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Vestn Ross Akad Med Nauk ; (3): 10-4, 2011.
Article in Russian | MEDLINE | ID: mdl-21542369

ABSTRACT

The paper presents the results of research on the development of effective solid-phase ELISA methods for the identification of avian influenza virus (AIV) and typing its H5 and N3 subtypes in different biological samples. Optimized schemes for the production of goat antisera to matrix protein, hemagglutinin H5 and neuraminidase N3 of AIV were proposed. They were used to produce active and specific immunoglobulins and conjugates. Conditions of performing solid-phase ELISA with use of these preparations were optimized.


Subject(s)
Enzyme-Linked Immunosorbent Assay , Influenza A Virus, H5N1 Subtype/isolation & purification , Influenza in Birds/diagnosis , Animals , Birds , Goats , Hemagglutinin Glycoproteins, Influenza Virus/immunology , Influenza A Virus, H5N1 Subtype/classification , Influenza in Birds/virology , Neuraminidase/immunology , Viral Matrix Proteins/immunology
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