[Usefulness of selected circulating biochemical markers in diagnosis and monitoring of endometriosis]. / Przydatnosc wybranych, krazacych markerów biochemicznych w diagnostyce i monitorowaniu endometriozy.

A review of the literature for searching of biochemical marker of endometriosis is presented. The investigations with CA 125, antiendometrial antibodies, placental protein 14 and others have not established a sensitive screening test for predicting endometriosis, especially in early stages of the disease. Most authors confirm usefulness of those markers in monitoring therapy and predicting recurrences. This may allow to avoid commonly performed "second look" laparoscopy.

[The "Bizzare" leiomyoma in histopathological examination of the uterine myoma: dilemma of the range of operative treatment]. / "Bizzare" leiomyoma w badaniu histopatologicznym miesniaka trzonu macicy--dylemat zakresu leczenia operacyjnego.

The very rare case of leiomyoma sarcomatosum was presented and differentiated to "bizzare" leiomyoma at 39 years old woman, who was conservative preoperation diagnosis of uterine myoma. To get the after-operation prognosis the following elements of tumour have been studied: mitotic index, presence and type of necrosis, separation of the tumour and perivascular infiltration. A decision of conservative treatment was confirmed by shown world wide literature.

Determination of allantoin in biological, cosmetic, and pharmaceutical samples.

Methods for determining allantoin in biological, cosmetic, and pharmaceutical samples are reviewed. The methods cover 3 main categories: spectrophotometry, alkalimetric titration, and chromatography (thin-layer and liquid). Comments are given on suitability for different sample types and on further development of methods for allantoin determination.

[Cholesterol and its fractions in women after ovariectomy treated with estraderm TTS]. / Cholesterol i jego frakcje u kobiet po wycieciu jajników leczonych Estradermem TTS.

25 ovariectomized women mean age 47 +/- 5 years were treated with transdermal 17 beta Estradiol in the shape of Estraderm TTS 100 plasters. Drugs were administrated in 28 days cycles during 24 weeks. Chlormadinon in daily dose 2 mg was added from 16 to 28th day of the fourth therapeutic cycle. Total cholesterol, triglycerides, HDL and LDL fractions were checked using enzymatic method. First measurement was done before the treatment, the next in the last day of every cycle. Results were statistically analysed. After treatment there were significant increase in the mean values of HDL, significant decrease of triglycerides. Changes in mean level of LDL and total cholesterol were not statistically significant. Our results seem to confirm the previous observations concerned with transdermal estrogen replacement therapy and its efficacy in atherosclerosis prophylaxis.

Modification of nonionic adsorbent with eriochrome blue-black R for selective nickel(II) preconcentration in conventional and flow-injection atomic-absorption spectrometry.

Chemical modification of nonionic sorbent Amberlite XAD-2 or anion exchanger Amberlyst A-26 with Eriochrome Blue-Black R (EBBR) produces a chelating resin of satisfactory chemical stability and resistance towards mineral acids. Retention of 10 metal ions has been examined for both resins. EBBR loaded XAD-2 was utilized for nickel(II) preconcentration in atomic-absorption spectrometry. In optimal conditions at a preconcentration time not exceeding 1 hr, nickel(II) can be determined at the 0.1 mug/l. level in flow measurements. Retention of metal ions on chelating resin is a convenient method of preconcentration and elimination of matrix interferences.

Operation of ion-selective electrode detectors in the sub-Nernstian/linear response range: application to flow-injection/enzymatic determination of L-glutamine in bioreactor media.

A novel approach for eliminating positive errors from endogenous ionic interferences when using ion-selective electrodes as detectors in flow-injection enzyme-based blosensing configurations is described. The method involves using a high background level of interfering ions in the sample diluent/carrier stream to convert the normally logarithmic potentiometric sensor into a linear detector over a given concentration range of primary ions. A split-stream single-detector arrangement provides a convenient means to compensate for varying levels of background interferent ions in the injected samples. One portion of the split stream passes directly to the ion-electrode detector, yielding a signal linearly related to the concentration of endogenous primary ions in the sample. The second portion of the split sample is delayed while passing through an immobilized enzyme that generates electrode detectable primary ions in proportion to the concentration of the substrate analyte in the sample. Two linear equations with two unknowns describe the twin potentiometric responses observed. The concept is demonstrated by the accurate determination of L-glutamine in hybridoma bioreactor media via the use of an ammonium-ion-selective membrane electrode detector and immobilized glutaminase enzyme.

Enzyme entrapped polypyrrole modified electrode for flow-injection determination of glucose.

Immobilization of glucose oxidase in electropolymerized polypyrrole film on the surface of a platinum wire electrode, provides a convenient sensor for flow-injection glucose determination. An upper limit of linear response for 100 microliters injected sample volume was estimated as 20 mM, whereas a 500 microliters injected sample volume gave an estimated detection limit of 0.5 mM. A simple electrode preparation procedure allows quick electrode renewal before each series of measurements.

Potentiometric stripping determination of nickel at a dimethylglyoxime-containing graphite paste electrode.

Potentiometric stripping determination of nickel(II) can be performed at zero current with a graphite paste electrode chemically modified with dimethylglyoxime. Among oxidants investigated, atmospheric oxygen present in solution was selected as the best, providing sharp and reproducible analytical signals.

[Hysterectomy within the scope of cesarean section]. / Hysterektomie im Rahmen der Sectio caesarea.

In this paper 19 cases of caesarean hysterectomy were analysed with regard to indications, choosing the method of operation, post-surgery procedure and management in cases of surgical complications. In period 1975-1985 in Ist Clinic of Obstetrics and Gynecology in Bytom we had 2,961 sections. Caesarean hysterectomy occurred in 0.07% of normal deliveries and in 0.64% of caesarean sections. The most frequent indication for caesarean section followed hysterectomy was placenta praevia (28%) and the most frequent indication for hysterectomy was atony and coagulopathy (32%). In all cases a total abdominal hysterectomy has been made. Mortality rate was 10.5% and the most frequent complications were lesions of urinary bladder (10.5%). The results obtained have been compared with the data from world literature.

A potassium-selective electrode with solid internal contact.

The performance characteristics of potassium-selective electrodes made with valinomycin membranes plasticized with dioctyl adipate or sebacate, and with a solid silver contact or an internal solution, have been critically examined. The choice of electrode depends on a number of factors, including the interfering ion(s) present. The electrodes can be used for determination of potassium in natural waters.