[Life quality in patients after endoscopic microdiskectomy for intervertebral hernia of the lumbar spine].

A group of 38 patients with hernia of the lumbar intravertebral disks who had undergone endoscopic microdiskectomy by DESTANDO was evaluated for life quality and compared with the control group (the patients had been operated on by the routine procedure and healthy individuals). The incidence of the postdiskectomic syndrome was studied in the compared groups of patients. It was shown that life quality in patients with lumbar pains could be evaluated, by using the general health status questionnaire and the specific lumbar pain questionnaire MiGAN. The life quality during the follow-up was considerably higher than that after endoscopic microdiskectomy, in males in particular. The use of endoscopic microdiskectomy reduced the incidence of the postdiskectomic syndrome by more than twice.

[The use of ultraviolet-irradiated plasma in the treatment of peritonitis]. / Primenenie UF-obluchennoi plazmy v lechenii peritonita.

Intravenous infusion of the one-group lyophilized donor plasma irradiated with ultraviolet rays was included in the complex treatment of 43 patients with acute purulent peritonitis. The species-specific composition of the microflora was studied in the different groups of patients according to the cause of peritonitis and the degree of microbial contamination of the purulent wounds in patients treated with and without ultraviolet plasma irradiation (UVPI). For prognosticating the course and outcome of the disease the authors used a complex of indices linked with the manifestation of neutrophil bactericidal activity and the level of exogenous intoxication. A positive effect of UVPI in the management of acute purulent appendicitis was noted, which was confirmed by clinical and microbiological tests.

[Possible approaches to an analysis of the heterogeneous response of the transcription complex to cholinergic actions]. / O vozmozhnykh podkhodakh k analizu geterogennoi otveta transkriptsionnogo kompleksa na kholinergicheskie vozdeistviia.

The accumulation of tyrosine-aminotransferase (TAT) as a marker of the individual gene activation was studied in the rat tissue after the administration of cholinomimetics and cholinolytics in order to elucidate the relations between cholinoreceptor functional state and the genetic apparatus. The regulation of TAT synthesis was found to be controlled by both cholinomimetic concentration and the density of cholinoreceptors in hepatocytes. Transsynaptic regulation of TAT activity was shown to be different in the brain and liver. It is suggested that the approaches discussed might be useful for the analysis of the relationship between cholinoreceptor state and the regulation of biochemical functions of target cells.

[Transcriptional complex of hepatocytes during cholinergic stimulation]. / Issledovanie transkriptsionnogo kompleksa gepatotsitov pri kholinergicheskoi stimuliatsii.

Injections of carbamyl choline into rats stimulated RNA synthesis in liver tissue involving initially the ribosomal RNA. The experiments with chromatin isolated from hepatocytes showed that carbamyl choline induced the matrix activation. During incubation of the isolated nuclei in the medium of high ionic strength (0.4 M (NH4)2SO4) containing alpha-amanitine and the enzyme components of the transcriptional complex, the RNA polymerase I was only activated. An increase in the parasympathetic innervation tonus, simulated by cholinomimetics, was accompanied by transcriptional activation with simultaneous derepression of a certain part of genome, including the genes responsible for synthesis of DNA-dependent RNA polymerases.

[Relation of the cyclic nucleotide system with the functional state of the cholinoreceptors]. / Sviaz' sistemy tsiklicheskikh nukleotidov s funktsional'nym sostoianiem kholinoretseptorov.

It has been demonstrated in rat experiments that the concentration of cAMP and cGMP in the rat liver increased 15-90 min after administration of the cholinomimetic carbamylcholine (Cr) or the cholinolytic chlorosyl (Ch) at doses of 1 and 2 mg/kg, respectively. Administration of Ch 5 min before Cr blocked the signs of parasympathetic nervous system excitation and made the content of cGMP return to normal. The concentration of cAMP in the liver of these rats as was the case in administration of Ch alone was greatly enhanced, reaching 400% of the control after 60 min. Transcriptional activity of hepatocytes following separate or combined administration of Ch and Cr was increased in 6 hours. Preliminary administration of Ch at a dose of 0.025 mg/kg prevented the excitation of the parasympathetic nervous system and stimulation of hepatocyte transcriptional activity, bringing about a dramatic fall in the liver cAMP and cGMP content. The existence of the relationship between cholinoreceptor function and the pool of cyclic nucleotides in the target cells is postulated.

[Use of electrical muscle stimulation for the prevention of neuormuscular disorders during 45 days' antiorthostatic hypokinesia]. / Primenenie élektrostimuliatsii myshts dlia profilaktiki neiromyshechnykh rasstroistv pri 45-sutochnoi antiortostaticheskoi gipokinezii.

Healthy test subjects exposed to a 45-day head-down tilting (- 6.5 degrees) showed a decline of the tone and strength of certain muscle groups, a decrease of leg circumference, a deterioration of the walking pattern, and a reduction of exercise tolerance (provocative tests with bicycle ergometry pedaling at a moderate and maximum rate). Electrostimulation of muscles applied by the Tonus-2 equipment for 30 min twice a day, 6 days a week helped to reduce the level of hypokinesia-induced disorders.