Trends in Relative Age Effects of Top-Level Female Soccer Players: A Japanese Study.

We investigated recent trends in relative age effect (RAE) findings of top-level female soccer players in Japan, using data from the 2016 to 2020 seasons. We conducted two main analyses: (a) An examination of RAE for all registered players in the Japan Women's Soccer League (Nadeshiko League) from 2016 to 2020; and (b) an examination of RAE of newly registered players in the league from 2017 to 2020. In the first analysis, we found a significant difference between the number of players born in Q1 (April-June) versus Q4 (January-March), with the number of players born in Q1 greater and with the ratio between these groups ranging from 1.5 to 1.7. In the second analysis, we found a significant relationship between Q1 and Q4 for the 2017 season alone. However, the Q1/Q4 ratio ranged from 1.4 to 1.9, and the semester ratio of S1 (Q1 + Q2))/S2 (Q3 + Q4) ranged from 1.2 to 1.3, suggesting a birth month bias. Thus, there was a RAE in female soccer players playing recently in Japan's top-level leagues; and the size of the effect did not change significantly across recent seasons.

Salmonella enterica Effectors SifA, SpvB, SseF, SseJ, and SteA Contribute to Type III Secretion System 1-Independent Inflammation in a Streptomycin-Pretreated Mouse Model of Colitis.

Salmonella enterica serovar Typhimurium (S. Typhimurium) induces inflammatory changes in the ceca of streptomycin-pretreated mice. In this mouse model of colitis, the type III secretion system 1 (T3SS-1) has been shown to induce rapid inflammatory change in the cecum at early points, 10 to 24 h after infection. Five proteins, SipA, SopA, SopB, SopD, and SopE2, have been identified as effectors involved in eliciting intestinal inflammation within this time range. In contrast, a T3SS-1-deficient strain was shown to exhibit inflammatory changes in the cecum at 72 to 120 h postinfection. However, the effectors eliciting T3SS-1-independent inflammation remain to be clarified. In this study, we focused on two T3SS-2 phenotypes, macrophage proliferation and cytotoxicity, to identify the T3SS-2 effectors involved in T3SS-1-independent inflammation. We identified a mutant strain that could not induce cytotoxicity in a macrophage-like cell line and that reduced intestinal inflammation in streptomycin-pretreated mice. We also identified five T3SS-2 effectors, SifA, SpvB, SseF, SseJ, and SteA, associated with T3SS-1-independent macrophage cytotoxicity. We then constructed a strain lacking T3SS-1 and all the five T3SS-2 effectors, termed T1S5. The S. Typhimurium T1S5 strain significantly reduced cytotoxicity in macrophages in the same manner as a mutant invA spiB strain (T1T2). Finally, the T1S5 strain elicited no inflammatory changes in the ceca of streptomycin-pretreated mice. We conclude that these five T3SS-2 effectors contribute to T3SS-1-independent inflammation.

The effect of the kindergarten barefoot policy on preschool children's toes.

BACKGROUND: This study compared the effects of barefoot policy, a policy instructing preschool children to go without shoes, on untouched-toes, which do not touch the ground while standing normally, of preschool children attending kindergartens that follow this rule, to preschooler in kindergartens where they must wear shoes, i.e., no-barefoot policy. METHODS: The study used longitudinal data from measurements taken 2 years apart of the amount of times. The subjects were 59 children (34 boys and 25 girls) who went to a kindergarten that followed barefoot policy and 179 children (103 boys and 76 girls) who went to a kindergarten that did not follow barefoot policy. Images were taken of the contact surface area of the soles of the children's feet by having them stand on the measurement device with their bare feet. RESULTS: The number of untouched-toes in children participating in the study was determined from the pictures. In boys who attended kindergartens following barefoot policy, the ratio of the children without untouched-toes significantly increased for 2 years of childhood (35.3-64.7 %). The number of untouched-toes were significantly fewer in boys from kindergartens following barefoot policy than in boys from kindergartens not following the policy, and the magnitude of the difference grew for the two study years (ES: 0.41-0.63). In girls, there were no significant differences between the two groups in the ratio of girls without untouched-toes and the number of untouched-toes. CONCLUSIONS: In conclusion, the ground contact of the toes becomes better for boys in kindergarten with a barefoot policy. The results were inconclusive with regard to girls, and other factors may need to be examined. In the future, it will be necessary to increase the number of the subjects and perform detailed examinations.

Age-related, interindividual, and right/left differences in anterior-posterior foot pressure ratio in preschool children.

BACKGROUND: This study aimed to examine age-related, interindividual, and right/left differences in anterior-posterior foot pressure ratio in 764 preschool children (364 boys and 400 girls) aged 3.5-6.5 years. METHODS: Subjects maintained an upright standing posture for 10 seconds on the Footview Clinic, an instrument designed to calculate the anterior-posterior foot pressure ratio. The ratio of anterior foot pressure in each subject's right and left feet was selected as a variable, and the mean of a 10 s measurement was used for analysis. RESULTS: The ratio of anterior foot pressure was significantly larger in the right foot than in the left foot. With regard to age, the ratio of anterior foot pressure was significantly larger in children aged over 4.5 years than in children aged 3.5 years. It was also larger in children aged 6 and 6.5 years than in children aged 4 years. Interindividual differences in variables were large, and coefficients of variance were highest in children aged 3.5 years and lowest in children aged 6.5 years. CONCLUSIONS: In conclusion, anterior foot pressure increases with age in preschool children. Interindividual differences in anterior foot pressure are large and tend to decrease with age. Furthermore, the anterior foot pressure is slightly higher in the right foot than in the left foot. These results will be useful for various studies, such as examining relationships between the anterior-posterior foot pressure ratio and factors, such as untouched toes, physical fitness, and level of exercise.

Preparation and characterization of dispersible chitosan particles with borate crosslinking and their antimicrobial and antifungal activity.

We synthesized new dispersive chitosan particles at circumneutral pH. Particles composed of a chitosan-borate complex were synthesized by a method consisting of two simple steps: mixture and dialysis. As this method does not employ reagents such as organic solvents or surface-active agents and does not require heat treatment, it has a minimal negative impact on the environment. Crosslinking of the reaction of glucose and boric acid at ordinary temperature and pressure led to the formation of composite particles. Stereoscopic microscopy and investigation of the particle size distribution by dynamic light scattering (DLS) revealed that particles ranging in size from submicrons to several microns with high dispersibility in water were obtained. Even after heat treatment at 80°C for 12h, the particles maintained their composite formation, indicating that they have high thermal stability. Chitosan powders demonstrated inadequate antimicrobial properties at circumneutral pH, but the particles of the chitosan-borate complex had antimicrobial properties against the gram-negative bacterium, Escherichia coli, and the gram-positive bacterium, Staphylococcus aureus, as well as the fungi Aspergillus niger and Fusarium solani. These results indicated that the particles of the chitosan-borate complex had a broad antimicrobial spectrum at circumneutral pH.

Static one-legged balance in soccer players during use of a lifted leg.

The goal was to describe static one-legged balance during use of a lifted leg and to compare balance between the dominant and nondominant legs of soccer players. Participants were 17 male soccer players and 17 untrained male students (control). Balance ability was evaluated with four sway measures: sway velocity, anterior-posterior sway, medial-lateral sway, and high-frequency sway. Soccer players had smaller magnitude mean anterior-posterior and medial-lateral sway than untrained students. Although mean sway velocity and anterior-posterior sway were higher with the dominant leg than in the nondominant leg of the control group, there was no significant difference on any sway factor between the two legs of the soccer group. In conclusion, the soccer players were observed to have superior static one-legged balance during use of a lifted leg, and there is no difference in balance for the two legs in the soccer group.

Examining differences between center of pressure sway in one-legged and two-legged stances for soccer players and typical adults.

To examine stance during the measurement of balance ability, this study aimed to clarify the differences and relationships between balance ability in one-legged (dominant or nondominant leg) and two-legged stances in 25 typical male adults and 25 male soccer players. Balance ability was evaluated with four sway factors: sway velocity, anterior-posterior sway, lateral sway, and high-frequency sway factors. The one-legged stance was more unstable than the two-legged stance, the balance abilities related to two-legged and one-legged stances differed, and the balance abilities related to dominant-legged and nondominant-legged stances were similar. The one-legged stance, which was difficult to maintain stably, may be useful in the measurement of static balance ability in young people.

Centre of pressure sway characteristics during static one-legged stance of athletes from different sports.

The frequency of one-legged stance and two-legged stance differs considerably among sports. We therefore expect the balance ability of athletes from different sports to vary. This study compared the sway characteristics during a static one-legged stance of soccer players, basketball players, swimmers, and non-athletes. The centre of pressure sway during one-legged stance of ten male participants representing each of the four groups was measured using a stabilometer. Centre of pressure sway was assessed by four sway factors: sway velocity, anterior-posterior sway, horizontal sway, and high-frequency sway. None of the four groups of participants showed significant differences in body sway between standing on the dominant leg and standing on the non-dominant leg. The soccer players had more high-frequency sway and less anterior-posterior sway and horizontal sway than the basketball players, swimmers, and non-athletes. These results suggest that soccer players have superior ability to maintain a stable one-legged stance. Further study is required to determine how much of the superior balance ability in soccer players is innate and how much is developed through training, as well as to determine the relationship between balance ability and playing performance.