Effects of Benzodiazepines on Acinar and Myoepithelial Cells.

BACKGROUND: Benzodiazepines (BZDs), the most commonly prescribed psychotropic drugs with anxiolytic action, may cause hyposalivation. It has been previously shown that BZDs can cause hypertrophy and decrease the acini cell number. In this study, we investigated the effects of BZDs and pilocarpine on rat parotid glands, specifically on acinar, ductal, and myoepithelial cells. METHODS: Ninety male Wistar rats were divided into nine groups. Control groups received a saline solution for 30 days (C30) and 60 days (C60), and pilocarpine (PILO) for 60 days. Experimental groups received lorazepam (L30) and midazolam (M30) for 30 days. Another group (LS60 or MS60) received lorazepam or midazolam for 30 days, respectively, and saline for additional 30 days. Finally, other groups (LP60 or MP60) received either lorazepam or midazolam for 30 days, respectively, and pilocarpine for additional 30 days. The expression of calponin in myoepithelial cells and the proliferating cell nuclear antigen (PCNA) in acinar and ductal cells were evaluated. RESULTS: Animals treated with lorazepam showed an increase in the number of positive staining cells for calponin as compared to control animals (p < 0.05). Midazolam administered with pilocarpine (MP60) induced an increase in the proliferation of acinar and ductal cells and a decrease in the positive staining cells for calponin as compared to midazolam administered with saline (MS60). CONCLUSION: We found that myoepithelial cells might be more sensitive to the effects of BZD than acinar and ductal cells in rat parotid glands.

Evaluation of prevalence in the treatment of mandible condyle fractures.

OBJECTIVE: To study the treatment of fractures of the mandibular condyle and discuss conservative versus surgical therapy. METHODS: We examined the medical records of 892 bucofacial traumas, from which we selected only those who had: reports of condylar fractures, isolated or associated with other facial bones, identification data, dental care history and treatment applied for the condylar fracture. Data were analyzed using descriptive statistics, and the conservative and surgical therapies were compared. RESULTS: Condyle fractures were present in 124 cases. Males represented 72.0% of the sample, the age group most affected being the one between 21 and 30 years. Conservative treatment was used in 61.0% of patients. CONCLUSION: Surgical treatment was predominantly used in patients over ten years old, victims of traffic accidents and falls, followed by assaults, firearms and sporting accidents.

Avaliação da prevalência do tratamento das fraturas de côndilo mandibular / Evaluation of prevalence in the treatment of mandible condyle fractures

OBJETIVO: Realizar um estudo do tratamento das fraturas do côndilo mandibular e discutir a terapêutica conservadora versus a cirúrgica. MÉTODOS: Foram examinados 892 prontuários de traumatismo bucofacial, e selecionados aqueles em que haviam: relatos de fraturas condilares isoladas ou associadas a outros ossos da face, dados relativos à identificação, a história médico-odontológica, e o tratamento para a fratura de côndilo. Os dados foram analisados através de estatística descritiva e comparados a~ terapêuticas conservadora e cirúrgica. RESULTADOS: As fraturas de côndilo perfizeram um total de 124 casos. O sexo masculino representou 72,0% da amostra, e a faixa etária mais acometida foi aquela dos 21 a 30 anos. O tratamento conservador foi empregado em 61,0% dos pacientes. CONCLUSÃO: O tratamento cirúrgico foi utilizado em pacientes acima de dez anos de idade, vítimas de acidentes de trânsito e quedas, predominantemente, seguido de agressões, armas de fogo e acidente esportivo.

OBJECTIVE: To study the treatment of fractures of the mandibular condyle and discuss conservative versus surgical therapy. METHODS: We examined the medical records of 892 bucofacial traumas, from which we selected only those who had: reports of condylar fractures, isolated or associated with other facial bones, identification data, dental care history and treatment applied for the condylar fracture. Data were analyzed using descriptive statistics, and the conservative and surgical therapies were compared. RESULTS: Condyle fractures were present in 124 cases. Males represented 72.0% of the sample, the age group most affected being the one between 21 and 30 years. Conservative treatment was used in 61.0% of patients. CONCLUSION: Surgical treatment was predominantly used in patients over ten years old, victims of traffic accidents and falls, followed by assaults, firearms and sporting accidents.

The effects of antidepressants and pilocarpine on rat parotid glands: an immunohistochemical study.

OBJECTIVES: To evaluate the effects of antidepressants and pilocarpine on the quantity of myoepithelial cells and on the proliferation index of the epithelial cells of rat parotid glands. INTRODUCTION: Hyposalivation, xerostomia, and alterations in saliva composition are important clinical side effects related to the use of antidepressants. METHODS: Ninety male Wistar rats were allocated to nine groups. The control groups received saline for 30 (group C30) or 60 days (group C60) or pilocarpine for 60 days (group Pilo). The experimental groups were administered fluoxetine (group F30) or venlafaxine for 30 days (group V30); fluoxetine (group FS60) or venlafaxine (group VS60) with saline for 60 days; or fluoxetine (group FP60) or venlafaxine (group VP60) with pilocarpine for 60 days. Parotid gland specimens were processed, and the immunohistochemical expression of calponin and proliferating cell nuclear anti-antigen on the myoepithelial and parenchymal cells, respectively, was evaluated. Analysis of variance (ANOVA), Tukey HSD and Games-Howell tests were applied to detect differences among groups (p<0.05). RESULTS: Compared with the controls, chronic exposure to antidepressants was associated with an increase in the number of positively stained cells for calponin. In addition, venlafaxine administration for 30 days was associated with an increase in the number of positively stained cells for proliferating cell nuclear anti-antigen. Fluoxetine and pilocarpine (group FP60) induced a significant decrease in the number of positively stained cells for calponin compared with all other groups. CONCLUSIONS: The number of positively stained cells for calponin increased after chronic administration of antidepressants. The proliferation index of the epithelial cells of rat parotid glands was not altered by the use of antidepressants for 60 days.

The effects of antidepressants and pilocarpine on rat parotid glands: an immunohistochemical study

OBJECTIVES: To evaluate the effects of antidepressants and pilocarpine on the quantity of myoepithelial cells and on the proliferation index of the epithelial cells of rat parotid glands. INTRODUCTION: Hyposalivation, xerostomia, and alterations in saliva composition are important clinical side effects related to the use of antidepressants. METHODS: Ninety male Wistar rats were allocated to nine groups. The control groups received saline for 30 (group C30) or 60 days (group C60) or pilocarpine for 60 days (group Pilo). The experimental groups were administered fluoxetine (group F30) or venlafaxine for 30 days (group V30); fluoxetine (group FS60) or venlafaxine (group VS60) with saline for 60 days; or fluoxetine (group FP60) or venlafaxine (group VP60) with pilocarpine for 60 days. Parotid gland specimens were processed, and the immunohistochemical expression of calponin and proliferating cell nuclear anti-antigen on the myoepithelial and parenchymal cells, respectively, was evaluated. Analysis of variance (ANOVA), Tukey HSD and Games-Howell tests were applied to detect differences among groups (p<0.05). RESULTS: Compared with the controls, chronic exposure to antidepressants was associated with an increase in the number of positively stained cells for calponin. In addition, venlafaxine administration for 30 days was associated with an increase in the number of positively stained cells for proliferating cell nuclear anti-antigen. Fluoxetine and pilocarpine (group FP60) induced a significant decrease in the number of positively stained cells for calponin compared with all other groups. CONCLUSIONS: The number of positively stained cells for calponin increased after chronic administration of antidepressants. The proliferation index of the epithelial cells of rat parotid glands was not altered by the use of antidepressants for 60 days.

Salivary flow rate, calcium, urea, total protein, and amylase levels in fanconi anemia.

OBJECTIVES: Fanconi anemia (FA) is a genetic disease characterized by a chromosomal instability that develops a progressive pancitopenia, leukemia, and/or solid tumors. Nevertheless, it is unknown if this illness induces changes on the salivary gland parenchyma and function. The aim of this study was to assess the stimulated salivary flow rate (SSFR) and calcium, urea, total protein, and amylase levels in saliva of FA patients. METHODS: Stimulated whole saliva was collected from 34 randomly selected FA patients and 34 age-matched and sex-matched controls. Both samples were analyzed for salivary flow rate, calcium, urea, total proteins, and amylase. The SSFR was analyzed by gravimetric method and calcium, urea, total protein, and amylase concentrations were realized by chemistry tests. RESULTS: Mean values of SSFR for experimental and control groups were, respectively, 0.5 mL/min and 0.8 mL/min (P<0.05). Calcium concentration was 36% (P<0.05) and urea concentration was 21% (P<0.01) lower in the FA group saliva compared with saliva from the controls. The saliva concentration of amylase was almost equal in both groups. CONCLUSIONS: FA patients may exhibit significant changes in SSFR, calcium, and urea concentration of saliva.

Estudo epidemiológico das fraturas do côndilo da mandíbula / Epidemiological study of mandible condyle fractures

Introdução: Estudos epidemiológicos possibilitam analisar as particularidades regionais a respeito da etiologia dos traumas maxilo-faciais, a frequência, distribuição e determinantes dos eventos relacionados à traumatologia. Objetivo: Realizar estudo retrospectivo das fraturas do côndilo mandibular tratadas no Hospital Santa Marcelina, São Paulo/SP, atendidos no período de janeiro de 2000 a dezembro de 2007. Métodos: Foram examinados 892 prontuários e coletados dados relativos aos pacientes. Etiologia dos traumatismos e localização anatômica das fraturas foram analisadas. Resultados: As fraturas de côndilo perfizeram um total de 124 casos. O gênero masculino representou 72% da amostra, sendo a faixa etária mais acometida entre 21 a 30 anos. As causas mais frequentes foram acidentes autoviários (55,9%). Conclusões: Pacientes masculinos, na 3ª década de vida, são os mais acometidos no traumatismo bucomaxilofacial, sendo acidentes autoviários as principais causas.

Introduction: Epidemiological studies enable the analysis of the regional oddities in point of maxillofacial traumas etiology, the frequency, arrangement and determining of the events related to traumatology. Objetive: To study retrospectively the mandibular condyle fractures treated at Santa Marcelina Hospital, São Paulo/SP from January, 2000 to December, 2007. Methods: 892 records were evaluated and the data related to the patients were collected. The etiology of the traumas and the anatomic location of the fractures were analyzed. Results: The total condyle fractures were an amount of 124 cases. The male gender represented 72% of the sample and the most affected age group was 20 to 30 years. The most frequent cause was vehicular accident (55.9%). Conclusions: The conclusion is that male patients, on the third decade of life, are the most affected of maxillofacial trauma, being the vehicular accidents the main cause.

Oral focal mucinosis.

Oral focal mucinosis is an uncommon clinicopathologic condition that is considered the oral counterpart of cutaneous focal mucinosis. It is a disease of unknown etiology where the connective tissue undergoes a focal myxoid degeneration. A literature review disclosed 47 published cases of oral focal mucinosis. An additional case is presented; the clinical and histologic differential diagnosis, sex, age, location, treatment, and recurrence are discussed.

Concentração de flúor em bebidas das escolas de Curitiba / Fluor concentration in drinks of the Curitiba´s school

Foi realizada pesquisa em uma distibuidora de bebidas que fornece refrescos não alcoólicos para 05 escolas públicas municipais e 04 escolas particulares do primeiro e segundo grau na cidade de Curitiba-PR, Brasil. O estudo objetivou descobrir quais os refrescos mais consumidos por esta amostra de adolescentes e posteriormente foi analisada a concentração de flúor pelo método colorimétrico SPANDS e o potencial hidrogeniônico (pH) pelo método eletrométrico destas bebidas

Comparative study of chronological, bone, and dental age in Fanconi's anemia.

Fanconi's anemia (FA) is a genetic autosomal recessive disorder, characterized by progressive bone marrow failure, congenital abnormalities, growth retardation, and predisposition to leukemia and solid tumors. The purpose of this study was to compare the chronological age (CA), bone age (BA), and dental age (DA) of FA patients and to determine whether the therapy received influenced the BA or DA. Thirty FA patients were evaluated and divided into three groups according to the therapy received. Hand and wrist radiographs for BA estimation and panoramic radiographs for DA estimation were taken. Statistical analysis demonstrated that mean values for CA, BA, and DA were 8.91 years old, 7.90 years old, and 7.75 years old, respectively, indicating low mean values for BA and DA in comparison with CA (P<0.01). The therapy given to the patient did not influence the BA or DA. BA and DA are delayed and are not influenced by the therapy given.

Reações adversas medicamentosas de interesse odontológico / Adverse drug effects of dental interest

Diversas drogas podem estar associadas com alterações patológicas nos tecidosbucais. As apresentações clínicas são heterogêneas e podem exacerbar-se quando há sinergismo entre os fármacos. As reações adversas às drogas dependem do fármaco, da farmacodinâmica, da farmacocinética e da variabilidade individual. As alterações são bastante variáveis, incluindo desde vermelhidão ou eritema na pele até anafilaxia. O artigo faz uma breve revisão da literatura destacando algumas manifestações clínicas bucais provocadas por alguns grupos de drogas