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1.
J Oral Rehabil ; 40(5): 368-74, 2013 May.
Article in English | MEDLINE | ID: mdl-23438017

ABSTRACT

Gender-related risk factors in the survival of transplanted teeth with complete root formation have not yet been identified. The purpose of this study was to investigate gender differences in tooth autotransplantation at dental clinics. We asked participating dentists to provide information on transplantations they had undertaken from 1 January 1990 to 1931 December 2010. The data were screened to exclude patients who underwent more than one transplantation, smokers or those whose smoking habits were unknown, patients under 30 or who were 70 years old and over, cases where the transplanted teeth had incomplete root formation or multiple roots and those with fewer than 20 present teeth post-operation. We analysed 73 teeth of 73 males (mean age, 47.2 years) and 106 teeth of 106 females (mean age, 45.3 years) in this study. The cumulative survival rate and mean survival time were calculated using the Kaplan-Meier method. The cumulative survival rate for males was 88.3% at the 5-year mark, 64.8% at 10 years and 48.6% at 15 years; for females, it was 97.2% at the 5-year mark, 85.9% at 10 years and 85.9% at 15 years. A log-rank test indicated the difference between males and females to be significant (P = 0.011). There was also a significant difference in the main causes for the loss of transplanted teeth: males lost more transplanted teeth due to attachment loss than females (P < 0.05). These results indicate that males require more attention during the autotransplantation process, particularly at the stage of pre-operation evaluation and that of follow-up maintenance.


Subject(s)
Tooth Root/anatomy & histology , Tooth/transplantation , Adult , Aged , Bicuspid/pathology , Bicuspid/transplantation , Female , Follow-Up Studies , Graft Survival , Humans , Male , Middle Aged , Molar/pathology , Molar/transplantation , Odontogenesis/physiology , Periodontal Attachment Loss/complications , Prognosis , Retrospective Studies , Risk Factors , Sex Factors , Time Factors , Tooth Loss/etiology , Transplantation, Autologous , Treatment Outcome
2.
J Oral Rehabil ; 40(2): 112-8, 2013 Feb.
Article in English | MEDLINE | ID: mdl-23134328

ABSTRACT

The aim of this study was to investigate risk factors with age in the long-term prognosis of autotransplantation of teeth with complete root formation at dental clinics. Participating dentists were asked to provide information on transplantations they had undertaken from 1 January 1990 to 31 December 2010. Data on a total of 708 teeth from 637 patients were collected. The data were screened to exclude patients who were under 25 or 70 years of age and over, those who were smokers or whose smoking habits were unknown, those whose transplanted teeth had incomplete root formation or multiple roots and those with fewer than 25 present teeth post-operation. The participants in this study were 71 men (74 teeth) and 100 women (107 teeth) ranging from 25 to 69 years of age. Third molars were used as donor teeth in 89·0% of the cases. The participants were divided into three age groups of 25-39, 40-54 and 55-69. Survival analysis was conducted using the Kaplan-Meier method, and a log-rank test revealed that there were no significant differences in age groups for men or women. Cox regression analysis indicated that the survival of transplanted teeth was not influenced by age. However, although not statistically significant, the clinical success rate was lower in the 55-69-year-old group than that in the younger groups. These results indicate that if suitable donor teeth are available and the conditions are right, autotransplantation is a viable treatment for missing teeth regardless of the age of the patient.


Subject(s)
Tooth Root/growth & development , Tooth/transplantation , Adult , Age Factors , Aged , Female , Humans , Kaplan-Meier Estimate , Male , Middle Aged , Molar, Third/transplantation , Prognosis , Proportional Hazards Models , Transplantation, Autologous
3.
J Oral Rehabil ; 40(1): 33-42, 2013 Jan.
Article in English | MEDLINE | ID: mdl-22943482

ABSTRACT

The aim of this study was to compare the prognosis of separated and non-separated tooth autotransplantation of the upper first and second molars with complete root formation undertaken at dental clinics. The participating dentists were requested to provide information on transplantations they had undertaken from 1 January 1990 to 31 December 2010. Data on a total of 708 teeth from 637 patients were collected. This study analysed 35 separated teeth and 22 non-separated teeth of 47 participants ranging from 27 to 76 years of age (mean age: 55·0 years) after data screening and elimination. The cumulative post-transplantation survival rate at 10 years was 77·1% for separated teeth and 63·6% for non-separated teeth as calculated with the Kaplan-Meier method. There were no significant differences between separated teeth and non-separated teeth in a log rank test (P = 0·687). Separated-tooth autotransplantation can help fill narrow recipient sites and increase occlusal supporting zones, but the clinical success rate was only 48·6%. Although transplantation of teeth with complete root formation has limited prognosis, transplantation of upper first and second molars, whether separated or non-separated, is a viable option to replace missing teeth.


Subject(s)
Jaw, Edentulous, Partially/surgery , Molar/transplantation , Oral Surgical Procedures/methods , Tooth Root/transplantation , Adult , Aged , Female , Humans , Male , Maxilla/surgery , Middle Aged , Prognosis , Transplantation, Autologous/methods
4.
J Oral Rehabil ; 39(11): 821-9, 2012 Nov.
Article in English | MEDLINE | ID: mdl-22672336

ABSTRACT

The aim of this study was to investigate the risk factors affecting long-term prognosis of autotransplantation of third molars with complete root formation in males at dental clinics. Participating dentists were requested to provide information on transplantations they had undertaken from 1 January 1990 to 31 December 2010. Data on a total of 708 teeth from 637 patients were collected. After data screening and elimination, participants of this study consisted of 183 teeth of 171 males ranging from 20 to 72 years of age (mean age, 44·8 years). The cumulative survival rate was 86·0% at the 5-year mark, 59·1% at 10 years and 28·0% at 15 years. The mean survival time was 134·5 months, as calculated by the Kaplan-Meier method. Single factor analysis using the log-rank test showed that the following factors had significant influence (P < 0·05) on survival of transplanted teeth: periodontal disease as the reason for recipient site tooth extraction, fewer than 25 present teeth and Eichner index Groups B1 to C. Cox regression analysis examined five factors: age, smoking habit, recipient site extraction caused by periodontal disease, fewer than 25 present teeth and Eichner index. This analysis showed that two of these factors were significant: fewer than 25 present teeth was 2·63 (95% CI, 1·03-6·69) and recipient site extraction caused by periodontal disease was 3·80 (95% CI, 1·61-9·01). The results of this study suggest that long-term survival of transplanted teeth in males is influenced not only by oral bacterium but also by occlusal status.


Subject(s)
Molar, Third/transplantation , Adult , Age Factors , Aged , Crowns , Dental Abutments , Dental Caries/etiology , Follow-Up Studies , Humans , Male , Middle Aged , Periodontal Attachment Loss/etiology , Periodontitis/complications , Postoperative Complications , Retrospective Studies , Risk Factors , Root Canal Therapy , Root Resorption/etiology , Sex Factors , Smoking , Survival Analysis , Tooth Ankylosis/etiology , Tooth Extraction , Tooth Fractures/etiology , Tooth Root/injuries , Tooth Socket/surgery , Transplantation, Autologous , Treatment Outcome , Young Adult
5.
J Oral Rehabil ; 39(1): 37-43, 2012 Jan.
Article in English | MEDLINE | ID: mdl-21793869

ABSTRACT

The aim of this study was to investigate the usage of tooth autotransplantation in dental clinics which offer the treatment and evaluate its practicality. Participating dentists were requested to provide information on transplantations they had undertaken from 1 January 1990 to 31 December 2010. A total of 614 teeth from 552 patients (37 dentists) ranging in age from 17 to 79 (mean age: 44·1) were examined. Cumulative survival rate and mean survival time were calculated using the Kaplan-Meier method, and log rank test was used for analysis of factors. The mean number of autotransplantation patients per clinic per year was 1·4. Upper third molars constituted 36·8% of donor teeth, while 37·1% were lower third molars. The lower first molar region was the most common recipient site at 32·6%, followed by the lower second molar region (28·0%). Prosthodontic treatment of transplanted teeth involved coverage with a single crown (72·5%) and abutment of bridge (18·9%). A total of 102 transplanted teeth were lost owing to complications such as attachment loss (54·9%) and root resorption (25·7%). The cumulative survival rate in cases where donor teeth had complete root formation was 90·1% at 5 years, 70·5% at 10 years and 55·6% at 15 years. The mean survival time was 165·6 months. Older age was a significant risk factor (P < 0·05) for survival. In cases where suitable donor teeth are available, autotransplantation of teeth may be a plausible treatment option for dealing with missing teeth in dental clinics.


Subject(s)
Oral Surgical Procedures/statistics & numerical data , Tooth/transplantation , Adolescent , Adult , Aged , Dental Clinics , Female , Follow-Up Studies , Humans , Male , Middle Aged , Retrospective Studies , Transplantation, Autologous/statistics & numerical data , Treatment Outcome , Young Adult
6.
Int J Oral Maxillofac Surg ; 31(5): 489-94, 2002 Oct.
Article in English | MEDLINE | ID: mdl-12418563

ABSTRACT

This study examined the characteristics and outcome of patients undergoing partial inferior turbinectomy during secondary alveolar bone grafting. Thirty-three of 55 patients with cleft lip and palate or cleft lip and alveolus who underwent secondary alveolar bone grafting concurrently received partial inferior turbinectomy to ensure that the height of the nasal floor was similar on the cleft side and non-affected side. At the time of surgery, patients who underwent turbinectomy were significantly older than those who did not undergo the procedure. The proportion of patients who underwent turbinectomy was significantly higher among patients with cleft lip and palate than among those with cleft lip and alveolus. These differences apparently reflected the developmental stage of the inferior turbinate and the relative severity of alveolar and palatal defects. In most patients who underwent partial inferior turbinectomy, postoperative X-ray films revealed excellent bone formation at the graft site. Our findings suggest that partial inferior turbinectomy during secondary alveolar bone grafting is a very useful procedure that facilitates dissection to the height of the nasal floor, reconstruction of the mucosal nasal floor, and formation of a sufficient bone bridge. It also promotes alveolar cleft closure, especially in patients with wide bone defects.


Subject(s)
Alveoloplasty/methods , Bone Transplantation/methods , Cleft Lip/surgery , Cleft Palate/surgery , Turbinates/surgery , Adolescent , Adult , Age Factors , Alveolar Process/abnormalities , Bone Transplantation/diagnostic imaging , Bone Transplantation/physiology , Child , Cleft Lip/diagnostic imaging , Cleft Palate/diagnostic imaging , Female , Follow-Up Studies , Humans , Male , Nasal Cavity/diagnostic imaging , Nasal Cavity/surgery , Nasal Mucosa/surgery , Osteogenesis/physiology , Osteotomy/methods , Radiography , Statistics as Topic , Statistics, Nonparametric , Treatment Outcome
7.
Plant Physiol ; 127(4): 1626-34, 2001 Dec.
Article in English | MEDLINE | ID: mdl-11743107

ABSTRACT

Arabidopsis RD21 is a cysteine protease of the papain family. Unlike other members of the papain family, RD21 has a C-terminal extension sequence composed of two domains, a 2-kD proline-rich domain and a 10-kD domain homologous to animal epithelin/granulin family proteins. The RD21 protein was accumulated as 38- and 33-kD proteins in Arabidopsis leaves. An immunoblot showed that the 38-kD protein had the granulin domain, whereas the 33-kD protein did not. A pulse-chase experiment with Bright-Yellow 2 transformant cells expressing RD21 showed that RD21 was synthesized as a 57-kD precursor and was then slowly processed to make the 33-kD mature protein via the 38-kD intermediate. After a 12-h chase, the 38-kD intermediate was still detected in the cells. These results indicate that the N-terminal propeptide was first removed from the 57-kD precursor, and the C-terminal granulin domain was then slowly removed to yield the 33-kD mature protein. Subcellular fractionation of the Bright-Yellow 2 transformant showed that the intermediate and mature forms of RD21 were localized in the vacuoles. Under the acidic conditions of the vacuolar interior, the intermediate was found to be easily aggregated. The intermediate and the mature protein were accumulated in association with leaf senescence. Taken together, these results indicate that the intermediate of RD21 was accumulated in the vacuoles as an aggregate, and then slowly matured to make a soluble protease by removing the granulin domain during leaf senescence.


Subject(s)
Arabidopsis Proteins/metabolism , Arabidopsis/genetics , Cysteine Endopeptidases/metabolism , Intercellular Signaling Peptides and Proteins , Vacuoles/enzymology , Animals , Arabidopsis/enzymology , Arabidopsis Proteins/genetics , Chromosome Mapping , Cloning, Molecular , Cysteine Endopeptidases/genetics , Enzyme Precursors/metabolism , Expressed Sequence Tags , Maturation-Promoting Factor , Plant Leaves/enzymology , Plant Leaves/genetics , Plant Leaves/growth & development , Progranulins , Viral Proteins/genetics , Viral Proteins/metabolism
8.
Plant Cell Physiol ; 42(9): 894-9, 2001 Sep.
Article in English | MEDLINE | ID: mdl-11577182

ABSTRACT

Plants degrade cellular materials during senescence and under various stresses. We report that the precursors of two stress-inducible cysteine proteinases, RD21 and a vacuolar processing enzyme (VPE), are specifically accumulated in approximately 0.5 microm diameter x approximately 5 microm long bodies in Arabidopsis thaliana. Such bodies have previously been observed in Arabidopsis but their function was not known. They are surrounded with ribosomes and thus are assumed to be directly derived from the endoplasmic reticulum (ER). Therefore, we propose to call them the ER bodies. The ER bodies are observed specifically in the epidermal cells of healthy seedlings. These cells are easily wounded and stressed by the external environment. When the seedlings are stressed with a concentrated salt solution, leading to death of the epidermal cells, the ER bodies start to fuse with each other and with the vacuoles, thereby mediating the delivery of the precursors directly to the vacuoles. This regulated, direct pathway differs from the usual case in which proteinases are transported constitutively from the ER to the Golgi complex and then to vacuoles, with intervention of vesicle-transport machinery, such as a vacuolar-sorting receptor or a syntaxin of the SNARE family. Thus, the ER bodies appear to be a novel proteinase-storing system that assists in cell death under stressed conditions.


Subject(s)
Arabidopsis/enzymology , Cysteine Endopeptidases/metabolism , Enzyme Precursors/metabolism , Organelles/enzymology , Adaptation, Physiological , Arabidopsis/ultrastructure , Cell Death , Endoplasmic Reticulum/ultrastructure , Organelles/ultrastructure , Plant Epidermis/enzymology , Plant Epidermis/ultrastructure , Plants, Genetically Modified , Vacuoles/ultrastructure
9.
Psychol Rep ; 86(1): 333-8, 2000 Feb.
Article in English | MEDLINE | ID: mdl-10778288

ABSTRACT

The purpose of this study was to examine the effect of Shyness and Social Skills on Self-disclosure. Three scales, the Trait Shyness Scale, the Scale of Social Skill, and the Self-disclosure Questionnaire were administered to 443 university students. Scores on Shyness were highly negatively correlated with those on Social Skills and moderately negatively correlated with Self-disclosure. Furthermore, Self-disclosure scores correlated positively with Social Skills. Separate factor analyses of the Shyness and Social Skills scales yielded four and two factors, respectively. Findings suggest that shy people may have low interpersonal skills and thus, they may hesitate to engage in self-disclosure.


Subject(s)
Self Disclosure , Shyness , Social Behavior , Adolescent , Adult , Female , Humans , Interpersonal Relations , Male , Personality Inventory , Social Perception , Students/psychology
10.
Appl Opt ; 37(7): 1213-9, 1998 Mar 01.
Article in English | MEDLINE | ID: mdl-18268706

ABSTRACT

A simple fabrication technique of nonlinear optical structures for use with dye-doped polymer is described. Polymethylmethacrylate, U-100 polymer, and polystyrene were used as the host matrices to fabricate the nonlinear optical waveguide. The periodically poled nonlinear optical polymer structures and ridge-type channel structures were fabricated by direct electron-beam irradiation. The electron beam with 25 kV of energy was exposed directly onto the polymer films containing the nonlinear optical chromophores. We can also demonstrate the fabrication technique of the domain-inverted grating of dye-doped polystyrene film.

11.
Appl Microbiol Biotechnol ; 38(6): 770-5, 1993 Mar.
Article in English | MEDLINE | ID: mdl-7763534

ABSTRACT

A high-level secretion system for the production of mouse-human chimeric antibody 21B2 (MHC 21B2) Fab fragment specific for human carcino embryonic antigen (hCEA) in Escherichia coli has been constructed. The genes encoding a light chain and an Fd fragment (a variable region and the CH1 domain of a heavy chain) of a mouse-human chimeric antibody were directly fused to the signal peptide of the E. coli ompF gene sequence. E. coli cells containing expression vectors in which each of the two genes are located downstream of a separate tac promoter were able to secrete the light chain and Fd fragment as two of their major cellular proteins. The signal peptides were efficiently removed from the primary products by post-translational processing, although they formed insoluble aggregates, possibly in the periplasm. In high-cell-density culture experiments using a jar fermentor, the amount of light chain and Fd fragment produced was at levels of up to 2.88 g/l and 1.28 g/l culture, respectively. By optimizing the conditions that encourage correct folding, formation of disulphide bonds, and association of the light chain with the Fd fragment, we have established a procedure that can purify, re-fold, and combine aggregated products to electrophoretically homogeneous Fab fragment with a yield of approximately 47%. Fab fragment produced in this manner shows essentially the same antigen-binding activity and specificity to hCEA as the parental mouse antibody 21B2 (MoAb 21B2).


Subject(s)
Carcinoembryonic Antigen/immunology , Escherichia coli/genetics , Immunoglobulin Fab Fragments/biosynthesis , Amino Acid Sequence , Animals , Antibody Affinity , Antibody Specificity , Biotechnology , Genes, Immunoglobulin , Genetic Vectors , Humans , Immunoglobulin Fab Fragments/genetics , Immunoglobulin Fab Fragments/isolation & purification , Mice , Molecular Sequence Data , Recombinant Proteins/biosynthesis , Recombinant Proteins/genetics , Recombinant Proteins/isolation & purification
13.
Prev Med ; 21(4): 503-9, 1992 Jul.
Article in English | MEDLINE | ID: mdl-1409491

ABSTRACT

BACKGROUND: Our research objective is to develop nontoxic cancer chemopreventive agents and to apply these agents in treating humans. We are identifying agents that inhibit the process of tumor promotion in two-stage carcinogenesis experiments on mouse skin. METHODS: We review (a) the inhibitory effect of penta-O-galloyl-beta-D-glucose (5GG) on tumor promotion by teleocidin, one of the 12-O-tetradecanoylphorbol-13-acetate (TPA)-type tumor promoters (5GG is structurally similar to (-)-epigallocatechin gallate (EGCG) and is isolated from hydrolyzed tannic acid); (b) the inhibitory effects of EGCG, the main constituent of Japanese green tea, on tumor promotion with two tumor promoters, teleocidin and okadaic acid, a non-TPA-type tumor promoter; (c) the mechanisms of action of EGCG, a single application of which reduced the specific binding of [3H]TPA and [3H]okadaic acid to a particulate fraction of mouse skin; and (d) the anticarcinogenic effects of EGCG on duodenal carcinogenesis induced by N-ethyl-N'-nitro-N-nitrosoguanidine in male C57BL/6 mice. EGCG is a nontoxic compound. CONCLUSION: We believe that the main constituent of Japanese green tea, EGCG, is a practical cancer chemopreventive agent available in everyday life.


Subject(s)
Anticarcinogenic Agents/pharmacology , Catechin/analogs & derivatives , Hydrolyzable Tannins , Skin Neoplasms/prevention & control , Skin/drug effects , Tannins/pharmacology , Tea , Animals , Anticarcinogenic Agents/therapeutic use , Catechin/pharmacology , Catechin/therapeutic use , Male , Mice , Mice, Inbred C57BL , Skin/metabolism , Skin Neoplasms/metabolism , Tannins/therapeutic use , Tetradecanoylphorbol Acetate/analogs & derivatives , Tetradecanoylphorbol Acetate/metabolism
16.
J Cancer Res Clin Oncol ; 118(6): 420-4, 1992.
Article in English | MEDLINE | ID: mdl-1618889

ABSTRACT

Certain waterblooms of toxic cyanobacteria (blue-green algae) are a health threat because of their production of toxic peptides, termed microcystins, which cause liver damage in wild and domesticated animals. The most widely studied microcystin is microcystin-LR, a heptapeptide containing the two L-amino acids, leucine and arginine. The inhibition of protein phosphatase type 1 and type 2A activities by microcystin-LR is similar to that of the known protein phosphatase inhibitor and tumor promoter okadaic acid. We show in this report that microcystin-LR, applied below the acute toxicity level, dose-dependently increases the number and percentage area of positive foci for the placental form of glutathione S-transferase in rat liver, which was initiated with diethylnitrosamine. The result was obtained independently through two animal experiments. This observation indicates that microcystin-LR is a new liver tumor promoter mediated through inhibition of protein phosphatase type 1 and type 2A activities. This provides further evidence that the okadaic acid pathway is a general mechanism of tumor promotion in various organs, such as mouse skin, rat glandular stomach and rat liver.


Subject(s)
Liver Neoplasms/chemically induced , Marine Toxins/toxicity , Peptides, Cyclic/toxicity , Alanine Transaminase/blood , Animals , Aspartate Aminotransferases/blood , Biomarkers, Tumor/analysis , Cyanobacteria , Diethylnitrosamine/toxicity , Glutathione Transferase/analysis , Liver Neoplasms/enzymology , Liver Neoplasms/pathology , Male , Microcystins , Phenobarbital/toxicity , Rats , Rats, Inbred F344
17.
Jpn J Cancer Res ; 82(9): 993-6, 1991 Sep.
Article in English | MEDLINE | ID: mdl-1657848

ABSTRACT

Microcystins, isolated from toxic blue-green algae, are potent inhibitors of protein phosphatases 1 and 2A. Recently, we have reported that microcystin LR has a potent tumor-promoting activity on rat liver initiated with diethylnitrosamine. The structure of microcystins is unique in having an unusual amino acid, 3-amino-9-methoxy-10-phenyl-2,6,8-trimethyl-deca-4(E),6(E)-dienoic acid (Adda), which is thought to be significant for the activity. Geometrical isomers at C-7 in the Adda portion of microcystins, 6(Z)-Adda microcystins LR and RR, have been isolated from cyanobacteria. To estimate their tumor-promoting activities and to understand the importance of the Adda portion for activity, the maternal microcystins LR and RR and their isomers were subjected to examination of their interaction with protein phosphatases 1 and 2A and the release of glutamic pyruvic transaminase from rat liver. 6(Z)-Adda microcystins LR and RR bound to protein phosphatases 1 and 2A, inhibited their activities and released glutamic pyruvic transaminase from rat liver into serum, ten to one hundred times more weakly than the maternal microcystins LR and RR. These results indicated that the conjugated diene with 4(E),6(E) geometry in the Adda portion is important in the interaction with protein phosphatases.


Subject(s)
Carcinogens/metabolism , Peptides, Cyclic/metabolism , Phosphoprotein Phosphatases/metabolism , Alanine Transaminase/blood , Animals , Carcinogens/pharmacology , Cytosol/enzymology , Ethers, Cyclic/metabolism , Liver/enzymology , Liver/metabolism , Liver Neoplasms, Experimental/chemically induced , Liver Neoplasms, Experimental/metabolism , Marine Toxins , Mice , Microcystins , Okadaic Acid , Peptides, Cyclic/pharmacology , Phosphoprotein Phosphatases/antagonists & inhibitors , Phosphoprotein Phosphatases/drug effects , Protein Binding , Skin/enzymology , Stereoisomerism , Structure-Activity Relationship , Tritium
18.
Neurosci Res ; 11(2): 119-33, 1991 Jul.
Article in English | MEDLINE | ID: mdl-1717903

ABSTRACT

Distribution of axons with calcitonin gene-related peptide (CGRP)-like and/or substance P (SP)-like immunoreactivity (LI) within the sensory trigeminal nuclei was examined in the cat before and after trigeminal rhizotomy. Axons with CGRP-LI or SP-LI were seen throughout the principal sensory trigeminal nucleus (Vp) and spinal trigeminal nuclei, including the medullary dorsal horn (MDH). They were densely distributed particularly in the dorsolateral part of the dorsal subnucleus of the Vp, ventromedial marginal zone of the ventral subnucleus of the Vp, dorsomedial and ventromedial parts of the oral spinal trigeminal nucleus, ventromedial and lateral marginal zones of the interpolar spinal trigeminal nucleus, and lamina I, outer part of lamina II and lamina V of the MDH. Most of the CGRP-LI axons exhibited SP-LI, while many SP-LI axons did not show CGRP-LI. After trigeminal rhizotomy, almost all CGRP-LI axons disappeared from the ipsilateral sensory trigeminal nuclei, while a considerable number of SP-LI axons remained intact throughout the nuclei; these SP-LI axons did not show CGRP-LI. The results indicate that CGRP-LI axons within the sensory trigeminal nuclei exhibit SP-LI and are of peripheral origin, and that SP-LI axons without CGRP-LI are of central origin.


Subject(s)
Axons/chemistry , Calcitonin Gene-Related Peptide/analysis , Substance P/analysis , Trigeminal Nucleus, Spinal/chemistry , Animals , Cats , Denervation , Female , Immunohistochemistry , Male , Spinal Nerve Roots/physiology , Synapses/metabolism , Trigeminal Ganglion/chemistry , Trigeminal Nerve/physiology , Trigeminal Nucleus, Spinal/ultrastructure
19.
Jpn J Surg ; 21(2): 201-9, 1991 Mar.
Article in English | MEDLINE | ID: mdl-2051667

ABSTRACT

The removal of serum myoglobin with extracorporeal circulation using a column filled with methylmethacrylate-divinylbenzene (MMA-DVB) was studied in experimentally induced myonephropathic metabolic syndrome in the dog. All animals showed marked edema in the hind limbs and degeneration or necrosis of the adductor muscle 5 hours after the reestablishment of arterial flow. The serum levels of myoglobin, creatinine phosphokinase, glutamic oxaloacetic transaminase and aldolase increased linearly after the reperfusion of blood in the group of animals which received no extracorporeal circulation (group 1). Sediments of numerous myoglobin casts in the renal tubules and immunoreactive myoglobin in the renal epithelium of almost all the tubules were seen in 4 out of 5 cases. In the group of animals reperfused with extracorporeal circulation using the MMA-DVB column, serum myoglobin was adsorbed selectively by the column and showed a significantly lower value at 0.5, 1, 2 and 3 hours than that of the group 1 animals. Concomitantly, the immunoreactive myoglobin was absent or scant in the renal epithelium of the proximal convoluted tubule. The present study therefore indicates that extracorporeal circulation using the MMA-DVB column is useful for the removal of serum myoglobin from experimentally induced myonephropathic metabolic syndrome.


Subject(s)
Embolism/therapy , Extracorporeal Circulation , Kidney Diseases/therapy , Muscular Diseases/therapy , Myoglobin/blood , Myoglobinuria/therapy , Animals , Dogs , Female , Kidney/chemistry , Male , Muscles/chemistry , Myocardium/chemistry , Reperfusion Injury/therapy , Syndrome , Time Factors
20.
FEBS Lett ; 279(1): 115-8, 1991 Feb 11.
Article in English | MEDLINE | ID: mdl-1847341

ABSTRACT

Microcystin LR, which is a monocyclic heptapeptide containing two L-amino acids, leucine and arginine, is a new inhibitor of protein phosphatases 1 and 2A. Microcystin LR-affinity chromatography was used to purify protein phosphatase 2A as a holoenzyme. Five mg of microcystin LR were immobilized to ECH Sepharose 4B by the carbodiimide coupling reaction. Following DEAE-cellulose column chromatography, microcystin-affinity chromatography, as the second step in the procedure, resulted in purification of protein phosphatase 2A in a pure form. The enzyme isolated from mouse brain consisted of two regulatory subunits of 67 kDa and 58 kDa and a catalytic subunit of 41 kDa. Microcystin-affinity chromatography is useful for isolation of protein phosphatase 2A.


Subject(s)
Brain/enzymology , Peptides, Cyclic , Phosphoprotein Phosphatases/isolation & purification , Animals , Chromatography, Affinity , Chromatography, DEAE-Cellulose , Electrophoresis, Polyacrylamide Gel , Indicators and Reagents , Marine Toxins , Mice , Microcystins , Protein Phosphatase 2
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