ABSTRACT
The free radical theory of aging postulates that free radicals are the underlying cause of aging. One of the well-established age-associated disorders is the decrease in the insulin-stimulated glucose transport through the cell membrane which was reported to be a major mechanism underlying insulin resistance in old rats. The present study investigates the protective capacity of two known antioxidants, vitamin C and butylated hydroxytoluene (BHT), against age-associated free radical damage, which could be the reason behind the decreased insulin-stimulated glucose transport in old animals. Maximum insulin-stimulated glucose transport has been estimated in adipocytes isolated from adult 6-month-old Fischer rats and from senile 24-month-old control rats as well as from 24-month-old rats that were fed either vitamin C or BHT for 18 months. A significant increase in maximum insulin-stimulated glucose transport was observed in the BHT (p = 0.015) and the vitamin C groups (p = 0.039) compared with the control group of the same age. Significant stimulations with insulin over basal activities were also observed in the BHT group (p = 0.0001) as well as the vitamin C group (p = 0.009), while a nonsignificant stimulation (p = 0.097) was observed in the old control group. This study suggests a protective role that BHT (fat soluble) may have on the lipid moiety of the cell membrane and vitamin C (water soluble) on one or more of the intracellular cytoplasmic constituents concerned with glucose transport.
Subject(s)
Adipocytes/metabolism , Aging/metabolism , Antioxidants/pharmacology , Ascorbic Acid/pharmacology , Butylated Hydroxytoluene/pharmacology , Glucose/metabolism , Adipocytes/drug effects , Analysis of Variance , Animals , Glucose Tolerance Test , Ion Transport/drug effects , Male , Rats , Rats, Inbred F344ABSTRACT
Albino rats were treated with aqueous vitamin C solution and vitamin E solution dissolved in olive oil at two concentrations, 100 and 300 mg/kg/day, for 6 months. Some of the animals were then subjected to whole-body irradiation. Chromosomal aberrations and mitotic activity in non-irradiated and irradiated groups were recorded. Both vitamins were found to be non-mutagenic. Vitamin C exerted a radioprotective effect but vitamin E was not radioprotective and it suppressed the radioprotection otherwise produced by olive oil.
Subject(s)
Ascorbic Acid/pharmacology , Chromosome Aberrations , Radiation-Protective Agents/pharmacology , Vitamin E/toxicity , Animals , Bone Marrow/drug effects , Bone Marrow/radiation effects , Male , Mutagens/toxicity , Rats , Whole-Body IrradiationABSTRACT
Light and phase-contrast microscopic observations of excystment in Acanthamoeba castellanii have been used to classify cells in excysting populations as free trophozoites, or mature, activated, or preemergent cysts. These categories have been used to describe the kinetics of excystment. A pH of 7 and a temperature of 30 degrees C have been found to be optimal for the activation of mature cysts. Both activation and emergence are inhibited by cycloheximide and actinomycin D, but neither process is much affected by hydroxyurea. Cell-free extracts of high molecular weight components of cyst cytoplasm can support protein synthesis in vitro, although less efficiently than similar extracts from trophozoites. Evidence indicates that some of the functional RNA in the cyst extracts is synthesized before excystment.
