ABSTRACT
13 cases of uterine carcinosarcomas have been studied by molecular-genetic method. The impairment of some genes (K-RAS gene in the first exon, TP53 in the 5th-9th exons, PTEN in the 5th and 8th exons) has been found out. The loss of heterozygosity of TP53 gene in epithelium and mesenchyme of the uterus has been detected. According to the estimation of impairment the studied tumors was divided into monoclonal and biclonal ones. This stratification could be use as prognostic criteria of uterine carcinosarcomas clinical presentation.
Subject(s)
Carcinosarcoma/genetics , Carcinosarcoma/pathology , PTEN Phosphohydrolase/genetics , Proto-Oncogene Proteins p21(ras)/genetics , Tumor Suppressor Protein p53/genetics , Uterine Neoplasms/genetics , Uterine Neoplasms/pathology , Carcinosarcoma/metabolism , Exons , Female , Humans , Loss of Heterozygosity , PTEN Phosphohydrolase/metabolism , Proto-Oncogene Proteins p21(ras)/metabolism , Retrospective Studies , Tumor Suppressor Protein p53/metabolism , Uterine Neoplasms/metabolismABSTRACT
Clinico-morphological and immunohistochemical examinations were carried out in 46 patients with carcinocarcinoma of the corpus uteri to evaluate p53, PTEN and cyclooxygenase-2 (COX-2) expression in mesenchymal and epithelial cells of tumor. p53 expression in the epithelium and that of COX-2 in both compartments appeared to be unfavorable prognosticators of survival while that of PTEN is a lesser one in significance.
Subject(s)
Biomarkers, Tumor/analysis , Carcinosarcoma/chemistry , Carcinosarcoma/pathology , Cyclooxygenase 2/analysis , PTEN Phosphohydrolase/analysis , Tumor Suppressor Protein p53/analysis , Uterine Neoplasms/chemistry , Uterine Neoplasms/pathology , Adult , Aged , Carcinosarcoma/mortality , Female , Gene Expression Regulation, Neoplastic , Humans , Immunohistochemistry , Middle Aged , Predictive Value of Tests , Prognosis , Survival Analysis , Uterine Neoplasms/mortalityABSTRACT
The data received on histological organization and cell composition of gonads in some hydrobionts complete the information about their reproductive biology and allow giving concrete expression to spawning period. The influence of some important ecological factors on reproduction of these hydrobionts has been analysed.
Subject(s)
Corbicula/physiology , Oogenesis/physiology , Spermatogenesis/physiology , Strongylocentrotus/physiology , Animals , Corbicula/ultrastructure , Female , Fisheries , Male , Seasons , Strongylocentrotus/ultrastructureABSTRACT
A morphological study of gonads in Corbicula japonica made it possible to distinguish between five stages of gonad maturity. It has been established that stages of active gametogenesis, prespawning and of spawning go almost in parallel and in the shortest time. In July, with the beginning of spawning, gametogenesis does not stop, and oogenesis proceeds up to the end of August. Dynamics of changes of some chemical substances in oocytes was followed. High concentrations of, respectively, RNA, albumens, lipids, glycoproteins and proteoglycans were revealed in oocyte nuclei and nucleoli both at the beginning of the development, and at the stage of active gametogenesis. This testifies in favor of a synthetic activity of these cells.
