ABSTRACT
Antler growth is highly co-ordinated, so that trabecular bone and antler skin (velvet) develop together, at a rapid rate and in a manner reminiscent of their development in the fetus. Parathyroid hormone-related peptide (PTHrP) is expressed in both bone and skin, and is therefore a candidate to effect co-ordination between these tissues. The aim of this study was to localize the expression of PTHrP and its principal receptor, the parathyroid hormone/parathyroid hormone-related peptide receptor (PTH/PTHrPR), in antler ("spiker") of one-year-old red deer. Using immunohistochemistry and in situ hybridization, intense and overlapping expression of PTHrP and its receptor was seen in developing osseocartilaginous structures and in the underlying layers of velvet epidermis. PTHrP was located on both the cell surface and within the nuclei. Our results strongly suggest that PTHrP, acting via the PTH/PTHrPR and possibly other intracrine mechanisms, plays a central role in the co-ordinated regulation of cell division and differentiation of developing antler bone and skin.
Subject(s)
Antlers/growth & development , Antlers/metabolism , Deer/growth & development , Deer/metabolism , Parathyroid Hormone-Related Protein/biosynthesis , Receptor, Parathyroid Hormone, Type 1/biosynthesis , Amino Acid Sequence , Animals , Antlers/anatomy & histology , Cloning, Molecular , Deer/anatomy & histology , Epidermis/anatomy & histology , Epidermis/growth & development , Epidermis/metabolism , Gene Expression Regulation, Developmental/genetics , Immunohistochemistry , Molecular Sequence Data , Parathyroid Hormone-Related Protein/genetics , Parathyroid Hormone-Related Protein/isolation & purification , Receptor, Parathyroid Hormone, Type 1/genetics , Receptor, Parathyroid Hormone, Type 1/isolation & purificationABSTRACT
Antlers grow rapidly through the coordinated development of both osseocartilage and skin (velvet). The regional patterns of cell division in these two compartments were assessed by immunochemical detection of proliferating cell nuclear antigen (PCNA) in antlers from one-year-old red deer. The whole antler integument was in a state of growth and/or renewal, particularly the keratinocytes of the basal cell layer of the epidermis near the tip, and hair bulbs and sebaceous glands. More proximally, a zone of weaker mitotic activity was detected. Within the osseocartilagenous compartment, rapid mitosis was particularly apparent within the distal mesenchyme, visible as a dome-shaped band of staining. Mitotic activity of chondrocytes and osteoblasts was more extensive in peripheral areas of developing bone than in the centre. We conclude that the antler tip is the site of most active epidermal growth, and hypothesise that other mechanisms in addition to mechanical stretching play a role in growth of the integument.
