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3.
Anal Biochem ; 241(1): 47-50, 1996 Oct 01.
Article in English | MEDLINE | ID: mdl-8921164

ABSTRACT

We conducted an electron spin resonance (ESR) study on human pleural effusions obtained from patients (n = 15) with and without cancer at 77 K. We detected two kinds of signals which are considered to be due to transition metal ions in metalloproteins such as Fe3+ in transferrin and Cu2+ in ceruloplasmin from their g values (g = 4.2 and 2.049) and line shapes. We found a significant difference in the signal intensity ratio, Cu2+/Fe3+, of the pleural effusions between cancer and noncancer patients by the Mann-Whitney U test (P < 0.01). The ESR study of pleural effusion enabled us to estimate its malignancy and may have future applications.


Subject(s)
Neoplasms/diagnosis , Pleural Effusion, Malignant/diagnosis , Electron Spin Resonance Spectroscopy , Humans
5.
Intern Med ; 31(10): 1190-6, 1992 Oct.
Article in English | MEDLINE | ID: mdl-1337484

ABSTRACT

We report a rare adult case of chronic active Epstein-Barr virus (EBV) infection. A 54-year-old woman was admitted to our hospital with intermittent fever, weight loss, hepatosplenomegaly, pancytopenia and liver disturbance. In serological tests for EBV, anti-virus capsid antigen (VCA)-IgG antibody and anti-early antigen (EA)-IgG antibody were markedly elevated and anti-EBV nuclear antigen (EBNA) antibody was negative. EBV genome was detected in the bone marrow nucleated cells and peripheral lymphocytes by Southern blot hybridization. The patient developed left facial edema, bilateral breast tumor and pneumonia. She died one year after admission in spite of the administration of prednisolone, interferon and acyclovir.


Subject(s)
Capsid Proteins , Herpesviridae Infections/diagnosis , Herpesvirus 4, Human , Acyclovir/therapeutic use , Antigens, Viral/blood , Blotting, Southern , Breast Neoplasms/etiology , Chronic Disease , DNA-Binding Proteins/blood , Edema/etiology , Epstein-Barr Virus Nuclear Antigens , Female , Hepatitis/etiology , Herpesviridae Infections/complications , Herpesviridae Infections/drug therapy , Herpesviridae Infections/immunology , Herpesvirus 4, Human/genetics , Humans , Immunoglobulin A/blood , Immunoglobulin G/blood , Immunoglobulin M/blood , Interferon-alpha/therapeutic use , Liver/pathology , Middle Aged , Prednisolone/therapeutic use
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