ABSTRACT
The present study evaluated the seminal plasma metabolome of Bos indicus Guzerá bulls with good (n = 4) and poor (n = 5) sperm freezability. Animals were raised in natural pasture of a 'Caatinga' ecosystem, in the semi-arid region of Brazil. Seminal plasma samples were subjected to gas chromatography coupled to mass spectrometry and data, analysed using bioinformatics tools (Cytoscape with the MetScape plug-in). Sixty-two metabolites were identified in the bovine seminal plasma. Fatty acids and conjugates and organic compounds were the predominant seminal fluid metabolites, followed by carboxylic acids and derivatives, amino acids, benzenes and steroids and derivatives, carbohydrates and carbohydrate conjugates and prenol lipids. Multivariate analysis indicated a distinct separation of seminal plasma metabolomes from bulls with contrasting sperm freezability. Abundances of propanoic acid, d-ribose and glycine were greater in the seminal plasma of bulls with good sperm freezability. Heptadecanoic acid and undecanoic acid were the predominant in bulls of poor sperm freezability. Propanoic acid is an energy source for spermatozoa and may act as an antimicrobial component in semen. Glycine acts against oxidizing and denaturing reactions. d-ribose is also an energy source and reduces apoptosis and oxidative stress. Undecanoic acid may protect sperm against fungal damage. This study provides fundamental information approximately the seminal plasma metabolome of tropically adapted bulls and its association with sperm freezability. However, further studies with larger groups of animals are needed to validate those metabolites as markers of sperm freezability. This strategy could support the selection of sires with superior sperm cryoresistance.
Subject(s)
Propionates , Semen , Cattle , Animals , Male , Semen/chemistry , Propionates/analysis , Propionates/metabolism , Ecosystem , Ribose/analysis , Ribose/metabolism , Spermatozoa , Phenotype , GlycineABSTRACT
É possível que a expressividade de alguns elementos do plasma seminal dos bovinos, como proteínas e hormônios, possa servir como marcadores para sêmen de alta ou baixa fertilidade. Vários estudos têm demonstrado a associação de proteínas do plasma seminal com a fertilidade de touros. Dentre as mais estudadas, destacam-se aquelas com afinidade à heparina, que exercem importantes papéis na capacitação espermática e na reação acrossômica. Alguns fatores endócrinos e/ou locais, podem estar associados à expressividade e/ou função destas proteínas, auxiliando nas condições espermáticas favoráveis à fecundação. Dentre estes, destacam-se a insulina, a leptina e o fator de crescimento semelhante à insulina do tipo I. Assim sendo, evidenciam diferenças entre animais, estando associados à estrutura e as condições metabólicas da célula espermática, auxiliando na determinação da qualidade do plasma seminal. Desta maneira, o estudo das proteínas do plasma seminal asociado à condição metabólica destes hormônios, presentes neste meio, pode servir como importante parâmetro de avaliação da condição reprodutiva do macho
It is possible that the expression of some elements in seminal plasma, such as proteins and hormones, may act as markers of quality. Several studies have demonstrated an association of seminal plasma proteins with fertility. Among the most studied, there are the proteins with affinity to heparin, which play an important role in sperm capacitation and acrosome reaction. Some endocrine factors and/or locals may be associated with the expression and function of these proteins, aiding in sperm condition conducive to fertilization. Among them stand out as insulin, leptin and growth factor insulin-like type I. Thesefactors may highlight the differences between these animals because they are associated with the metabolic condition and structure of sperm cells, aiding in determining the quality of seminal plasma. Thus, the study of seminal plasma proteins associated with the metabolic condition of these hormones are present in this medium can serve as a new parameter for assessment of male reproductive condition...
