcome in 50 patients.

To assess the long-term outcome in patients with acute cholecystitis treated initially by percutaneous cholecystostomy, the authors reviewed the medical and radiology records of all such patients treated at their hospital from January 1990 to September 1993. Of the 50 patients, 29 had calculous and 21 had acalculous cholecystitis. In the group with calculous cholecystitis, 1 of the patients required no further treatment, 3 subsequently underwent percutaneous stone removal, 14 underwent elective cholecystectomy, 6 underwent emergency cholecystectomy and 5 died of the underlying condition shortly after cholecystostomy. In the group with acalculous cholecystitis, 12 of the patients needed no further treatment after a mean follow-up period of 12 months; 8 of these underwent follow-up ultrasound examination, which revealed gallbladder calculi in only 1 patient. Four patients underwent elective cholecystectomy, 1 underwent emergency cholecystectomy, and 4 died of the underlying condition shortly after cholecystostomy. Over the long term, 23 (79%) of the 29 patients with calculous cholecystitis underwent surgery or removal of calculi. In the other group surgery was required in only 5 (24%) of the 21 patients. The authors conclude that percutaneous cholecystostomy is a useful temporizing measure, which allows patients with calculous cholecystitis to undergo elective cholecystectomy. In most cases of acalculous cholecystitis the procedure is curative, obviating the need for cholecystectomy.

Systematic transrectal ultrasound-guided biopsy of the prostate.

This study was undertaken to confirm the utility of systematic transrectal ultrasound-guided prostate biopsy in diagnosing cancer in patients with abnormal findings on digital rectal examination or abnormal levels of prostate-specific antigen (or both). The authors also wanted to determine the diagnostic advantage of taking six sextant biopsy samples rather than four quadrant samples. In a prospective study of 669 men examined between July 1992 and April 1993 at a tertiary-care hospital, core samples were obtained from any visualized or palpated abnormalities, the three other "normal" quadrants (apices and bases) and the two parasagittal midzones. The glands of 403 of the patients (60%) had an abnormality detectable by ultrasonography, and 233 of the patients (35%) had adenocarcinoma, proven by histologic examination. Of the 169 cases of adenocarcinoma initially indicated by ultrasonography, the suspected lesion was histologically benign in 66 (39%), but malignancy was found in another portion of the gland. In 18 (8%) of the 233 patients with adenocarcinoma, the only positive result was obtained from the additional core biopsy samples from the midzone. This study confirms that the ultrasonographic characteristics of cancer are variable, that many tumours (130 [56%] in this study) are detected in areas that are normal on ultrasonography and digital rectal examination, and that the detection sensitivity is increased (by 8% in this study) when two midlobe parasagittal plane biopsy samples are added to the four standard quadrant samples.

Abdominal abnormalities in AIDS: detection at US in a large population.

PURPOSE: The authors studied the prevalence of abnormalities at abdominal ultrasonography (US) in patients with acquired immunodeficiency syndrome (AIDS), the temporal change in prevalence, and the use of US to correlate clinical indications with these abnormalities. MATERIALS AND METHODS: From 1983 to 1991, 899 AIDS patients were seen, representing 89.36% of the 1,006 AIDS patients in the province during that time. Of these, 414 underwent 684 US studies, 399 of which were able to be reviewed. RESULTS: US results showed abnormalities in 264 of the 399 studies, including splenomegaly (n = 124), lymphadenopathy (n = 83]), gallbladder and bile duct abnormalities (n = 80), hepatomegaly (n = 77), and ascites (n = 54). Clinical indications with the highest frequency of abnormal findings included hepatosplenomegaly (n = 337) and abnormal liver function tests (n = 270). Lymph nodes more than 3 cm in diameter (in 10 of 83 patients with enlarged nodes) always represented an abnormality other than reactive hyperplasia. The percentage of abnormal results went from 25% in 1984 (two of nine studies) to 81% in 1990 (77 of 95 studies). CONCLUSION: The prevalence of abdominal abnormalities depicted at US in AIDS patients has increased since 1983.

Malignant obstruction of the common bile duct: long-term results of Gianturco-Rosch metal stents used as initial treatment.

PURPOSE: To determine how long Gianturco-Rosch metal stents remain patent when used as the initial treatment for malignant obstruction of the common bile duct. MATERIALS AND METHODS: The patency of Gianturco-Rosch metal stents was prospectively studied in 26 patients with malignant obstructive jaundice. Biliary obstruction was caused by pancreatic carcinoma (n = 15), cholangiocarcinoma (n = 10), or metastatic lymphadenopathy (n = 1). Follow-up information was obtained every 3-4 months until death. RESULTS: Stent insertion was successful in all patients. Stent occlusion occurred in nine patients (35%). The overall mean patency period was 39.9 weeks. Adequate biliary drainage for a minimum of 80 weeks or until death was achieved in 19 patients (73%). Life-table analysis predicted stent patency rates of 86%, 75%, and 69% at 12, 24, and 48 weeks, respectively. CONCLUSION: These results are better than those previously reported in patients with plastic endoprostheses. The authors believe that insertion of the metal stent is the procedure of choice in patients with inoperable malignant biliary obstruction.

Accuracy of subcutaneous fat measurement: comparison of skinfold calipers, ultrasound, and computed tomography.

OBJECTIVE: The purpose of this study was to compare skinfold caliper and ultrasound measurement of subcutaneous body fat at three abdominal sites with computed tomography, which is considered to be the gold standard. DESIGN: This was a cross-sectional study in which computed-tomography, ultrasound, and skinfold caliper measurements were made at three distinct abdominal sites. All body composition and anthropometric measurements were performed on each subject on one occasion. SUBJECTS: Twenty-two subjects were recruited (13 men and 9 women). Mean ages (+/- standard deviation) were 43 +/- 4 years for the women and 51 +/- 18 years for the men. All subjects had been previously scheduled for an abdominal or pelvic computed-tomography scan at the Department of Radiology, St Paul's Hospital, Vancouver, British Columbia, Canada, and participated in the study on a volunteer basis. MAIN OUTCOME MEASURES: A better agreement was found between the skinfold calipers and computed-tomography methods than between the ultrasound and computed-tomography method for the measurement of subcutaneous body fat. This was observed when the data were analyzed for both correlational agreement and for graphical interpretation. STATISTICAL ANALYSES PERFORMED: The relationships among skinfold, ultrasound, and computed-tomography measurements were analyzed by determining Pearson correlation coefficients. A graphical method described by Bland and Altman was also used to assess agreement among the three methods. RESULTS: Significant correlation coefficients were observed between skinfold calipers and computed tomography at all three abdominal sites (site 1, r = .60, P = .003; site 2, r = .70, P = .0001; site 3, r = .73, P = .0001). Ultrasound and computed-tomography methods only showed a significant correlation at site 3 (r = .54; P = .009). The graphical method revealed that the variation in the ultrasound measurements was much greater than that of the skinfold measurements when compared to computed-tomography values. APPLICATIONS/CONCLUSIONS: The results of this study indicated that relative agreement in the measurement of subcutaneous body fat between skinfold and computed-tomography measurements was superior to that exhibited between ultrasound and computed-tomography measurements. This finding enhances the potential use of skinfold calipers in the clinical setting, particularly in view of the fact that measurement of subcutaneous body fat at different body sites is becoming increasingly important for the characterization of risk of certain disease states.

Percutaneous cholecystostomy: safety of the transperitoneal route.

PURPOSE: The authors analyzed the safety and efficacy of transperitoneal percutaneous cholecystostomy (PC) in patients with acute cholecystitis. PATIENTS AND METHODS: Thirty-four consecutive patients underwent transperitoneal PC for treatment of acute cholecystitis. A trocar technique was used for placement of the drainage catheter. RESULTS: PC was technically successful in all patients. In 27 (79%) of 34 patients, symptoms of acute cholecystitis resolved within 7 days. Minor complications occurred in two patients (5%). Both patients developed a hematoma adjacent to the gallbladder; these resolved without treatment. No cases of bile leak or bile peritonitis occurred. CONCLUSION: The transperitoneal route is safe and effective for PC.

Symptomatic hepatic cysts: percutaneous drainage and sclerosis.

PURPOSE: To evaluate the authors' experience with treatment of symptomatic hepatic cysts by means of percutaneous catheterization and sclerosis. MATERIALS AND METHODS: Twenty patients with 24 symptomatic hepatic cysts underwent percutaneous drainage and sclerosis. Ten patients had polycystic disease, and 10 had solitary cysts. Sclerosants used were alcohol, tetracycline, doxycycline, or a combination. RESULTS: Twenty-one of 24 cysts in 17 of 20 patients were treated successfully. Treatment was unsuccessful in three patients: one patient with innumerable medium-size and small cysts, one patient in whom only a needle was inserted (no catheter), and one patient with a cystic metastasis (rather than a simple cyst) that recurred. Complications included pleural effusion in two patients and secondary infection in one patient. The range of blood alcohol levels was 0-0.8 mg%. CONCLUSION: Percutaneous catheter drainage with sclerosis is an effective method of therapy for symptomatic hepatic cysts; careful patient selection is essential for proper therapy.

Quality of mucosal coating in double-contrast barium enema studies: comparison of two barium preparations.

To determine which of two types of barium preparation, a dry powder (AC-14, E-Z-EM, Montreal) or a premixed suspension (Liquid Polibar Plus, E-Z-EM), gives better mucosal coating for double-contrast enema studies, the authors compared the results of two series of 80 examinations each, one for each of the preparations. The films were retrospectively reviewed by two independent observers, who did not know which preparation had been used for a given examination. Mucosal coating was graded on a five-point scale and the amount of barium on a three-point scale. Each area of the colon--the cecum, the ascending, the transverse and the descending colon and the rectosigmoid--was rated separately. The premixed barium suspension yielded significantly better mucosal coating than the dry powder in all areas of the colon (p < 0.01). There was no significant difference between the two preparations in the amount of barium deposited in any region of the colon (p > 0.05) or in the proportion of unsatisfactory examinations (p > 0.05). The authors conclude that the premixed suspension yielded better-quality mucosal coating than a suspension made from the dry powder and resulted in a similar high proportion of technically satisfactory studies.