Prevalence of different Rickettsia spp. in Ixodes ricinus and Dermacentor reticulatus ticks (Acari: Ixodidae) in north-eastern Poland.

In two surveys conducted in April and May 2013, a total of 1148 unfed ticks belonging to two species, Ixodes ricinus and Dermacentor reticulatus, were collected by flagging lower vegetation in 15 different localities throughout the Bialowieza Primeval Forest (Podlaskie voivodship; north-eastern Poland) and in its buffer zone. In order to establish their infection rate with Rickettsia spp. individual adult ticks and pooled nymphs were tested by real-time PCR targeting the gltA gene. For the further identification of rickettsial species, positive samples were subjected for nested and semi-nested PCR targeting ompA and 16S rRNA genes, respectively, followed by sequencing analysis. Rickettsial DNA was detected in at least 279 ticks (minimum infection rate [MIR], 23.9%), including 52 nymphal and adult I. ricinus (MIR 8.6%) and 222 adult D. reticulatus (41%). Three species of SFG rickettsiae were identified: Rickettsia helvetica and 'Candidatus R. mendelii' in I. ricinus and R. raoultii in D. reticulatus and I. ricinus. Moreover, unidentified Rickettsia spp. which showed 99.4% identity, among others, with the uncultured Rickettsia sp. isolated from Cicadella viridis leafhopper, Rickettsia endosymbiont of Lasioglossum semilucens bee and R. bellii, were detected in I. ricinus, while Rickettsia sp. 98.3-98.4% homologous to Rickettsia secondary endosymbionts of Curculio spp. weevils was found in D. reticulatus. These results confirm the diversity of rickettsiae occurring in Poland. Further studies are needed to expand the knowledge on the species spectrum, prevalence and epidemiology of SFG rickettsiae in the country.

Kampinos National Park: a risk area for spotted fever group rickettsioses, central Poland?

Ixodid ticks are important vectors of a variety of bacterial and protozoan pathogens which cause infections in humans. In this study, altogether 1041 questing Ixodes ricinus (n = 305) and Dermacentor reticulatus ticks (n = 736), sympatrically occurring in Kampinos National Park (KPN), central-east Poland, were analyzed by PCR for Rickettsia species. Overall, the pathogen prevalence in ticks was 27.5 % for I. ricinus and 42.8 % for D. reticulatus. Sequencing analysis showed that the first tick species was exclusively infected with R. helvetica, whereas the latter was infected with R. raoultii. These organism may pose a threat for populations exposed to ticks. Preliminary results of a serosurvey of 74 KPN employees, inhabitants and visitors from the same area showed a 31.1 % total seroprevalence against SFG rickettsiae compared to 13.3 % seropositive blood donors of the control group. Risk factors significantly associated with IgG seropositivity were: occupational exposure to ticks (p = 0.002), frequency of tick bites (p = 0.02) and male gender (p = 0.005). Seropositive and seronegative individuals occupationally exposed to ticks did not differ significantly with respect to age and years of employment.