ABSTRACT
The glmS ribozyme is a bacterial gene-regulating riboswitch that controls cell wall synthesis, depending on glucosamine-6-phosphate as a cofactor. Due to the presence of this ribozyme in several human pathogen bacteria (e.g., MRSA, VRSA), the glmS ribozyme represents an attractive target for the development of artificial cofactors. The substitution of the ring oxygen in carbohydrates by functionalized methylene groups leads to a new generation of glycomimetics that exploits distinct interaction possibilities with their target structure in biological systems. Herein, we describe the synthesis of mono-fluoro-modified carba variants of α-d-glucosamine and ß-l-idosamine. (5aR)-Fluoro-carba-α-d-glucosamine-6-phosphate is a synthetic mimic of the natural ligand of the glmS ribozyme and is capable of effectively addressing its unique self-cleavage mechanism. However, in contrast to what was expected, the activity is significantly decreased compared to its non-fluorinated analog. By combining self-cleavage assays with the Bacillus subtilis and Staphylococcus aureus glmS ribozyme and molecular docking studies, we provide a structure-activity relationship for fluorinated carba-sugars.
Subject(s)
Bacterial Proteins/metabolism , Carbasugars/metabolism , RNA, Catalytic/metabolism , Bacillus subtilis/metabolism , Bacterial Proteins/chemistry , Binding Sites , Carbasugars/chemical synthesis , Carbasugars/chemistry , Cyclohexanols/chemical synthesis , Cyclohexanols/chemistry , Cyclohexanols/metabolism , Cyclohexylamines/chemical synthesis , Cyclohexylamines/chemistry , Cyclohexylamines/metabolism , Halogenation , Molecular Conformation , Molecular Docking Simulation , Nucleic Acid Conformation , RNA, Catalytic/chemistry , Staphylococcus aureus/metabolism , Stereoisomerism , Structure-Activity RelationshipABSTRACT
The ever-growing number of pathogenic bacteria resistant to treatment with antibiotics call for the development of novel compounds with as-yet unexplored modes of action. Here, we demonstrate the inâ vivo antibacterial activity of carba-α-d-glucosamine (CGlcN). In this mode of action study, we provide evidence that CGlcN-mediated growth inhibition is due to glmS ribozyme activation, and we demonstrate that CGlcN hijacks an endogenous activation pathway, hence utilizing a prodrug mechanism. This is the first report describing antibacterial activity mediated by activating the self-cleaving properties of a ribozyme. Our results open the path towards a compound class with an entirely novel and distinct molecular mechanism.
Subject(s)
Anti-Bacterial Agents/pharmacology , Bacillus subtilis/drug effects , Cyclohexanols/chemistry , Cyclohexylamines/chemistry , Glucosamine/pharmacology , RNA, Catalytic/metabolism , Anti-Bacterial Agents/chemistry , Bacillus subtilis/genetics , Bacillus subtilis/growth & development , Enzyme Activation/drug effects , Microbial Sensitivity Tests , Models, Biological , Mutation , RNA, Catalytic/geneticsABSTRACT
A novel and versatile method has been developed for modular expansion of the chemical space of nucleic acid libraries, thus enabling the generation of nucleobase-modified aptamers with unprecedented recognition properties. Reintroduction of the modification after enzymatic replication gives broad access to many chemical modifications. This wide applicability, which is not limited to a single modification, will rapidly advance the application of inâ vitro selection approaches beyond what is currently feasible and enable the generation of aptamers to many targets that have so far not been addressable.
Subject(s)
Aptamers, Nucleotide , Nucleic AcidsABSTRACT
The rise of antimicrobial resistance in human pathogenic bacteria has increased the necessity for the discovery of novel, yet unexplored antibacterial drug targets. Riboswitches, which are embedded in untranslated regions of bacterial messenger RNA (mRNA), represent such an interesting target structure. These RNA elements regulate gene expression upon binding to natural metabolites, second messengers, and inorganic ions, such as fluoride with high affinity and in a highly discriminative manner. Recently, efforts have been directed toward the identification of artificial riboswitch activators by establishing high-throughput screening assays, fragment-based screening, and structure-guided ligand design approaches. Emphasis in this review is placed on the special requirements and synthesis of new potential antibiotic drugs that target riboswitches in which dissimilarity is an important aspect in the design of potential lead compounds.
