Subject(s)
Melanoma , Skin Neoplasms , Child , Fluorescence , Humans , Melanoma/diagnosis , Skin Neoplasms/diagnosis , Melanoma, Cutaneous MalignantSubject(s)
Melanoma , Mongolian Spot , Nevus, Blue , Skin Neoplasms , Adult , Dermoscopy , Diagnosis, Differential , Humans , Male , Melanoma/pathology , Nevus, Blue/diagnosis , Skin Neoplasms/diagnosis , Skin Neoplasms/pathologySubject(s)
Epstein-Barr Virus Infections/virology , Genes, T-Cell Receptor gamma/genetics , Herpesvirus 4, Human/isolation & purification , Lymphatic Diseases/virology , Lymphoma, T-Cell, Peripheral/virology , Still's Disease, Adult-Onset/virology , Epstein-Barr Virus Infections/complications , Epstein-Barr Virus Infections/genetics , Gene Rearrangement , Humans , Lymphatic Diseases/complications , Lymphatic Diseases/genetics , Lymphoma, T-Cell, Peripheral/complications , Lymphoma, T-Cell, Peripheral/genetics , Male , Middle Aged , Still's Disease, Adult-Onset/complications , Still's Disease, Adult-Onset/geneticsSubject(s)
Arthritis, Rheumatoid/drug therapy , Cell Proliferation/drug effects , Epstein-Barr Virus Infections/drug therapy , Epstein-Barr Virus Infections/pathology , Lymphomatoid Granulomatosis/drug therapy , Methotrexate/therapeutic use , Aged , Antimetabolites, Antineoplastic/pharmacology , Antimetabolites, Antineoplastic/therapeutic use , Arthritis, Rheumatoid/complications , Arthritis, Rheumatoid/virology , Epstein-Barr Virus Infections/complications , Humans , Iatrogenic Disease , Lymphomatoid Granulomatosis/complications , Lymphomatoid Granulomatosis/virology , Male , Methotrexate/pharmacologyABSTRACT
A 59-year-old asymptomatic man was incidentally found to have a periaortic mass and an elevated serum amylase level during his medical check-up. Additional findings, such as infiltration of immunoglobulin G4 (IgG4)-producing plasma cells in the mass lesion, elevation of serum IgG4 (1000 mg/dl), and pancreatic duct narrowing as evidenced on a magnetic resonance cholangiopancreatography scan, confirmed the diagnosis as retroperitoneal fibrosis complicated with autoimmune pancreatitis. The patient responded favorably to steroid treatment before the appearance of symptoms.
Subject(s)
Aortic Aneurysm, Abdominal/diagnosis , Autoimmune Diseases/diagnosis , Immunoglobulin G/immunology , Pancreatitis, Chronic/diagnosis , Retroperitoneal Fibrosis/diagnosis , Aortic Aneurysm, Abdominal/complications , Aortic Aneurysm, Abdominal/immunology , Autoimmune Diseases/complications , Autoimmune Diseases/immunology , Humans , Incidental Findings , Male , Middle Aged , Pancreatitis, Chronic/complications , Pancreatitis, Chronic/immunology , Retroperitoneal Fibrosis/complications , Retroperitoneal Fibrosis/immunologyABSTRACT
Idiopathic thrombocytopenic purpura (ITP) is a hemorrhagic disease that often occurs in the elderly. It generally presents as a chronic disease with insidious onset, and is frequently resistant to various therapies. Compared to patients less than 40 years old, those older than 60 years tend to show a higher rate of ITP-related mortality. The present report describes a 73-year-old patient with acute ITP who experienced diffuse alveolar hemorrhage that responded well to high-dose intravenous immunoglobulin therapy combined with corticosteroid pulse therapy.
Subject(s)
Hemorrhage/diagnosis , Lung Diseases/diagnosis , Pulmonary Alveoli/pathology , Purpura, Thrombocytopenic, Idiopathic/diagnosis , Acute Disease , Aged , Hemorrhage/complications , Humans , Lung Diseases/complications , Male , Purpura, Thrombocytopenic, Idiopathic/complicationsSubject(s)
Larva Migrans, Visceral/diagnosis , Toxocara canis , Adult , Animals , Ascitic Fluid , Female , Humans , Larva Migrans, Visceral/complications , Larva Migrans, Visceral/therapy , Pericardial Effusion/diagnosis , Pericardial Effusion/etiology , Pericardial Effusion/therapy , Thoracic Diseases/diagnosis , Thoracic Diseases/etiology , Thoracic Diseases/therapyABSTRACT
A CD4(+)CD8(+) double-positive thymocyte cell line, 257-20-109 was established from BALB/c mice thymocytes and used to analyze the requirements to induce CD4 or CD8 single-positive (SP) T cells. CD4SP cells were induced from 257-20-109 cells by anti-CD3 stimulation in the presence of the FcR-positive macrophage cell line, P388D1. During stimulation, maturation events, such as the down-regulation of CD24 and the up-regulation of CD69, H-2D(d), CD5, and Bcl-2, were recognized. Furthermore, these CD4SP cells appeared to be functional because the cells produced IL-2 and IL-4 when activated with phorbol ester and calcium ionophore. In contrast, CD8SP cells could be induced by stimulation with fixed anti-CD3 after removal of stimulation. To investigate the extent of signals required for CD4SP and CD8SP, the cells stimulated under either condition for 2 days were sorted and transferred to different culture conditions. These results suggested that the fate of lineage commitment was determined within 2 days, and that CD4 lineage commitment required longer activation. Furthermore, the experiments with subclones of 257-20-109 demonstrated that the lower density of CD3 did not shift the cells from CD4SP to CD8SP, but only reduced the amount of CD4SP cells. In contrast, when the 257-20-109 cells were stimulated by the combination of fixed anti-CD3 and anti-CD28, the majority of the cells shifted to CD4SP, with an enhancement of extracellular signal-regulated kinase 1 phosphorylation. Our results indicate that the signals via TCR/CD3 alone shifted the double-positive cells to CD8SP cells, but the reinforced signals via TCR/CD3 and costimulator could commit the cells to CD4SP.
Subject(s)
CD3 Complex/immunology , CD4 Antigens/analysis , CD4-Positive T-Lymphocytes/physiology , CD8 Antigens/analysis , CD8-Positive T-Lymphocytes/physiology , Cell Lineage , Animals , CD28 Antigens/physiology , CD3 Complex/analysis , Cell Line , Mice , Mice, Inbred BALB C , Mice, Inbred DBA , Mitogen-Activated Protein Kinases/physiology , Time FactorsABSTRACT
Age-related decline in thymic T-cell development in 22-month-old C57BL/6J X DBA/2J (BXD) recombinant inbred strains of mice was functionally and phenotypically analyzed and genetically mapped. There was a positive correlation of the concanavalin A (Con A)-induced thymocyte proliferative response with the capability of thymocytes to mature to the CD4(+)CD8(+) stage. The accumulation of CD4(-)CD8(-) stage of thymocytes in 22-month-old BXD mice was further identified to be associated with a developmental block between the CD25(-)CD44(+) and the CD25(+)CD44(+) stages. The quantitative trait loci regulating the Con A-induced thymocyte proliferative response were mapped to mouse chromosome 1, 3, and 11, nearest to 32.1 centimorgan (cM), 5.6 cM, and 18.0 cM, respectively. Our results suggest that several genetic loci regulate the intra-thymic T-cell maturation process and play an important role in determining age-related decline in thymic T-cell development.
