[The chronobiological aspects of lysosomal enzyme activity in the synovial fluid and of remission in rheumatoid inflammation]. / Khronobiologicheskie aspekty aktivnosti lizosomnykh fermentov sinovial'noi zhidkosti i remissii revmatoidnogo vospaleniia.

A study of activity of 4 lysosomal enzymes (LE) (deoxyribonuclease, acid phosphatase, acid cathepcines and beta-galactosidase) in synovial fluid (SF) depending on month of obtaining of the sample was conducted in 112 patients with rheumatoid arthritis (RA). A statistically significant chronobiological difference in this parameter was shown for all investigated enzymes with its increasing in spring and autumn and decreasing in summer and winter. The mean duration of the disease was 9.05 +/- 0.51 years. The dependence of duration of the effect after single intraarticular injection of glucocorticosteroids (methylprednisolone acetate, triamcinolone acetonide, the composition of the latest with cyclophosphamide and dymethylsulfoxide) upon the month of the puncture was investigated in 194 patients with RA. A statistically significant fluctuation (r < 0.05) of remission duration after single injection was shown only for triamcinolone acetonide +/- cyclophosphamide, but the analysis of graphic distribution gives an opportunity to imagine such a chronobiological law for other lysosomal enzymes. The identical distribution of extremes of LE activity and duration of effect after single intraarticular injection of glucocorticosteroids suggests the chronobiological link of these two parameters.